Spectrophotometric and spectrofluorimetric methods for analysis of acyclovir and acebutolol hydrochloride

Abstract

Simple and sensitive spectrophotometric and spectrofluorimetric methods are described for analysis of acyclovir and acebutolol hydrochloride. The proposed methods are based on oxidation of the selected drugs with cerium(IV) ion in acidic medium with subsequent measurement of either the decrease in absorbance at 320nm or the fluorescence intensity of the produced cerous(III) ion at 361-363nm (excitation at 250nm). Beer's law obeyed from 2 to 8, 0.25 to 2.5microgcm-1 acyclovir, 1 to 7 and 0.25 to 2.5microgml-1 acebutolol hydrochloride, using the spectrophotometric and spectrofluorimetric method, respectively. The proposed method were successfully applied for determination of the selected drugs in their pharmaceutical preparations with good recoveries.

Stability evaluation and sensitive determination of antiviral drug, valacyclovir and its metabolite acyclovir in human plasma by a rapid liquid chromatography–tandem mass spectrometry method

“…pharmaceuticals [7][8][9][10][11], human plasma [12][13][14][15][16][17], serum [18] and maternal plasma, amniotic fluid, fetal and placental tissues [19]. High-performance capillary electrophoresis has also been used for the determination of ACV in urine [20] and plasma [21].…”

Simultaneous Kinetic-Spectrofluorometric Determination of Levodopa and Carbidopa Using Partial Least-Squares Regression

“…The influence of the Ce(IV) concentration was studied in the range between 1 ¥ 10 -5 and 1 ¥ 10 -4 mol L -1 . Higher concentrations were not evaluated because of the possibility of a fluorescence inner filter, since Ce(IV) absorbs at 350 nm (lmax about 320 nm), 28 the same l at which Ce(III) emits. The rate of the reaction increases with the concentration of Ce(IV) up to 2 ¥ 10 -5 and 3 ¥ 10 -5 mol L -1 for carbidopa and levodopa, respectively.…”

“…6 Ce(IV) has been used as an oxidizing agent for the determination of several pharmaceuticals. [22][23][24][25][26][27][28][29] It can be easily reduced to Ce(III), which shows a characteristic fluorescence in dilute sulfuric acid. This reaction could be implemented as an expeditious method for the indirect fluorometric determination of several pharmaceutical species that cannot be directly determined by fluorescence.…”

Simultaneous determination of aciclovir, ganciclovir, and penciclovir in human plasma by high-performance liquid chromatography with fluorescence detection

“…rescence detection [23,24,29,33,36,37,39,40,45,46,49], and tandem mass spectrometry [47,53]. However, the LLOQs for UV detection were above 50 and 200 ng/ml for GCV, and PCV, respectively, which may not be sensitive enough for pharmacokinetic studies.…”

“…Hence, this complicates analysis and requires using a highly selective analytical method. Some analytical methods have been proposed for individual measurement of ACV [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37], GCV [38][39][40][41][42][43][44][45], and PCV [46][47][48]. Other techniques have been developed for simultaneous determination of ACV and GCV [49][50][51], valacyclovir and ACV [52], or valganciclovir, and GCV [53], through high-performance liquid chromatography (HPLC) in biological fluids.…”