Spectrofluorometric Determination of Protoporphyrin IX in Cells Using Acridine as Internal Standard

Lee, Ho-Suk; Lee, Jee-Bum; Yun, Sook Jung; Choi, Jee Young; Oh, Jeong Seok; Park, Hyoung-Ryun

doi:10.5012/bkcs.2006.27.7.1067

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…This method was modified from the method of Lee et al [73]. Parental HSC-1 and resistant cells were seeded in 12-well plates (2 × 10 5 cells/well) and cultured for 24 h. Next, cells were incubated with a 2 mM MAL solution for 4 h as described above.…”

Section: Intracellular Content Of Ppixmentioning

confidence: 99%

Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

León

Buchegger

Silva

et al. 2020

IJMS

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Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some skin lesions have not fully responded to this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant skin cancer. In this study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, cell death, reactive oxygen species (ROS) detection and relative gene expression in PDT-resistant HSC-1 cells. PDT-resistant HSC-1 cells show a low quantity of protoporphyrin IX and low levels of ROS, and thus a low rate of death cell. Furthermore, the resistant phenotype showed a downregulation of HSPB1, SLC15A2, FECH, SOD2 and an upregulation of HMBS and BIRC5 genes. On the other hand, epigallocatechin gallate catechin enhanced the MAL-PDT effect, increasing levels of protoporphyrin IX and ROS, and killing 100% of resistant cells. The resistant MAL-PDT model of skin cancer squamous cells (HSC-1) is a reliable and useful tool to understand PDT cytotoxicity and cellular response. These resistant cells were successfully sensitized with epigallocatechin gallate catechin. The in vitro epigallocatechin gallate catechin effect as an enhancer of MAL-PDT in resistant cells is promising in the treatment of difficult skin cancer lesions.

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Section: Intracellular Content Of Ppixmentioning

confidence: 99%

Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

León

Buchegger

Silva

et al. 2020

IJMS

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Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

Kars

Kara

Gündoğdu

et al. 2014

Biomedicine & Pharmacotherapy

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Susceptibility to 5‐Aminolevulinic Acid Based Photodynamic Therapy in WHO I Meningioma Cells Corresponds to Ferrochelatase Activity

Hefti

Holenstein

Albert

et al. 2010

Photochem & Photobiology

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5-Aminolevulinic acid (5-ALA) is a natural precursor of protoporphyrin IX (PpIX), which can be used as a photosensitizer in photodynamic therapy (PDT). Accumulation of PpIX in benign meningioma cells has been observed previously, its exploitation for PDT, however, was discouraged by inconsistent results. To evaluate PDT for benign meningiomas, we investigated PpIX synthesis in two human meningioma cell lines (HBL-52 and BEN-MEN-1), their respective extracellular loss of PpIX and corresponding ferrochelatase (FECH) activity as well as their susceptibility to PDT. We demonstrated PpIX production after 5-ALA administration and minor loss to the extracellular space in both cell lines. However, significantly more (up to five times) PpIX was accumulated in BEN-MEN-1 as compared with HBL-52 cells. FECH activity was 2.7-fold higher in HBL-52 compared with BEN-MEN-1 cells and accordingly higher FECH levels could be shown in HBL-52 cells by Western blot analysis. BEN-MEN-1 cells were much more sensitive to PDT and cells could be almost completely killed by irradiation doses of 2 J cm(-2) , whereas HBL-52 showed only an insufficient response at this irradiation dose. We conclude that differences in intracellular PpIX concentrations between HBL-52 and BEN-MEN-1 benign meningioma cells were mainly due to differences in FECH activity and that these differences correspond to their susceptibility to 5-ALA-induced PDT.

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Spectrofluorometric Determination of Protoporphyrin IX in Cells Using Acridine as Internal Standard

Cited by 4 publications

References 8 publications

Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

Femtosecond laser induced photodynamic therapy on 5-ALA treated SKMEL-30 cells: An efficient theranostic strategy to combat melanoma

Susceptibility to 5‐Aminolevulinic Acid Based Photodynamic Therapy in WHO I Meningioma Cells Corresponds to Ferrochelatase Activity

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