Spectral Reflectance Indexes Reveal Differences in the Physiological Status of Brassica oleracea with Contrasting Glucosinolate Content under Biotic Stress

Abstract

Brassica species produce glucosinolates, a specific group of secondary metabolites present in the Brassicaceae family with antibacterial and antifungal properties. The employment of improved varieties for specific glucosinolates would reduce the production losses caused by pathogen attack. However, the consequences of the increment in these secondary metabolites in the plant are unknown. In this work, we utilized reflectance indexes to test how the physiological status of Brasica oleracea plants changes depending on their constitutive content of glucosinolates under nonstressful conditions and under the attack of the bacteria Xanthomonas campestris pv. campestris and the fungus Sclerotinia sclerotiorum. The modification in the content of glucosinolates had consequences in the resistance to both necrotrophic pathogens, and in several physiological aspects of the plants. By increasing the content in sinigrin and glucobrassicin, plants decrease photosynthesis efficiency (PR531, FvFm), biomass production (CHL-NDVI, SR), pigment content (SIPI, NPQI, RE), and senescence (YI) and increase their water content (WI900). These variables may have a negative impact in the productivity of crops in an agricultural environment. However, when plants are subjected to the attack of both necrotrophic pathogens, an increment of sinigrin and glucobrassicin confers an adaptative advantage to the plants, which compensates for the decay of physiological parameters.