Spectroelectrochemical Determination of Isoprenaline in a Pharmaceutical Sample

Abstract

UV/Vis absorption spectroelectrochemistry (SEC) is a multi-response technique that has been commonly used for the characterization of materials and the study of reaction mechanisms. However, it has been scarcely used for quantitative purposes. SEC allows us to obtain two analytical signals simultaneously, yielding a dual sensor in just one experiment. In the last years, our group has developed new devices useful for analysis. In this work, a SEC device in parallel configuration, based on optical fibers fixed on screen-printed electrodes, was used to determine isoprenaline in a commercial drug, using both, the electrochemical and the spectroscopic signals. In this commercial drug, isoprenaline is accompanied in solution by other compounds. Among them is sodium metabisulfite, an antioxidant that strongly interferes in the isoprenaline determination. A simple pretreatment of the drug sample by bubbling wet-air allows us to avoid the interference of metabisulfite. Here, we demonstrate again the capabilities of UV/Vis absorption SEC as double sensor for analysis and we propose a simple pretreatment to remove interfering compounds.

Therapeutic Effect of Ultrasound Combined With Porous Lipid Clioquinol/PLGA Microbubbles on Ferroptosis in HL-1 Cardiac Cell Induced by Isoproterenol Attack

“…Erastin could mediate ferroptosis through a variety of molecules including the cystine-glutamate transport receptor (system XC−), the voltage-dependent anion channel, and p53 (Yang et al, 2020;Liu et al, 2021). ISO, a synthetic catecholamine and nonselective β-adrenergic receptor agonist, was widely used in a reproducible and well-standardized preclinical model of cardiac remodeling and dysfunction mainly due to excess production of reactive oxygen species by persistent β-adrenergic stimulation (Olmo et al, 2020;Zeng et al, 2020). Previously, Liu et al demonstrated that ferroptosis-like cell death was observed in erastin-or ISO-treated H9c2 myocytes in vitro and in rats with aortic banding inducing HF, characterized by reduced cell viability with increased lipid peroxidation and labile iron pool (Liu et al, 2018).…”

UV/Vis absorption spectroelectrochemistry of folic acid

“…UV/Vis-SEC measurements were performed using a customized UV-VIS SPELEC instrument (Metrohm-DropSens), employing the experimental setup described previously [1,5]. A deuterium lamp was used because the reagents and products of this electrochemical process absorb electromagnetic radiation in the UV region.…”

“…However, it is well-known that the use of two or more techniques can be much more helpful in the determination of a molecule. Techniques such as spectroelectrochemistry (SEC) are extremely useful analytical tools in the quantification of molecules and have proven to be very useful when dealing with complex samples or when there is a large matrix effect [1][2][3][4][5]. SEC encompasses a group of analytical techniques that are characterized by the simultaneous recording of electrochemical and spectroscopic signals [6][7][8][9][10][11].…”

“…SEC in general, and UV/Vis-SEC in particular, is a group of instrumental techniques commonly used in the characterization of new materials and/or molecules, in the study of electron transfer processes, or in the study of reaction mechanisms [6,[14][15][16][17][18][19][20][21][22]. However, the great potential of these multi-response techniques for quantitative analysis has not been enough explored [1,2,4,5,12,[23][24][25][26]. Among all SEC techniques, the coupling of electrochemistry and UV/Vis absorption spectroscopy is the most successful one in the field of electroanalysis.…”

Bowl‐shaped hollow N‐doped carbon as an electrochemical platform for the highly selective and sensitive electrochemical detection of isoprenaline in pharmaceutical injection

“…Isoprenaline (4-[1-hydroxy-2-[(1-methylethyl)amino]ethyl]-1,2-benzenediol), a β agonist, belongs to the catechol class of drugs and has little effect on alpha receptors6 [1]. As a potent two-point neurotransmitter, it has been used in the treatment of the neurological disorders [2].…”