Spectrophotometric Determination of Adefovir Dipivoxil in bulk and Pharmaceutical Formulation

Abstract

Two selective and sensitive spectrophotometric methods have been developed for the estimation of adefovir dipivoxil in bulk and pharmaceutical preparations. Adefovir dipivoxil was subjected to acid hydrolysis and the hydrolysed product used for the estimation. The methods are based on the reaction with 3-methyl-2-benzothiazolinone hydrazone in the presence of ferric chloride, to form a colored species with absorption maxima at 627 nm. The second method is based on the reaction of drug with 1,2 naphthaquinone -4-sulphonic acid sodium salt, under alkaline conditions which absorbs maximally at 454 nm. Beers law is obeyed in the concentration range of 5-25 µg/mL and 2-10 µg/mL respectively. These methods were extended to pharmaceutical formulations and there was no interference from excepients and diluents. The analytical parameters were evaluated.

Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

“…Several techniques have been explored for detection and quantification of Adefovir in pharmaceutical formulation and biological fluids such as spectrophotometry [5] , high performance liquid chromatography [6] , [7] , ion-pair liquid chromatography [8] , [9] , hydrophilic interaction liquid chromatography–tandem mass spectrometry [10] and liquid chromatography–tandem mass spectrometry [11] , [12] . Although spectrophotometry and chromatography are the most commonly employed techniques, but these involve many derivatization steps and extraction purification approaches prior to final analysis.…”