Spectrally resolved confocal microscopy using lanthanide centred near-IR emission

Liao, Zhiyu; Tropiano, Manuel; Mantulnikovs, Konstantins; Faulkner, Stephen; Vosch, Tom; Sørensen, Thomas Just

doi:10.1039/c4cc09618e

Cited by 37 publications

(44 citation statements)

References 26 publications

(24 reference statements)

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“…[7][8][9] Various types of luminescent lanthanide complexes have been reported to date. [10][11][12][13][14][15][16][17][18][19][20][21] Lanthanide complexes with asymmetric coordination structures exhibit high emission quantum yields (Φff) and large radiative rate constants (kr). [22,23] Lanthanide complexes generally provide eightcoordinate square anti-prism structures that are categorized as D4d in point group theory (8-SAP, Figure 1a).…”

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confidence: 99%

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures

et al. 2015

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Enhanced Luminescence properties of mononuclear lanthanide complexes with asymmetric seven-coordination structure are reported for the first time. The lanthanide complexes are composed of a lanthanide ion (Eu III or Tb III ), three tetramethyl heptanedionatos and one triphenyl phosphine oxide. The coordination geometries of the lanthanide complexes are evaluated using single crystal X-ray analyses and shape-measurement calculations. The complexes are categorized to be seven-coordinate monocapped octahedral structure (point group: C3v). The sevencoordinate lanthanide complexes show high intrinsic emission quantum yields, extra-large radiative rate constants and unexpected small non-radiative rate constants. The brilliant luminescence properties are elucidated in terms of the asymmetric coordination geometry and small vibrational quanta related to the thermal relaxation.

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Section: Introductionmentioning

confidence: 99%

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures

et al. 2015

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“…25 However, the procedure has one key requirement; the probes must have constant yet signicantly different decay times. Here, we chose neodymium and ytterbium based probes.…”

Section: 48mentioning

confidence: 99%

“…25 The background emission has a nanosecond luminescence decay time and can be imaged and removed using simple time gating. Fig.…”

Section: 25mentioning

confidence: 99%

“…46,47 We have recently shown that it is possible to resolve lanthanide luminescence spectrally in order to achieve background free energy-resolved images. 25 We now demonstrate a variation on the two time-resolved imaging methods mentioned above, which we have named photon arrival time imaging or PArTI. The method allows us to obtain resolved images by using the differences in emission decay time by applying a procedure where photons in specic arrival time intervals are assigned a colour and then integrated to provide a probe specic contrast.…”

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Time-resolved confocal microscopy using lanthanide centred near-IR emission

et al. 2015

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A method to uniquely identify signals originating from probes with different emission decay times in luminescence imaging has been developed. By using scanning confocal microscopy in combination with time-correlated single photon counting (TCSPC), Photon Arrival Time Imaging (PArTI) has been realised through off-line plotting of images using the photon arrival times. PArTI is the time-equivalent to spectrally resolved imaging, replacing the energy axis with a photon arrival time axis. Here, lanthanide probes were used to demonstrate the key advantages of the method. PArTI uses TCSPC data, involves no fitting, uses a single pulsed laser line for multicolour imaging, and can be used with a 100 millisecond dwell time per pixel.In cells and tissue, autouorescence and scattering remain an issue that can limit the sensitivity of imaging microscopy. 1,2Time-resolved microscopy and time-gated microscopy are ideally suited to remove these contributions.3-13 Fluorescence/ phosphorescence lifetime imaging (FLIM/PLIM) measures and creates an image of the luminescence decay time of a given molecular probe by tting the emission decay prole for each pixel, 3,[6][7][8][13][14][15][16][17][18] while time-gated microscopy exploits molecular probes with a decay time signicantly longer than the background in order to electronically or mechanically gate the light and thus completely remove background emission without tting the emission decay curve. [3][4][5][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] In some applications, multiple hardware time-gates in combination with a tting routine have been used previously.38 With lanthanide based probes, time-gated microscopy has been used extensively, 3,4,[19][20][21][22] and examples of FLIM/PLIM images have also been published. 21Lanthanide luminescence is unique as it is well-dened in energy, when compared to transition metals or organic emitters.39-45 Lanthanide centred luminescence is structured in narrow emission bands and a lanthanide complex will have a characteristic, although medium specic, luminescence decay time (s).46,47 We have recently shown that it is possible to resolve lanthanide luminescence spectrally in order to achieve background free energy-resolved images. 25We now demonstrate a variation on the two time-resolved imaging methods mentioned above, which we have named photon arrival time imaging or PArTI. The method allows us to obtain resolved images by using the differences in emission decay time by applying a procedure where photons in specic arrival time intervals are assigned a colour and then integrated to provide a probe specic contrast. In the example presented here, lanthanide centred luminescence with two different decay times and emission in the near-IR (NIR) was used, though further multiplexing of the technique is readily achievable.PArTI is explained in detail in Fig. 1, which shows how signals from different probes can be separated on the basis of their emissive lifetimes, since the contribution from each probe wil...

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“…For the first time, it has been possible to visualize and to count nucelosomes, which, packaged together, form our genome, thanks to super-resolution microscopy [1].…”

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confidence: 99%

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TrAC Trends in Analytical Chemistry

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Spectrally resolved confocal microscopy using lanthanide centred near-IR emission

Cited by 37 publications

References 26 publications

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures

Time-resolved confocal microscopy using lanthanide centred near-IR emission

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Spectrally resolved confocal microscopy using lanthanide centred near-IR emission

Cited by 37 publications

References 26 publications

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C3v Geometrical Structures

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C3v Geometrical Structures

Time-resolved confocal microscopy using lanthanide centred near-IR emission

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Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures

Seven‐Coordinate Luminophores: Brilliant Luminescence of Lanthanide Complexes with C_3v Geometrical Structures