Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update

Abstract

The aim of this review is to study the main spectrophotometric methods used to evaluate total antioxidant capacity (TAC) in serum samples of dogs. Total antioxidant capacity (TAC) is an analyte frequently used to assess the antioxidant status of biological samples and can evaluate the antioxidant response against the free radicals produced in a given disease. Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC) are different assays described to determine TAC of a sample. This review explains the basis of each assay and their application in the determination of TAC in dogs, and also provides selected information about reports in humans for comparative purposes. It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.

Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

“…The methods vary widely, which can result in non-comparable results among Unauthenticated Download Date | 5/12/18 12:08 AM studies [5,18,33]. In the present study, plasma TAC values were determined by using the TEAC ABTS-based method.…”

“…The volumes of the samples needed for TAC, GSH-Px and SOD determinations on the RXDaytona biochemical analyser were 100 μL of heparin plasma, 25 μL of whole blood and 30 μL of haemolysate, respectively. The TAS kit is based on the method of Miller et al [16]), which is one of the TEAC (trolox equivalent antioxidant capacity) ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) based methods and has been previously used in dogs [18]. The results are expressed as mmol/L of Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equivalents.…”

“…Data on the reference ranges of TAC [7], GSH-Px, and SOD [8] in healthy dogs are lacking, and there are no published accepted standard reference methods for their determination. Various measurement methods have been developed for the determination of TAC, GSH-Px and SOD; however, these methods differ greatly among studies, which led to discrepancies in the results [5,[9][10][11][12][13][14][15][16][17][18].…”

Antioxidative and Antiradical Seasonal Distinctives of Sea Buckthorn Sprouts

“…The given method shows the amount of low molecular weight antioxidants but does not include any compounds with thiol groups. 15 Based on Figure 3, ethanolic extracts had the highest reducing power followed by water. Autumn sample had the significantly highest reducing power in the range of 165.…”

Oxidative status and lipid profile in mono- and co-infection with canine monocytic ehrlichiosis

“…Methods for determining the oxidative status include direct and indirect measurements of oxidants and antioxidants. Despite some disadvantages, measurements of TAC and TOS concentrations are a practical way to identify the oxidative status of an organism (13,32). One of the products formed during the oxidative damage-induced decomposition of polyunsaturated fatty acid is MDA (8).…”