Spectral Shaping of Artificial UV-B Irradiation for Vegetation Stress Research

Döhring, Thorsten; Köfferlein, Matthias; Thiel, Stephan; Seidlitz, Harald K.

doi:10.1016/s0176-1617(96)80302-6

Cited by 50 publications

(27 citation statements)

References 5 publications

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“…The short-wave cut-off was achieved by selected soda lime and acrylic glass filters. Detailed descriptions of the sun simulator facility were given by Döhring et al (1996) and Thiel et al (1996). The experimental period was 74 h. The radiation period lasted for 16 h per day, with 400 μmol m −2 s −1 PAR (400–700 nm) plus UV-A (315–400 nm)—this mimics the natural situation, where PAR is always combined with UV-A (designated as PA); UV-B radiation (280–315 nm) was added 1 h after the start of illumination and switched off 1 h before the dark phase, providing a total UV-B exposure of 14 h per day (designated as PAB).…”

Section: Methodsmentioning

confidence: 99%

Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

et al. 2018

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Species of Zygnema form macroscopically visible mats in polar and temperate terrestrial habitats, where they are exposed to environmental stresses. Three previously characterized isolates (Arctic Zygnema sp. B, Antarctic Zygnema sp. C, and temperate Zygnema sp. S) were tested for their tolerance to experimental UV radiation. Samples of young vegetative cells (1 month old) and pre-akinetes (6 months old) were exposed to photosynthetically active radiation (PAR, 400–700 nm, 400 μmol photons m−2 s−1) in combination with experimental UV-A (315–400 nm, 5.7 W m−2, no UV-B), designated as PA, or UV-A (10.1 W m−2) + UV-B (280–315 nm, 1.0 W m−2), designated as PAB. The experimental period lasted for 74 h; the radiation period was 16 h PAR/UV-A per day, or with additional UV-B for 14 h per day. The effective quantum yield, generally lower in pre-akinetes, was mostly reduced during the UV treatment, and recovery was significantly higher in young vegetative cells vs. pre-akinetes during the experiment. Analysis of the deepoxidation state of the xanthophyll-cycle pigments revealed a statistically significant (p < 0.05) increase in Zygnema spp. C and S. The content of UV-absorbing phenolic compounds was significantly higher (p < 0.05) in young vegetative cells compared to pre-akinetes. In young vegetative Zygnema sp. S, these phenolic compounds significantly increased (p < 0.05) upon PA and PAB. Transmission electron microscopy showed an intact ultrastructure with massive starch accumulations at the pyrenoids under PA and PAB. A possible increase in electron-dense bodies in PAB-treated cells and the occurrence of cubic membranes in the chloroplasts are likely protection strategies. Metabolite profiling by non-targeted RP-UHPLC-qToF-MS allowed a clear separation of the strains, but could not detect changes due to the PA and PAB treatments. Six hundred seventeen distinct molecular masses were detected, of which around 200 could be annotated from databases. These results indicate that young vegetative cells can adapt better to the experimental UV-B stress than pre-akinetes.Electronic supplementary materialThe online version of this article (10.1007/s00709-018-1225-1) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

et al. 2018

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“…The lamp types are arranged in several groups to realize the natural variations of the solar irradiance by switching appropriate group of lamps on and oV. The short-wave cut-oV was shaped by selected borosilicate and soda-lime glass Wlters (Döhring et al 1996). The plants were exposed to radiation for 16 h per day in the sun simulators at climate conditions of 24°C temperature and 70% relative humidity.…”

