Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panel

Tu, Dandan; Holderby, Allison; Guo, Heng; Mabbott, Samuel; Tian, Limei; Coté, Gerard L.

doi:10.1016/j.aca.2022.339562

Cited by 13 publications

(11 citation statements)

References 47 publications

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“…In theory, by changing the detection antibodies or using other recognition molecules (e.g., peptides, aptamers, and antibiotics) for bacteria, the proposed Mag@Au SERS-ICA has the potential to detect more types of pathogens with high sensitivity and selectivity. Notably, some recent researches show that the combination of different Raman-reporter-molecule-modified SERS tags and a single test line can greatly improve the multiplex detection ability of the ICA strip and simplifies the measurement process of SERS signals. − In the future, we will integrate different Raman-reporter-molecule-modified Mag@Au tags into a single test-line-based ICA strip to further broaden the application potential of the SERS-ICA technique.…”

Section: Resultsmentioning

confidence: 99%

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Wang

et al. 2022

ACS Appl. Nano Mater.

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Herein, we present an ultrasensitive surfaceenhanced Raman scattering (SERS)-based immunochromatographic assay (ICA) for the multiplex detection of foodborne bacteria in food samples by using nanogapped Fe 3 O 4 @Au (Mag@ Au) as multifunctional SERS tags. The Mag@Au tags with multiple surface hotspots were integrated into the ICA system for the first time to provide a superior SERS signal for ICA analysis, high stability for complex samples, and magnetic enrichment ability for target pathogens. Visible test lines were successfully introduced by mixing bovine serum albumin (BSA)-coated gold nanoparticles (Au@BSA) with detection antibodies, which can facilitate the accurate positioning of test zones for quantitative SERS analysis. The magnetic SERS-ICA can achieve simultaneous and quantitative detection of Salmonella typhimurium and Staphylococcus aureus in 30 min with low limits of detection of 12 and 9 cells mL −1 , respectively. The proposed method further demonstrated good stability and accuracy in various food samples and thus possesses great potential for on-site and sensitive monitoring of bacteria.

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Section: Resultsmentioning

confidence: 99%

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Wang

et al. 2022

ACS Appl. Nano Mater.

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“…Nanoparticles such as AuNPs, graphene, and carbon dots are critical protagonists in different types of biosensors to enable ultra-sensitive and multiple detection of cardiac biomarkers, including myoglobin, cTnI, cTnT, CK-MB, H-FABP, exosomes, and miRNAs. 241 – 243 …”

Section: Nanoparticle-based Diagnostic Strategies For Vascular Aging-...mentioning

confidence: 99%

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases

Liu

2022

Sig Transduct Target Ther

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Aging-induced alternations of vasculature structures, phenotypes, and functions are key in the occurrence and development of vascular aging-related diseases. Multiple molecular and cellular events, such as oxidative stress, mitochondrial dysfunction, vascular inflammation, cellular senescence, and epigenetic alterations are highly associated with vascular aging physiopathology. Advances in nanoparticles and nanotechnology, which can realize sensitive diagnostic modalities, efficient medical treatment, and better prognosis as well as less adverse effects on non-target tissues, provide an amazing window in the field of vascular aging and related diseases. Throughout this review, we presented current knowledge on classification of nanoparticles and the relationship between vascular aging and related diseases. Importantly, we comprehensively summarized the potential of nanoparticles-based diagnostic and therapeutic techniques in vascular aging and related diseases, including cardiovascular diseases, cerebrovascular diseases, as well as chronic kidney diseases, and discussed the advantages and limitations of their clinical applications.

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“…The SERS-based paper fluidic cartridges were made using a previously developed method, which showed high detection sensitivity in the clinical range of interest for cTnI and HFABP detection. 57 The detection range, concentration versus signal output linearity, the limit of detection (LOD), and signal-to-background ratios (SBRs) were compared. The impact of laser power on detection performance was also characterized.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Clinical Utility of Raman Spectroscopy for Point-of-Care Cardiovascular Disease Biomarker Detection

Soliman

Mabbott

et al. 2023

Appl Spectrosc

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A variety of innovative point-of-care (POC) solutions using Raman systems have been explored. However, the vast effort is in assay development, while studies of the characteristics required for Raman spectrometers to function in POC applications are lacking. In this study, we tested and compared the performance of eight commercial Raman spectrometers ranging in size from benchtop Raman microscopes to portable and handheld Raman spectrometers using paper fluidic cartridges, including their ability to detect cardiac troponin I and heart fatty acid binding protein, both of which are well-established biomarkers for evaluating cardiovascular health. Each spectrometer was evaluated in terms of excitation wavelength, laser characteristics, and ease of use to investigate POC utility. We found that the Raman spectrometers equipped with 780 and 785 nm laser sources exhibited a reduced background signal and provided higher sensitivity compared to those with 633 and 638 nm laser sources. Furthermore, the spectrometer equipped with the single acquisition line readout functionality showed improved performance when compared to the point scan spectrometers and allowed measurements to be made faster and easier. The portable and handheld spectrometers also showed similar detection sensitivity to the gold standard instrument. Lastly, we reduced the laser power for the spectrometer with single acquisition line readout capability to explore the system performance at a laser power that change the classification from a Class 3B laser device to a Class 3R device and found that it showed comparable performance. Overall, these findings show that portable Raman spectrometers have the potential to be used in POC settings with accuracy comparable to laboratory-grade instruments, are relatively low-cost, provide fast signal readout, are easy to use, and can facilitate access for underserved communities.

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Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panel

Cited by 13 publications

References 47 publications

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases

Exploring the Clinical Utility of Raman Spectroscopy for Point-of-Care Cardiovascular Disease Biomarker Detection

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Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panel

Cited by 13 publications

References 47 publications

Nanogapped Fe3O4@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Nanogapped Fe3O4@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases

Exploring the Clinical Utility of Raman Spectroscopy for Point-of-Care Cardiovascular Disease Biomarker Detection

Contact Info

Product

Resources

About

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

Nanogapped Fe₃O₄@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip