Spectrophotometric Assay Methods for Determination of Amiloride: A Charge-Transfer Reaction Approach with 2,3-Dichloro-5,6-Dicyano-1,4-Benzoquinone and p-Chloranilic Acid Reagents

Abstract

The present study proposes two straightforward, responsive and inveterate spectrophotometric techniques for the detection of amiloride hydrochloride. The methodologies are mainly relay on the generation of charge transfer complex, with the reagents 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (Method A) and p-chloranilic acid (Method B), hence an enduring 1:1 stoichiometric complex were accomplished, which are yellow and pink colour correspondingly. The generated chromogens optical densities were measured at 476 and 508 nm respectively. Beer's law been tested in the range of 5-30 µg.ml -1 and 1-6 µg.ml -1 . The other analytical parameters like, Limit of Detection (LOD), Limit of Quantification (LOQ), accuracy and precisions were examined. The mentioned approaches were successfully adopted for the determination of the amiloride hydrochloride in tablet formulation with high accuracy, better recoveries and acceptable relative standard deviation.