Spectral sensitivity of light induced respiratory activity of photoreceptor mitochondria in the intact fly

Tinbergen, Joost M.; Stavenga, Doekele G.

doi:10.1007/bf00609726

Cited by 17 publications

(10 citation statements)

References 38 publications

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“…Action spectra for both activation and adaptation of the blue, light-induced green fluorescence measured on eyes of intact flies (Fig. 16) agree with the sensitivity spectrum of photoreceptors R1-6 (Tinbergen and Stavenga, 1987), reinforcing the conclusion that activation of the phototransduction process boosts mitochondrial activity, i.e. ATP production.…”

Section: Flavoproteins and Nadhsupporting

confidence: 73%

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Insect retinal pigments: Spectral characteristics and physiological functions

Stavenga

1995

Progress in Retinal and Eye Research

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Section: Flavoproteins and Nadhsupporting

confidence: 73%

“…For the adaptation spectrum, a similar criterion was set for a long adaptation light of variable wavelength. The dotted line represents the rhodopsin absorbance spectrum (from Tinbergen and Stavenga, 1987).…”

Section: Imentioning

confidence: 99%

Insect retinal pigments: Spectral characteristics and physiological functions

Stavenga

1995

Progress in Retinal and Eye Research

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“…Changes in the blue-induced green fluorescence reflect changes in the redox state of the mitochondrial flavoproteins, thus indicating changes in the oxidative metabolism (Tinbergen and Stavenga 1986). The action spectrum of a criterion change in the blue-induced green fluorescence closely resembles the absorption spectrum of the visual pigment as well as the spectral sensitivity measured electrophysiologically, indicating that the fluorescence change is linked to the visual transduction pathway (Tinbergen and Stavenga 1987).…”

Section: Introductionmentioning

confidence: 79%

“…Nevertheless, we are able to determine the intensity dependence of the mitochondrial activation by using the approach of Fig. 2, where the mitochondrial activation elicited by a flash of variable intensity is tested by a subsequent bright blue illumination (see also Tinbergen and Stavenga 1987).…”

Section: Light Intensity Dependence Of Mitochondrial Activationmentioning

confidence: 99%

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Receptor potential and light-induced mitochondrial activation in blowfly photoreceptor mutants

1991

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Summary. 1. Simultaneous measurements of the receptor potential and the light-induced mitochondrial activation were performed in white-eyed blowflies Calliphora vicina, mutant chalky, and Lucilia cuprina, mutants w r and w'nss. The intensity dependence and the temporal dynamics were investigated.2. The characteristic curve of the light-induced mitochondrial activation vs. log intensity has an S-like shape, which is much steeper than the characteristic curve of the receptor potential (the V/log I curve). The threshold intensity of mitochondrial activation elicits about a half-maximal receptor potential, while mitochondrial activation and photoreceptor potential saturate at about the same intensity.3. The time course of the mitochondrial activation induced by a light flash, i.e. the pulse-response curve, is biphasic in both mutants with normal phototransduction properties, Calliphora chalky and Lucilia wF ; the shape is slightly species dependent.4. In the phototransduction mutant Lucilia w'nss the step-responses of both the (bright) light-induced mitochondrial activation and receptor potential are quite different from the corresponding signals in Lucilia w F. The striking resemblance of the step-response of the mitochondrial activation to its pulse-response indicates that mitochondrial activation and receptor potential are intimately linked.

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Mitochondria are redistributed in Drosophila photoreceptors lacking Milton, a kinesin‐associated protein

Górska-Andrzejak

Stowers

Borycz

et al. 2003

J of Comparative Neurology

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Photoreceptors are richly supplied with mitochondria, where they are required to meet the energetic demands, in the soma, of phototransduction and, in the terminal, of neurotransmitter release. Compromising the latter, we have made photoreceptors R1-R6 in Drosophila ommatidia homozygous for either of two alleles, milt(186) and milt(92), of milton in whole-eye mosaics. Such mutant photoreceptors fail to target mitochondria to their terminals. We show from quantitative electron microscopy (EM) that mitochondria are totally lacking at the terminal but nevertheless abundant and present throughout the soma, where their distribution differs from that of control ommatidia, however, being more heavily concentrated in the nuclear region. Mitochondria are sparse at the basalmost level of mutant ommatidia, and are lacking beneath the basement membrane, in the axons and terminals of these cells. The absence of mitochondria from R1-R6 terminals and concommitant reductions in synaptic vesicle packing density, previously reported, we show here are accompanied by reduced immunoreactivity to the photoreceptor transmitter histamine but not by any change in total head histamine content, as determined by high-performance liquid chromatography. Mutant terminals also contain vesicle profiles with a wider range of sizes. These two phenotypes suggest that the reduced availability of ATP when mutant terminals lack a mitochondrial supply compromises their ability to pump histamine into synaptic vesicles and perturbs membrane distribution within the terminal. In addition, a band of somata in the lamina cortex, at least some of which are postsynaptic neurons not homozygous for milton, also shows altered mitochondrial targeting, with abnormal clusters of mitochondria, as visualized by immunolabeling with anti-hsp and by serial EM. Within the lamina, terminals of mutant photoreceptors are penetrated by neighboring cells with invaginations that frequently contain mitochondria, suggesting that a mechanism exists for intercellular metabolic support. Our findings indicate the direct and compensatory responses in a population of neurons when mitochondria are not correctly targeted to their synaptic terminals.

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Spectral sensitivity of light induced respiratory activity of photoreceptor mitochondria in the intact fly

Cited by 17 publications

References 38 publications

Insect retinal pigments: Spectral characteristics and physiological functions

Insect retinal pigments: Spectral characteristics and physiological functions

Receptor potential and light-induced mitochondrial activation in blowfly photoreceptor mutants

Mitochondria are redistributed in Drosophila photoreceptors lacking Milton, a kinesin‐associated protein

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