“…These include ultraviolet (UV) spectrophotometry [4,10,17], fluorimetry [13], polarography [1], voltammetry [35], colorimetry [39], capillary zone electrophoretic method [24], thin layer chromatography with densitometry [5], and high performance liquid chromatography (HPLC)-UV detection [34]. However, many of these techniques are not suitable for measuring MEL concentrations in biological samples for PK applications due to both poor sensitivity and selectivity.…”
An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic-lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45:55 (v:v)] on a Nova-Pak C18 4-µm (300 × 3.9 mm) column. Retention times for MEL and piroxicam were 8.03 and 5.56 min, respectively. Peak area ratios of MEL to the internal standard (IS) were used for regression analysis of the calibration curve, which was linear from 10 to 1,000 ng/mL (r2 > 0.9998). Average absolute recovery rates were 91% and 96% for MEL and the IS, respectively. This method had sufficient sensitivity (lower quantitation limit of 10 ng/mL), precision, accuracy, and selectivity for routine analysis of MEL in koala plasma using 250-µL sample volumes. Our technique clearly resolved the MEL peak from the complex koala plasma matrix and accurately measured MEL concentrations in small plasma volumes.
“…These include ultraviolet (UV) spectrophotometry [4,10,17], fluorimetry [13], polarography [1], voltammetry [35], colorimetry [39], capillary zone electrophoretic method [24], thin layer chromatography with densitometry [5], and high performance liquid chromatography (HPLC)-UV detection [34]. However, many of these techniques are not suitable for measuring MEL concentrations in biological samples for PK applications due to both poor sensitivity and selectivity.…”
An improved method to determine meloxicam (MEL) concentrations in koala plasma using reversed phase high performance liquid chromatography equipped with a photo diode array detector was developed and validated. A plasma sample clean-up step was carried out with hydrophilic-lipophilic copolymer solid phase extraction cartridges. MEL was separated from an endogenous interference using an isocratic mobile phase [acetonitrile and 50 mM potassium phosphate buffer (pH 2.15), 45:55 (v:v)] on a Nova-Pak C18 4-µm (300 × 3.9 mm) column. Retention times for MEL and piroxicam were 8.03 and 5.56 min, respectively. Peak area ratios of MEL to the internal standard (IS) were used for regression analysis of the calibration curve, which was linear from 10 to 1,000 ng/mL (r2 > 0.9998). Average absolute recovery rates were 91% and 96% for MEL and the IS, respectively. This method had sufficient sensitivity (lower quantitation limit of 10 ng/mL), precision, accuracy, and selectivity for routine analysis of MEL in koala plasma using 250-µL sample volumes. Our technique clearly resolved the MEL peak from the complex koala plasma matrix and accurately measured MEL concentrations in small plasma volumes.
“…Because of solubility of meloxicam in acidic medium, it may cause local gastrointestinal adverse events [5]. Up to now, there are a limited number of techniques described for the determination of meloxicam in pharmaceuticals formulations only including ultraviolet spectrophotometry [6], flow injection [7] and high-performance liquid chromatography (HPLC) with UV detector [8,9]. The Chinese pharmacopoeia method for conventional assaying meloxicam is based on neutralization titration in the solvent of ethanol [10].…”
Abstract:The improvement of electrochemical detection of meloxicam is presented by modification of a glassy carbon electrode with anionic layer of cysteic acid providing electrostatic accumulation of the analyte onto the electrode surface. The modification formed by electrochemical oxidation of L-cysteine was performed by cycling potential in cysteine solution. The anodic peak current obtained at + 1.088 V (vs. Ag/AgCl) by voltammetry was linearly dependent on the meloxicam concentration in the range of 4.3 × 10 -8 ~ 8.5 × 10 -6 M in the B-R buffer solution (0.04 M, pH 1.86) with a correlation coefficient of 0.999. The detection limit (S/N = 3) is 1.5 × 10 -9 M. The low-cost modified electrode shows good sensitivity, selectivity and stability and has been applied to the determination of meloxicam in pharmaceutical formulation and spiked serum with satisfactory results. The electrochemical reaction mechanism of meloxicam was discussed.
“…change in methyl to ethyl at 2 positions causes complete suppression of activity (Lemke & Williams 2008). Several analytical methods for the determination of meloxicam by flourimetry (Hassan 2002), capillary electrophoresis (Nemutlu & Kir 2003), spectrophotometry (Hassan 2002), HPLC (Arayne et al 2005; Farzana & Pradeep 2009; Damle et al 2009), LC/MS (Wiesner et al 2003) and polarographic (Altıokka et al 2000; Radi et al 2001a; Altınöz et al 2002; Radi et al 2001b; Rao et al 2005) have been reported. The aim of the present work was to develop and validate a sensitive RP-HPLC method that can be implemented for the quantification of meloxicam in bulk as well as in its tablet dosage forms.Figure 1
Chemical structure of Meloxicam.…”
A novel, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantification of Meloxicam in bulk and tablet dosage form with greater precision and accuracy. Separation was achieved on Develosil ODS HG-5 RP C18, (15 cm × 4.6 mm i.d. 5 μm) column in isocratic mode with mobile phase consisting of acetonitrile: phosphate buffer(pH 3.4) (60:40) with a flow rate of 1 mL/min. The detection was carried out at 268 nm. The retention time of Meloxicam was found to be 2.09 min. The method was validated as per ICH guidelines. Linearity was established for Meloxicam in the range 20 – 120 μg/ml with R2 value 0.996. The percentage recovery of Meloxicam was found to be in the range 99.99-100.46%. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the estimation of the drug in bulk and tablet dosage forms. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible for the determination of Meloxicam for Quality Control level.
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