Spectrofluorometric Method for the Determination of Sulfhydryl and Disulfide Groups in Milk Proteins

Vakaleris, D.G.; Pofahl, T.R.

doi:10.3168/jds.s0022-0302(68)87237-6

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Al-pan[l-(2-pvridylazo)-1

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2001

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Cited by 6 publications

(1 citation statement)

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“…Glutathione in biological materials has been analyzed by fluorometry after condensation with o-phthaldialdehyde (421). Another fluorometric determination for glutathione or other sulfhydryl or disulfide compounds is based on the quenching of the fluorescence of mercurated fluorescein derivatives (705,706,747). Histidine was determined by a modification of the histaminephthalaldehyde method, and the errors caused by many possible interferences were evaluated (13).…”

Section: Al-pan[l-(2-pvridylazo)-mentioning

confidence: 99%

Fluorometric analysis

White¹,

Weissler²

1970

Anal. Chem.

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Section: Al-pan[l-(2-pvridylazo)-mentioning

confidence: 99%

Fluorometric analysis

White¹,

Weissler²

1970

Anal. Chem.

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Analytical applications of o-hydroxymercuribenzoic acid, dithiofluorescein and mercurated fluorescein

Wroński

1977

Talanta

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Characterization of hemolytic activity of Staphylococcus aureus strains isolated from bovine mastitic milk

Ali-Vehmas¹,

Vikerpuur²,

Pyörälä³

et al. 2001

Microbiological Research

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Beta (beta) and delta (delta)-hemolysin of Staphylococcus aureus strains were cultured in vitro in milk lactoserum (whey) prepared from both healthy and mastitis bovine milk. Production of beta- and delta-hemolysins were detected in 12 out of 50 strains studied. The association between N-acetyl-beta-D-glucosaminidase (NAGase) activity, plasmin activity (PL) and trypsin inhibitory capacity (TIC), known as inflammatory indicators for mastitis, and hemolytic activity were also studied. Mastitic milk decreased directly the lytic effect of both beta-and delta-hemolysins of S. aureus on hemolytical blood agar plates. S. aureus in healthy milk samples produced more beta-hemolysin (3 times) and delta-hemolysin (2 times) when compared to S. aureus supernatants in milk from infected quarters. Furthermore, beta- and delta-hemolysis correlated negatively with TIC and NAGase and PL activities. Addition of reduced glutathione (GSH) or beta-mercaptoethanol into the artificial medium enhanced hemolysins activity.

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Spectrofluorometric Method for the Determination of Sulfhydryl and Disulfide Groups in Milk Proteins

Cited by 6 publications

References 16 publications

Fluorometric analysis

Fluorometric analysis

Analytical applications of o-hydroxymercuribenzoic acid, dithiofluorescein and mercurated fluorescein

Characterization of hemolytic activity of Staphylococcus aureus strains isolated from bovine mastitic milk

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