Spectral–luminescent properties of laurdan molecule

Titova, T. Yu.; Artyukhov, V. Ya.; Zharkova, O. M.; Morozova, Julia P.

doi:10.1016/j.saa.2013.12.097

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…The inset in Figure 8 shows as obtained from Laurdan as a function of T*, using = 10 . −1 , where R is the gas constant and Γ 0 ≈ 50 approximated as FWHM of Laurdan in n-hexane (67). The estimated specific heat profile is remarkably similar to quantum statistical calculated previously (35).…”

Section: +1supporting

confidence: 83%

On measuring the acoustic state changes in lipid membranes using fluorescent probes

2018

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Ultrasound is increasingly being used to modulate the properties of biological membranes for applications in drug delivery and neuromodulation. While various studies have investigated the mechanical aspect of the interaction such as acoustic absorption and membrane deformation, it is not clear how these effects transduce into biological functions, for example, changes in the permeability or the enzymatic activity of the membrane. A critical aspect of the activity of an enzyme is the thermal fluctuations of its solvation or hydration shell. Thermal fluctuations are also known to be directly related to membrane permeability. Here solvation shell changes of lipid membranes subject to an acoustic impulse were investigated using a fluorescence probe, Laurdan.Laurdan was embedded in multi-lamellar lipid vesicles in water, which were exposed to broadband pressure impulses of the order of 1MPa peak amplitude and 10μs pulse duration. An instrument was developed to monitor changes in the emission spectrum of the dye at two wavelengths with sub-microsecond temporal resolution. The experiments show that changes in the emission spectrum, and hence the fluctuations of the solvation shell, are related to the changes in the thermodynamic state of the membrane and correlated with the compression and rarefaction of the incident sound wave. The results suggest that acoustic fields affect the state of a lipid membrane and therefore can potentially modulate the kinetics of channels and proteins embedded in the membrane.

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Section: +1supporting

confidence: 83%

On measuring the acoustic state changes in lipid membranes using fluorescent probes

2018

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“…Specifically, this effect was found by the measurements of the generalized polarization of Laurdan (2-dimethylamino-6-dodecanoylnaphthalene) in micelles composed of anionic gangliosides G1, G2, G3, and model membranes, containing dipalmitoylphosphatidylcholine (DPPC) and its mixtures with CL [41,42]. Laurdan and Prodan (2-dimethylamino-6-propionylnaphthalene) molecules possess donor and acceptor moieties, high ground and excited state dipole moments, which are also the characteristic features of benzanthrones [22,43]. The two emission bands of the dyes are associated with a pair of non-relaxed (or locally excited) and solvent-relaxed (or twisted internal charge transfer) S1 states, the latter being predominant in polar solvents.…”

Section: Association Of Benzanthrone Dyes With Model Lipid Membranesmentioning

confidence: 94%

Novel benzanthrone probes for membrane and protein studies

Ryzhova

Vus

Trusova

et al. 2016

Methods Appl. Fluoresc.

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The applicability of a series of novel benzanthrone dyes to monitoring the changes in physicochemical properties of lipid bilayer and to differentiating between the native and aggregated protein states has been evaluated. Based on the quantitative parameters of the dye-membrane and dye-protein binding derived from the fluorimetric titration data, the most prospective membrane probes and amyloid tracers have been selected from the group of examined compounds. Analysis of the red edge excitation shifts of the membrane- and amyloid-bound dyes provided information on the properties of benzanthrone binding sites within the lipid and protein matrixes. To understand how amyloid specificity of benzanthrones correlates with their structure, quantitative structure activity relationship (QSAR) analysis was performed involving a range of quantum chemical molecular descriptors. A statistically significant model was obtained for predicting the sensitivity of novel benzanthrone dyes to amyloid fibrils.

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“…Fluorescence spectroscopy and microscopy, in particular, are today key techniques for studying cellular membrane properties and processes. , Central to these experimental techniques is the use of fluorescent dyes that function as probes. The optical properties of the probes are affected by their surrounding environment and thus report about the composition and properties of a lipid environment. − Developing such probes can be challenging since the probe should possess a number of specific properties in order to be considered suitable. − First of all, the probe must show great fluorescence properties, i.e., it must possess excitation and emission energies well-separated (for good Stokes shifts) and at low energies in order to prevent cell damage. Additionally, the probes should have good brightness and photostability. , From a structural point of view, the probe should perturb the membrane only marginally and target the membrane of interest with high specificity…”

Section: Introductionmentioning

confidence: 99%

Computational Characterization of Novel Malononitrile Variants of Laurdan with Improved Photophysical Properties for Sensing in Membranes

et al. 2020

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Fluorescent probes are powerful tools for improving our understanding of cellular membranes and other complex biological environments. Using simulations, we gain atomistic and electronic insights into the effectiveness of the probes. In the current work, we have used various computational approaches to comprehensively investigate the properties of the fluorescent probe Laurdan and two Laurdan-like probes: AADAL and ECL. In addition, we propose the development of their corresponding novel malononitrile variants, which are computationally characterized herein. For the candidate probes, electronic structure calculations were used to rationalize their optical properties, including their ability for two-photon activation, and molecular dynamics simulations were used to unravel atomistic details of their functioning within lipid bilayers. While Laurdan, AADAL, and ECL were found to have very similar optical and membrane partitioning profiles, their malononitrile variants were found to show significantly improved optical properties, especially in regard to two-photon cross sections, and they appear to retain the desired membrane characteristics of the parent Laurdan molecule.

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Spectral–luminescent properties of laurdan molecule

Cited by 7 publications

References 21 publications

On measuring the acoustic state changes in lipid membranes using fluorescent probes

On measuring the acoustic state changes in lipid membranes using fluorescent probes

Novel benzanthrone probes for membrane and protein studies

Computational Characterization of Novel Malononitrile Variants of Laurdan with Improved Photophysical Properties for Sensing in Membranes

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