Spectrofluorimetric determination of fluoroquinolones in pharmaceutical preparations

Ulu, Sevgi Tatar

doi:10.1016/j.saa.2008.08.017

Cited by 46 publications

(23 citation statements)

References 45 publications

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“…Various methods have been reported for the analysis of moxifloxacin in pharmaceutical dosage forms by flow injection with chemiluminescence detection [3], atomic absorption spectrometry [4], atomic absorption spectroscopy, conductometry and colorimetry [5], spectrophotometry [4,[6][7][8][9][10], kinetics spectrophotometry [4,[11][12][13], voltammetry [14,15], differential pulse polarography [16], spectrofluorimetry [17][18][19] and capillary electrophoresis [20,21]. Chromatographic methods such as reversed-phase HPLC/fluorescence [22][23][24], HPLC with ultra violet detection [25][26][27] and high-performance thin-layer chromatography [28] have been reported for the estimation of moxifloxacin hydrochloride in pharmaceutical products.…”

Section: Introductionmentioning

confidence: 99%

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

Ashour¹,

Kattan²

2016

SOJPPS

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Research articleperformance liquid chromatography with fluorescence [29,30] or UV detection [31][32][33][34][35], liquid chromatography tandem mass spectrometry (LC/MS/MS) [36,37] and capillary electrophoresis [38,39] are also reported for determination of moxifloxacin hydrochloride from human body fluids. British Pharmacopoeia (BP) [40] employs HPLC method for the assay of moxifloxacin hydrochloride in pharmaceutical preparations. Further, the BP describes no environmentally friendly chromatographic procedure since acetonitrile is one of the components of mobile phase.This work describes a rapid and sensitive RP-HPLC method with UV detection for determination of moxifloxacin hydrochloride in bulk drug and tablet formulation with low cost of mobile phase, which was used for the first time in this work. This method serves as an alternative to the methods described in pharmacopoeias and can be conveniently used for routine quality control analysis of moxifloxacin. Experimental MaterialsHPLC grade methanol (Merck, Germany), formic acid (AR grade, Surechem Products LTD, England) and water (HPLC grade, Merck). Moxifloxacin hydrochloride (MOXF) pure drug substance, its purity was found to be 99.7%, and rosuvastatin calcium (ROSV) was obtained from Matrix Laboratories Limited and MSN Laboratories Limited (India), respectively. Tablets were purchased from Syrian market, containing moxifloxacin hydrochloride 400 mg per tablet. AbstractSimple, rapid, sensitive and accurate method for determination of moxifloxacin HCl (MOXF) in bulk drug and tablet formulation was developed and validated by using HPLC. Rosuvastatin calcium (ROSV) was used as internal standard. Chromatography was performed with mobile phase consisted of 0.1 M formic acid (pH 2.80) and methanol (30:70, v/v), with a flow rate of 1.0 mL/min, Nucleodur C18 column (250 mm×4.6 mm i.d., 5 m particle size) and photodiode array (PDA) detection at 280 nm. The method was validated for linearity, specificity, accuracy, precision, stability and robustness. Linearity range was 1.12-590.0 µg/mL with limit of detection value of 0.23 µg/mL. The precision of the method was demonstrated using intraand inter-day assay RSD% values which were less than 2%, while the recovery was 99.11-103.85%. The developed method was very rapid with a run time of 3 min and found to be successively applied for the quality control of MOXF in bulk drug and tablets.

