Spectral Photoluminescent Parameters of Barley Seeds (Hordéum vulgáre) Infected with Fusarium ssp

Abstract

We needed effective and sustainable technologies for better microbiological control of crops, including Fusarium. However, photoluminescent UV-Vis methods are potential for diagnosing plant diseases with Fusarium. It has not been sufficiently studied despite the application of these methods for other biological researches. The excitation spectrum of the seeds during infection shifts to the shorter wavelength and a new maximum appears in the region λ ≈ 232 nm. The photoluminescence of infected seeds increases with excitation by radiation of wavelengths λ e,1 = 232 nm, λ e,2 = 362 nm and λ e,3 = 424 nm by 1.33-3.14 times, and λ e,3 = 424 nm-decreases by 1.1 times. Statistical moments μ 3 and μ 4 , asymmetry and kurtosis change only with short-wave excitation. When analyzing the decomposition of the frequency spectrum into Gaussian curves, the most informative ratio is the ratio of right-handed and left-handed Gaussians under excitation λ e,2 = 362 nm and λ e,3 = 424 nm. The ratios of their maxima change during infection by 1.36-3.2 times, and for excitation by radiation λ e,2 , the frequency boundaries of Gaussians change. The results of measurements and calculations provide a basis for the development of a method and device for photoluminescence diagnostics of fusarium seeds in UV-Vis ranges.

Classification of Plenodomus lingam and Plenodomus biglobosus in Co-Occurring Samples Using Reflectance Spectroscopy

“…Photographs of the Petri dishes with the fungal mycelia were taken on eight dates (1,3,8,10,15,17,22, and 24 days after inoculation) with a Canon 80D SLR camera (Canon, London, Great Britain) with a varifocal lens F-S 18-135mm f/3.5-5.6 IS USM. Images were taken at 85 mm focal length, 5.6 aperture, ISO 1600, and variable auto-adjustable shutter speed (the only variable shooting parameter).…”

“…Remote sensing techniques have recently been widely used to detect crop pathogens [1][2][3]. The successful application of these methods in field conditions was usually preceded by tests carried out under controlled conditions in the laboratory [4][5][6][7][8].…”

Determination of the Dependences of the Nutritional Value of Corn Silage and Photoluminescent Properties

“…Moreover, photoluminescence has been actively utilized in the industrial processing of food products and the preparation of semi-finished goods. For instance, in the range of 550 nm, myoglobin in poultry meat actively fluoresces, allowing the degree of poultry cooking to be determined through its detection [16].…”