Spectrofluorimetric assessment of plasma dipyridamole stability: Sample storage for multicenter clinical trials?

Serebruany, Victor L.; Pokov, Ilya; Hanley, Daniel F.

doi:10.1016/j.thromres.2008.07.002

Cited by 5 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, assessing drug concentrations serially, at different time points in just one patient undergoing treatment with ERD takes about 4 hours (h) of preparation and fluorometry time representing the major challenge preventing this informative technique to spread beyond high-profile academic facilities. However, maintaining durability of plasma dipyridamole concentration over time, and potential storage of the drug within lipophilic cell membranes may represent an attractive alternative to the pharmacokinetics of currently available antithrombotics (14). Considering alarmingly high non-compliance rates for antithrombotics in general (15), and ERD in particular (16) missing even one daily dose may represent a serious challenge for maintaining efficacy.…”

Section: Introductionmentioning

confidence: 99%

Distribution of dipyridamole in blood components among post-stroke patients treated with extended release formulation

et al. 2009

Self Cite

View full text Add to dashboard Cite

Extended release dipyridamole (ERD) is widely used in patients after ischaemic stroke; however, the ability of this antithrombotic agent to be stored in different blood cells has never been explored in post-stroke patients. We hypothesised that since ERD is known to be highly lipophilic, the drug may be present not only in plasma, but also accumulated in platelets, leukocytes, and erythrocytes. Fifteen patients after documented ischaemic stroke were treated with Aggrenox (ERD and low-dose aspirin combination) BID for 30 days, and 12 of them completed the study. ERD concentrations in blood cells and platelet-poor plasma were measured by spectrofluorimetry at Baseline, Day 14, and Day 30 after the initiation of therapy. The background level of spectrofluorometry readings differs slightly among the blood components (132-211 ng/ml) due to the differences in the preparation of samples and cell isolation techniques. As expected, two weeks of ERD therapy produced steady-state plasma concentration of dipyridamole already at Day 14 (1,680 +/- 542 ng/ ml), followed by a slight not significant decrease at one month (1,619 +/- 408 ng/ml). Two weeks of therapy was sufficient to achieve a consistent dipyridamole accumulation in erythrocytes (361 +/- 43 ng/ml), but not in platelets (244 +/- 78 ng/ml), or leukocytes (275 +/- 49 ng/ml). In fact, white blood cells continued dipyridamole intake beyond 14 days period, and this increase (398 +/- 66 ng/ml) was significant (p = 0.02) at 30 days. Treatment with ERD in post-stroke patients resulted not only in achievement of therapeutic plasma dipyridamole concentrations, but also deposition of the drug in erythrocytes and leukocytes, but not in platelets. If confirmed, these data will affect our better understanding of dipyridamole pleiotropy, and may explain long-term benefit of ERD formulation.

show abstract

Section: Introductionmentioning

confidence: 99%

Distribution of dipyridamole in blood components among post-stroke patients treated with extended release formulation

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…Spectrophotometry and conductometry have low sensitivity and selectivity. On the other hand, methods, such as phosphorimetry [13], chemiluminescence [14], and fluorimetry [15], have shown the achievement of lower limits of detection and higher selectivity and sensitivity compared to other traditional detection methods of DIP.…”

Section: Introductionmentioning

confidence: 99%

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

et al. 2023

View full text Add to dashboard Cite

A new method for the determination of the antiplatelet drug dipyridamole (DIP) in pharmaceuticals using a molecularly imprinted polymer (MIP)-modified pencil graphite electrode (PGE) is proposed. The modified electrode was prepared simply and rapidly by electropolymerization of caffeic acid (CA) in the presence of DIP and subsequent DIP extraction with ethanol, resulting in a cost-effective, eco-friendly disposable modified electrode (MIP_PGE). Several working conditions (monomer and template concentration, number of voltametric cycles, scan rate extraction time, and solvent) for the MIP_PGE preparation were optimized. The differential pulse voltammetric (DPV) oxidation signal of DIP obtained at MIP_PGE was 28% higher than that recorded at bare PGE. Cyclic voltammetry emphasized DIP irreversible, pH-dependent, diffusion-controlled oxidation at MIP_PGE. Differential pulse and adsorptive stripping voltammetry at MIP_PGE in phosphate buffer solution pH = 7.00 were applied for the drug quantitative determination in the range of 1.00 × 10−7–1.00 × 10−5 and 1.00 × 10−8–5.00 × 10−7 mol/L DIP, respectively. The obtained limits of detection were at the tens nanomolar level.

show abstract

“…In order to improve their performance, athletes have sometimes fraudulently consumed DIP, but the uncontrolled administration of this drug can have serious side effects [13]. DIP's effects in the body last a long time and its concentration in blood and urine is usually very low [14,15], but DIP plasma content remains unchanged over 2 weeks of storage [16]. Thus, the development of sensitive and selective methods for DIP assay in pharmaceutical and biological samples is required.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the development of sensitive and selective methods for DIP assay in pharmaceutical and biological samples is required. As DIP ( Figure 1) contains a stable heteroaromatic double * Correspondence: i _ g _ david@yahoo.com central system responsible for its absorption and fluorescence characteristics [17], several analytical methods exploiting these properties [18][19][20][21], as well as other luminescence-based methods [13][14][15][16][17][18][19][20][21][22], have been developed for its quantification. On the other hand, several chromatographic methods with either spectrometric [23][24][25] or electrochemical [26] detection were reported for DIP analysis.…”

Section: Introductionmentioning

confidence: 99%

Novel voltammetric investigation of dipyridamole at a disposable pencil graphiteelectrode

David¹,

Iordache²,

Popa³

et al. 2019

Turk J Chem

View full text Add to dashboard Cite

The present paper describes the voltammetric analysis of dipyridamole (DIP) at a cheap, disposable pencil graphite electrode (PGE). The working conditions were optimized with regard to the electrode material and the supporting electrolyte. Cyclic voltammetric investigations emphasized that DIP is irreversibly oxidized at the PGE. The electrode process is pH-dependent and controlled by both diffusion and adsorption. For DIP quantitative determination a differential pulse voltammetric (DPV) method in phosphate buffer solution pH 7.00 was developed. DIP's oxidation peak current varied linearly with the analyte concentration, presenting two linear ranges, namely 5.00 × 10 −7-2.50 × 10 −5 M and 2.50 × 10 −5-2.50 × 10 −4 M, with detection and quantification limits of 1.21 × 10 −7 M and 4.03 × 10 −7 M DIP, respectively. The newly developed DPV method using the inexpensive, disposable PGE was successfully applied for the simple and rapid determination of DIP from pharmaceutical formulations.

show abstract

Spectrofluorimetric assessment of plasma dipyridamole stability: Sample storage for multicenter clinical trials?

Cited by 5 publications

References 8 publications

Distribution of dipyridamole in blood components among post-stroke patients treated with extended release formulation

Distribution of dipyridamole in blood components among post-stroke patients treated with extended release formulation

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

Novel voltammetric investigation of dipyridamole at a disposable pencil graphiteelectrode

Contact Info

Product

Resources

About