Spectrophotometric determination of blood oxygen

Hickam, John B.; Frayser, K.R.

doi:10.1016/0002-9343(49)90598-7

Cited by 27 publications

(30 citation statements)

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“…It does not appear likely that these concepts can be carried over intact to persons with acute or chronic disease. SUM MARY 1. Observations have been made on ventilation and arterial blood changes in 11 untrained subjects during short periods of moderately severe treadmill exercise.…”

Section: Discussionmentioning

confidence: 99%

“…For determination of oxygen consumption the expired air was diverted into a Douglas bag. Gas mixtures other than air were supplied to the inspira- 1 tory side of the system from large Douglas bags. These mixtures consisted of 15% 02 in N2, tank oxygen (99.5%), and 5% CO2, 21% 0, in N2.…”

Section: Methodsmentioning

confidence: 99%

“…Arterial blood oxygen content and per cent oxygen saturation were determined by a spectrophotometric method (1). The pH of whole blood was measured with a Cambridge Model R pH meter equipped with an inclosed glass electrode.…”

Section: Methodsmentioning

confidence: 99%

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Respiratory Regulation During Exercise in Unconditioned Subjects 1

Hickam¹,

Pryor²,

Page³

et al. 1951

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Respiratory Regulation During Exercise in Unconditioned Subjects 1

Hickam¹,

Pryor²,

Page³

et al. 1951

J. Clin. Invest.

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“…9 Blood oxygen capacity was determined by a cyanmethemoglobin method. 10 In the cross-perfused hindlimb preparation, the hindlimb of a recipient dog, completely separated from its parent body except for the femoral and sciatic nerves, was perfused by the circulation of a second dog (donor) through femoralfemoral anastomoses. The femoral head was disarticulated and the free ends of excised muscle bundles were cauterized.…”

Section: Methodsmentioning

confidence: 99%

Afferent neural pathway in the regulation of cardiopulmonary responses to tissue hypermetabolism.

Liang¹,

Hood²

1976

Circulation Research

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SUMMARY We studied the role of neural transmission from hypermetabolic peripheral tissues in the regulation of cardiac output and pulmonary ventilation in chloralose-anesthetized dogs. Cross-circulation techniques with femoral-femoral or femoral-aortic anastomoses were used to produce a vascularly isolated, but normally innervated, hindlimb or lower half-body. 2,4-Dinitrophenol (DNP) was infused into the arterial side of the perfusion circuit to triple oxygen consumption and to increase lactate production by the cross-perfused area. After infusion of DNP, cardiac output and mean systemic arterial blood pressure increased, but neither heart rate nor pulmonary artery wedge pressure changed significantly. Pulmonary minute ventilation and arterial pH also increased, while arterial Pco 2 fell. These changes were abolished when the nerve connections between the perfused limb and its parent body were severed. Normal saline, when administered in a similar manner, did not increase either ventilation or cardiac output, and simple denervation without previous infusions of DNP also had no effect. These results indicate that there are receptors sensitive to metabolic changes in the tissue, and that neural transmission is an important afferent link in regulating the cardiopulmonary responses to increased tissue metabolism.BOTH CARDIAC output and pulmonary ventilation are increased during tissue hypermetabolism induced by 2,4-dinitrophenol (DNP). 1 -2 Levine and Huckabee 3 showed that the respiratory stimulation which follows DNP infusion is not a result of direct action of DNP on the brain or carotid chemoreceptors, hence the primary stimulus for these responses probably occurs in the peripheral hypermetabolic tissue from which it is transmitted to the central nervous system, heart, and lungs via neural or humoral pathways.Earlier, Ramsay 4 infused DNP into the normally innervated hindlimb of a dog (recipient dog), which was separated vascularly from its own central circulation and perfused by a second dog. He found that ventilation of the recipient dog was stimulated after DNP infusion and that this response was abolished when the nerve connections between the limb and the body were severed. These findings suggest that a neural pathway is involved in the regulation of pulmonary ventilation during tissue hypermetabolism. These findings, however, were not confirmed by Bailen and Horvath, 5 who also used a hindlimb preparation but administered a smaller dose of DNP than did Ramsay. This difference in doses might explain in part the discrepancies between their experimental results. In addition, the mass of tissue that could be rendered hypermetabolic in the hindlimb preparations is small, and the magnitude of stimulus generated in such areas by the small dose of DNP used in the experiments of Bailen and Horvath might not have been sufficient to produce a significant increase in pulmonary ventilation.In our present experiments, we attempted to study the role of neural transmission in the regulation of pulmonary ventilation and ca...

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“…Friedman venous pressure provides in general much higher gradients in the cirrhotic group than in the control subjects, the mean figure being 16 normal persons may be less than the true portal venous pressure, possibly because of the richness of the anastomotic runoff, although the observations of Paton and coworkers8 do not support such a discrepancy. Direct measurements of portal venous pressure in cirrhotic subjects have also been made2"-24 at laparotomy.…”

mentioning

confidence: 92%

Occlusive Hepatic Venous Catheterization in the Study of the Normal Liver, Cirrhosis of the Liver and Noncirrhotic Portal Hypertension

Taylor

Myers

1956

Circulation

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T HE anatomic location of the portal vein is such that measurements of portal pressure in manl have been possible only at laparotomy. The relative inaccessability of the portal vein has been a particular handicap in the study of Laennec's eirrhosis inl which the effects of deranged intrahepatic circulation with resultant portal hypertension are of great importance in the course and ultimate outcome of the disease.Various indirect methods' 2 for giving an indication of portal pressure have not proved fully satisfactory and the need for a suitable techini(qIue for estimating portal venous pressure has become more pressing with the advent of direct surgical means for attacking the problem of portal hypertension. A method for direct needling of the portal vein in unanesthetized man has recently been reported3 but its applicability would appear to be limited.

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Spectrophotometric determination of blood oxygen

Cited by 27 publications

References 0 publications

Respiratory Regulation During Exercise in Unconditioned Subjects 1

Respiratory Regulation During Exercise in Unconditioned Subjects 1

Afferent neural pathway in the regulation of cardiopulmonary responses to tissue hypermetabolism.

Occlusive Hepatic Venous Catheterization in the Study of the Normal Liver, Cirrhosis of the Liver and Noncirrhotic Portal Hypertension

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