Section: Uv-b Exposurementioning

confidence: 99%

The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana

Meissner

Albert

Böttcher

et al. 2008

Planta

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Arabidopsis harbors four UDP-glycosyltransferases that convert hydroxycinnamates (HCAs) to 1-O-beta-glucose esters, UGT84A1 (encoded by At4g15480), UGT84A2 (At3g21560), UGT84A3 (At4g15490), and UGT84A4 (At4g15500). To elucidate the role of the individual UGT84A enzymes in planta we analyzed gene expression, UGT activities and accumulation of phenylpropanoids in Arabidopsis wild type plants, ugt mutants and overexpressing lines. Individual ugt84A null alleles did not significantly reduce the gross metabolic flux to the accumulating compounds sinapoylcholine (sinapine) in seeds and sinapoylmalate in leaves. For the ugt84A2 mutant, LC/MS analysis revealed minor qualitative and quantitative changes of several HCA choline esters and of disinapoylspermidine in seeds. Overexpression of individual UGT84A genes caused increased enzyme activities but failed to produce significant changes in the pattern of accumulating HCA esters. For UGT84A3, our data tentatively suggest an impact on cell wall-associated 4-coumarate. Exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and led to a transient increase in sinapoylglucose and sinapoylmalate concentrations.

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“…CA removes UV-C, and polyester (Mylar) removes most of the UV-B radiation. The removal of the extraneous UV-C by the use of CA or glass filters attempts to simulate the wavelength distribution of solar radiation at ground level (see discussion in Döhring et al [8]). The CA and Mylar transmissions confirm the well-established presence of UV-A in the SWB protocol for filtering UV-B lamps (4,5; Table 1, Fig.…”

Section: Uv-blocking Filter Spectra and Emission Fluxmentioning

confidence: 99%

“…The control exposure generally uses polyester (Mylar) to exclude UV-B from UV-A radiation sources (although a few studies imply that no such control was used). Glass or rigid plastic SWB filters are sometimes used instead of CA and Mylar (6)(7)(8)(9). This widely used protocol (termed the SWB protocol) assumes that by subtracting physiological effects attributed to UV-A exposure (i.e.…”

Section: Introductionmentioning

confidence: 99%

Removing UV-A and UV-C Radiation from UV-B Fluorescent Lamp Emissions. Differences in the Inhibition of Photosynthesis in the Marine Alga Dunaliella tertiolecta Using Chromate Versus Cellulose Acetate–Polyester Filters

White

Jahnke

2004

Photochem Photobiol

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Ultraviolet-B (UV-B; 280-320 nm)-emitting lamps unavoidably emit ultraviolet-A (UV-A; 320-400 nm) and ultraviolet-C (UV-C; <280 nm) radiation. Short-wavelength-blocking filters are generally used to limit the wave bands of UV under investigation. The widespread use of such filters means that all exposures to UV-B radiation will have a significant UV-A component. Therefore, the physiological effects unique to UV-B exposure are difficult to clearly isolate. This study presents a method to remove the UV-A and UV-C "contamination" using a liquid potassium chromate (K(2)CrO(4)) filter, thus allowing more direct assessment of the effects of UV-B exposure. Cultures of the green marine alga Dunaliella tertiolecta were grown in the absence of UV radiation. Sunlamps supplied the UV radiation for a 24 h exposure (solar radiation was not used in this study). The UV radiation was filtered either by the standard method (i.e. cellulose acetate (CA) with polyester = Mylar controls) or by a liquid filter of potassium chromate. Photosynthetic responses were compared. Major decreases in the ratio of variable to maximal fluorescence in dark-adapted cells and photosynthetic capacity were observed in CA-filtered cultures, whereas no change was observed in cells exposed to the same UV-B flux with the UV-A removed by K(2)CrO(4). The use of a CA filter with a Mylar control does not link results unequivocally to UV-B radiation. Such results should be interpreted with caution.

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Spectral Shaping of Artificial UV-B Irradiation for Vegetation Stress Research

Cited by 50 publications

References 5 publications

Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

Arctic, Antarctic, and temperate green algae Zygnema spp. under UV-B stress: vegetative cells perform better than pre-akinetes

The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana

Removing UV-A and UV-C Radiation from UV-B Fluorescent Lamp Emissions. Differences in the Inhibition of Photosynthesis in the Marine Alga Dunaliella tertiolecta Using Chromate Versus Cellulose Acetate–Polyester Filters

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