Spectrophotometric and spectrofluorimetric methods for the determination of pregabalin in bulk and pharmaceutical preparation

Monitoring of Pregabalin in Pharmaceutical Formulations and Human Serum Using UV and RP-HPLC Techniques: Application to Dissolution Test Method

“…Literature survey has revealed few analytical methods of its determination in bulk drugs and pharmaceutical formulations [5,[8][9][10][11][12] but all of these methods are very expensive as they require long and tedious pretreatment of the samples, including extraction with solvent and derivatization for its analysis, so there was a need for the development of a simple, economical and least time consuming HPLC/ UV method for the quantification of PGB in bulk, pharmaceutical formulations and human plasma. Hence, an attempt has been made to develop a novel, sensitive, simple, efficient, economic, selective having a short time and lower limits of quantification and detection.…”

“…Its lowest effective dose is 150 mg/day. Onal and Saqirli [5] determined pregabalin in bulk and pharmaceutical preparations by spectrophotometric and spectro florometric techniques. Other methods for determination of pregambalin include ultra-high pressure liquid chromatography (UPLC) and tandem mass spectrometric (MS/MS) in whole blood and urine [6] NMR technique and capillary electrophoresis [7].…”

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

“…1) The analytical method development for PGB, GBP and VGB determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been proposed [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and used for their determination in bulk or pharmaceutical dosage forms, plasma, serum, and urine. These methods employed derivatization reagents such as fluorescamine, [2][3][4][17][18][19] 7-chloro-4-nitrobenzofurazan, [5][6][7] 1-fluoro-2,4-dinitrobenzene, 8) o-phtaldialdehyde, 9,10) 2,4,6-trinitrobenzene sulfonic acid, 11,12) and 9-fluorenylmethylchloroformate.…”

“…13) The resulted derivatives were measured using non-separation spectrophotometric 5,6,[15][16][17] and spectrofluorimetric methods. 2,[5][6][7]17,19) Moreover, HPLC 3,[8][9][10][11][12][13][14]18) and capillary electrophoresis (CE) methods 4) using precolumn derivatization with either UV or fluorescent detector were also used.…”

“…In addition method should meet validation criteria in terms of precision, accuracy, robustness etc., and most of all it has to offer highthroughput. So far developed methods for determination of PGB, 3,5,17,19) GBP, 2,7,8,15,16,18,[20][21][22][23][24] and VGB 2,6,7,18,25) use from a few µL to approximately 1 mL of sample. The sample preparation is the time-consuming step in derivatization methods and in case of PGB, GBP, and VGB derivatization can take from few seconds to 50 min.…”