Spectral Distribution of the Light Scattered from Rodlike Macromolecules in Solution

Maeda, Hideatsu; Saitô, Nobuhiko

doi:10.1143/jpsj.27.984

Cited by 49 publications

(14 citation statements)

References 6 publications

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“…52,[67][68][69][70][71] Since the basis of the current analysis is a linear Langevin equation, this coupling is lost. The question, how severely the dynamic structure factor of the semiflexible polymer-and hence the FCS correlation function-is affected by this approximation remains to be clarified.…”

Section: ͑49͒mentioning

confidence: 99%

Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy

Winkler

2007

The Journal of Chemical Physics

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The dynamics of weakly bending polymers is analyzed on the basis of a Gaussian semiflexible chain model and the fluorescence correlation spectroscopy (FCS) correlation function is determined. Particular attention is paid to the influence of the rotational motion on the decay of the FCS correlation function. An analytical expression for the correlation function is derived, from which the averaged segmental mean square displacement can be determined independent of any specific model for the polymer dynamics. The theoretical analysis exhibits a strong dependence of the correlation function on the rotational motion for semiflexible polymers with typical lengths and persistence lengths of actin filaments or fd viruses. Hence, FCS allows for a measurement of the rotational motion of such semiflexible polymers. The theoretical results agree well with experimental measurements on actin filaments and confirm the importance of large relaxation times.

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Section: ͑49͒mentioning

confidence: 99%

Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy

Winkler

2007

The Journal of Chemical Physics

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“…12), rigid rods (Refs. [13][14][15], hinged rods (Ref. 16) and Gaussian chains with pre-averaged hydrodynamic interaction (Refs.…”

Section: Translational Diffusionmentioning

confidence: 99%

Effects of polydispersity, branching and chain stiffness on quasielastic light scattering

Stockmayer¹,

Schmidt²

1982

Pure and Applied Chemistry

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-Aspects of polarized coherent quasi-elastic scattering by dilute macromolecular solutions are discussed. Effects of polydispersity, branching and chain stiffness are considered, particularly in respect to the observable first cumulant of the dynamic structure factor and its dependence on the scattering angle. A combination of integrated and time-resolved scattering data yields the maximum information.

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“…Detailed theory of light-scattering spectra for such systems is virtually nonexistent at the present time. However, Pecora 3 D-33 and Saito et al [34][35][36] have calculated the light scattering spectra of rigid rodlike molecules and of flexible coils.…”

Section: A Brief Theoretical Reviewmentioning

confidence: 99%

Dyamics of calf-thymus DNA by single-clipped photon correlation

Chen

Yeh

Chu

1977

The Journal of Chemical Physics

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Articles you may be interested inSynthesis and characterisation of platinum (II) salphen complex and its interaction with calf thymus DNA AIP Conf.Interaction of Bis-Zn(II) salphen complex with calf thymus-DNA AIP Conf.Time-dependent correlation functions of calf-thymus DNA (Mw = l2X 10 6 dalton) and homogenized calfthymus DNA (Mw = 1.5 X 10 6 dalton) were measured by means of a 96-channel single-clipped digital photon correlation spectrometer over a range of scattering angles varying from IS' to 120'. Several models as well as the method of cumulants were used to analyze our experimental data which were found to contain at least two decay times. While we agree with earlier findings on the complexity of the timedependent correlation functions for DNA, our results have led us to different conclusions. For example, we could not find the "longest relaxation time." Instead, we observed a simple K 2 dependence on the linewidth (r) of the low-frequency component of time-dependent correlation functions at small scattering angles, where K is the magnitude of the momentum transfer vector. Furthermore, we were able to identify those low-frequency component (r I K 2) values with the translational self-diffusion coefficient which had been determined by independent light scattering and ultracentrifugal studies.Eq. (1. 1), they obtained r 2 = DK2 for the high-frequency component, indicating that 6.-l ""0 rather than a finite value as reported by Schmidt. 2 Based on the fmff C"'.)mponent of the autocorrelation function for polarited light scattering at finite angles, Schmitz and Schurr 4 then presented translational diffusion coefficients D 20 ,w varying from O. 95X 10-8 to 1. 9X 10-8 cm 2 /sec for C-T DNA measured at temperatures ranging from 56 to 86 °C.Recently, Schmitz and Pecora 5 again used a 400-point Saicor 43A autocorrelator and Showed that the timecorrelation function of C-T DNA and A DNA contained contributions from at least two relaxation processes. The authors knew that, in the limit of infinite dilution, most light-scattering theories applicable to macromolecules predicted the longest relaxation time to be due to translational diffusion. 6 Nevertheless, they concluded that, in the case of DNA, the longest time could be interpreted as possible contributions due to restricted translational motion or anisotropic diffusion. Unfortunately, in their study of C-T DNA, they were mainly interested in the behavior of rf2) and failed to report their findings on the high-frequency component (r?»). Secondly, the time-correlation functions were analyzed mainly according to Eq. (1. 2) even though in attempting to fit their measured data to Eq. (1. 2) several computations failed to converge. Again, Schmitz and Pecora 5 observed that Eq. (1. 3) holds, with r1 2 ) being the low-

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Spectral Distribution of the Light Scattered from Rodlike Macromolecules in Solution

Cited by 49 publications

References 6 publications

Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy

Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy

Effects of polydispersity, branching and chain stiffness on quasielastic light scattering

Dyamics of calf-thymus DNA by single-clipped photon correlation

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