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Section: Introductionmentioning

confidence: 99%

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

Ashour¹,

Kattan²

2016

SOJPPS

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“…These procedures include spectrophotometry and/or fluorimetry [3][4][5][6], high performance thin layer chromatography [7], voltammetry [8], HPLC [9][10][11], and LC/MS Abstract .HCl [12,13]. Forced degradation study was not reported in these articles [4][5][6][7][8][9][10][11][12][13]; in addition, a paper on the stability indicating HPLC assay of ATM in presence of unknown degradation products was developed [14]. However, the paper did not deals neither with the determination of ATM in spiked plasma nor with analysis of the drug in presence of known degradates using (DSF) derivative synchronous fluorometry.…”

Section: Introductionmentioning

confidence: 99%

Validated Stability-Indicating UPLC and Derivative Synchronous Fluorescence Spectroscopy Methods for the Determination of Atomoxetine Hydrochloride in Pharmaceutical Preparation

Soliman¹,

El-Agizy²

2014

Pharm Anal Acta

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“…They are particularly used in bacterial urinary infections and also for infections whose antimicrobes possess great resistance. The broad spectrum antimicrobial activity, excellent bioavailability, good tissue penetration and long plasmatic stocking life, all have made fluoroquinolones, a special class of drugs [8].…”

Section: Introductionmentioning

confidence: 99%

“…Thorough study of the literature reveals that several methods have been developed for the determination of fluoroquinolones in bulk, pharmaceutical dosage form and biological samples based on wide array of instru-ments like spectrophotometric [9][10][11], HPLC [12,13], capillary electrophoresis [14], electrochemical [15] and spectrofluorimetric [8,[16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…The charge-transfer methods for fluoroquinolones reported are based on the use of 2,3,5,6-tetrachloro-p-benzoquinone with enrofloxacin, levofloxacin and ofloxacin [8], 7,7,8,8-tetracyanoquinodimethane with ciprofloxacin, norfloxacin, pefloxacin and fleroxacin [16] as well as with ofloxacin, levofloxacin and lemofloxacin [17]. Chloranilic acid is used with lemofloxacin, fleroxacin, ciprofloxacin and norfloxacin as electron donor [18].…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Spectrofluorimetric Method for Determination of Fluoroquinolones through Charge-Transfer Complex Formation

Shah¹,

Jan²,

Ullah³

et al. 2013

AJAC

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A sensitive spectrofluorimetric method was developed for determination of ciprofloxacin (CPFX), levofloxacin (LEV), gatifloxacin (GAT) and moxifloxacin (MOX) in pure, commercial formulations, human urine and plasma. The method is based on charge-transfer (CT) complex with chloranilic acid. Fluorescence intensity of the complexes was measured at emission wavelength ranging from 445 -492 nm with excitation wavelengths from 285 -330 nm. At optimum experimental conditions, a linear calibration plot was obtained in the concentration range of 20 -1000 ng·mL , 20 -800 ng·mL −1 and 20 -100 ng·mL −1 for CPFX, LEV, MOX and GAT, respectively with good correlation coefficient in the range of 0.9929 -1.0 in methanolic medium. The limit of detection and limit of quantification were found to be 5 ng·mL −1 and 18 ng·mL −1 for CPFX, 12 ng·mL −1 and 40 ng·mL −1 for LEV, 8 ng·mL −1 and 19 ng·mL −1 for MOX, 6 ng·mL −1 and 19 ng·mL −1 for GAT, respectively. The method was found free of interferences from excipients used as additive in pharmaceutical preparations, some common cations and compounds present in urine and plasma as well as co-administered analgesic, vitamins and other drugs. The method was successfully applied for quantification of selected fluoroquinolones in commercial formulations and also in spiked human urine and plasma samples with percent recoveries of 100.0 ± 1.56 and 100.2 ± 1.29 respectively.

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Spectrofluorimetric determination of fluoroquinolones in pharmaceutical preparations

Cited by 46 publications

References 45 publications

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

New, Simple and Validated RP-HPLC Method for Quality Control of Moxifloxacin

Validated Stability-Indicating UPLC and Derivative Synchronous Fluorescence Spectroscopy Methods for the Determination of Atomoxetine Hydrochloride in Pharmaceutical Preparation

Sensitive Spectrofluorimetric Method for Determination of Fluoroquinolones through Charge-Transfer Complex Formation

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