Spectral fingerprinting revealed modulation of plant secondary metabolites in providing abiotic stress tolerance to invasive alien plants Lantana camara (L.), Parthenium hysterophorous (L.), Ricinus communis (L.), and Ageratum conyzoides (L.) (plant metabolites in stress tolerance to invasive plants)

Sharma, Arun Dev; Kaur, Jasmeet; Chand, Thakur

doi:10.5114/bta.2021.108727

Cited by 2 publications

(1 citation statement)

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“…As an important secondary metabolite, rutin is not only of great benefit to human health, but also has an important role for the buckwheat plant itself. For instance, rutin can protect the plant from solar UV radiation, dryness, cold, salinity, and a range of pests (Gaberscik et al ., 2002 ; Ismail et al ., 2015 ; Kreft et al ., 2002 ; Sharma et al ., 2021 ; Suzuki et al ., 2005 ; Yang et al ., 2022 ; Zhao et al ., 2021a ). However, rutin in buckwheat is unstable and its accumulation is highly susceptible to environmental influences such as altitude, light, moisture, and other environmental factors (Hao et al ., 2016 ; Suzuki et al ., 2005 ; Tsurunaga et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Multi‐omics identification of a key glycosyl hydrolase gene FtGH1 involved in rutin hydrolysis in Tartary buckwheat (Fagopyrum tataricum)

Lai,

Zhang,

et al. 2023

Plant Biotechnology Journal

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SummaryRutin, a flavonoid rich in buckwheat, is important for human health and plant resistance to external stresses. The hydrolysis of rutin to quercetin underlies the bitter taste of Tartary buckwheat. In order to identify rutin hydrolysis genes, a 200 genotypes mini‐core Tartary buckwheat germplasm resource was re‐sequenced with 30‐fold coverage depth. By combining the content of the intermediate metabolites of rutin metabolism with genome resequencing data, metabolite genome‐wide association analyses (GWAS) eventually identified a glycosyl hydrolase gene FtGH1, which could hydrolyse rutin to quercetin. This function was validated both in Tartary buckwheat overexpression hairy roots and in vitro enzyme activity assays. Mutation of the two key active sites, which were determined by molecular docking and experimentally verified via overexpression in hairy roots and transient expression in tobacco leaves, exhibited abnormal subcellular localization, suggesting functional changes. Sequence analysis revealed that mutation of the FtGH1 promoter in accessions of two haplotypes might be necessary for enzymatic activity. Co‐expression analysis and GWAS revealed that FtbHLH165 not only repressed FtGH1 expression, but also increased seed length. This work reveals a potential mechanism behind rutin metabolism, which should provide both theoretical support in the study of flavonoid metabolism and in the molecular breeding of Tartary buckwheat.

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