Spectral characterization of fluorescent 5-iodoacetamidotetramethylrhodamine and its N-acetylcysteine derivative

Zandvoort, Marc A. M. J. van; Vossen, Dirk L. J.; Ginkel, G. van; Torre, Renato; Bartolini, Paolo; Ricci, Marilena; Thomas‐Oates, Jane; Zuilhof, Han

doi:10.1039/a904341a

Cited by 10 publications

(8 citation statements)

References 62 publications

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“…The absorption and fluorescence dipole moments are situated in the plane of the fluorescent molecule. 22 This implies that the Wigner rotation matrices for ⍀ M A , ⍀ M E , and…”

Section: ͑10͒mentioning

confidence: 99%

“…21 In the case of rhodamine, the angle between A and B is 23°. 22 The direction of the absorption dipole moment depends on the wavelength of excitation. When the probe and bleach beams have the same wavelength, the corresponding absorption dipole moments A and B are identical.…”

Section: A Principle Of Polarized Frapmentioning

confidence: 99%

“…In our experiments the wavelengths of the probe ͑514.5 nm͒ and bleach ͑532 nm͒ beams are somewhat different, but still within the same excitation band for rhodamine. 22 The probability P A that a fluorophore absorbs a photon with polarization Â produced by the probe beam is proportional to…”

Section: A Principle Of Polarized Frapmentioning

confidence: 99%

“…22 The experimental geometry is generally chosen to eliminate the polarization dependence of the fluorescence emission, D 0m 2 (⍀ EB ), or the absorption, D 0m 2 (⍀ AB ). In the theoretical paper of Wegener 17 on pFRAP various possible geometries are specified.…”

Section: ͑14͒mentioning

confidence: 99%

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Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Lettinga

Koenderink

Kuipers

et al. 2004

The Journal of Chemical Physics

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We report a polarized fluorescence recovery after photobleaching ͑pFRAP͒ method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized laser light. The rotational mobilities of the fluorescent particles can be extracted from the relaxation kinetics of the postbleach fluorescence polarization anisotropy. Our pFRAP setup has access to correlation times over a range of time scales from tens of microseconds to tens of seconds, and is highly sensitive, so very low concentrations of labeled particles can be probed. We present a detailed description of the theoretical background of pFRAP. The performance of the equipment is demonstrated for fluorescent colloidal silica spheres, dispersed in pure solvents as well as in fd-virus suspensions.

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“…The absorption and fluorescence dipole moments are situated in the plane of the fluorescent molecule. 22 This implies that the Wigner rotation matrices for ⍀ M A , ⍀ M E , and…”

Section: ͑10͒mentioning

confidence: 99%

Section: A Principle Of Polarized Frapmentioning

confidence: 99%

Section: A Principle Of Polarized Frapmentioning

confidence: 99%

Section: ͑14͒mentioning

confidence: 99%

See 2 more Smart Citations

Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Lettinga

Koenderink

Kuipers

et al. 2004

The Journal of Chemical Physics

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“…This method was used in (polarized) fluorescence after photobleaching [(p)FRAP] 40 for the investigation of the rotational diffusion of particles in solution 41 and the incorporation of dye molecules in membranes 42,43 . It relies on the fact, that dye molecules preferentially absorb and emit light of specific polarization 44 . We introduce two new methods to get mechanical information of colloidal systems on a single particle level via confocal microscopy.…”

Section: Introductionmentioning

confidence: 99%

Experimental analysis of single particle deformations and rotations in colloidal and granular systems

Roth,

Franzmann,

D'Acunzi

et al. 2011

Preprint

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Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the three dimensional positions and translational motions of all constituent (spherical) particles in the observed volume. Though, particle deformations or rotational motions were hardly addressed. Here we present preparation and image processing techniques to also extract the deformation and rotational state of the particles. The deformation analysis is worked out for particles with a hollow sphere-like fluorescence intensity distribution that are more sensitive to small deformations. In the second case of rotations we utilize the angle dependence of the light absorption of the incorporated dye molecules to prepare optically anisotropic particles in analogy to polarized fluorescence after photobleaching. Rotations of these particles are expressed as intensity fluctuations in the confocal images. In contrast to existing methods our techniques do not reduce the quality of the actual particle localization. They can help understanding complex reorganization processes in arrested states of colloidal and granular materials during aging or under external stimuli such as shear or compression.

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A novel probe for determination of electrical surface potential of surfactant micelles: N,N'‐di‐n‐octadecylrhodamine

Mchedlov‐Petrossyan

Vodolazkaya

Yakubovskaya

et al. 2007

J of Physical Organic Chem

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The peculiarities of the structure of the fluorescent dye N,N'-di-n-octadecylrhodamine advantage its using as an interfacial acid-base probe in aqueous micellar solution of colloidal surfactants. Two long hydrocarbon tails of the dye provide similar orientation of both cation and zwitterion on the micelle/water interface, with the ionizing group COOH exposed to the Stern region in all the systems studied. Further, the charge type of the acid-base couple, A þ B AE , ensures similar values of the 'intrinsic' contribution, pK i a , to the 'apparent' pK a a value in micelles of different surfactants. This makes the indicator suitable for determination of electrical surface potentials, C. The pK a a s have been obtained in cationic, anionic, zwitterionic, and nonionic surfactant systems, at various salt background. In total 17 systems were studied. At bulk counterion concentration of ca. 0.05 M, the pK a a values vary from 2.14 AE 0.07 in nÀC 18 H 37 NðCH 3 Þ þ 3 Cl À micelles to 5.48 AE 0.06 in nÀC 16 H 33 OSO À 3 Na þ micelles. The C values, corresponding to the Stern region of micelles, have been evaluated as C¼ 59:16 ðpK i a À pK a a Þ for T ¼ 298.15 K. The pK i a parameter was equated to the average value of 4.23 in nonionic surfactants (4.12-4.32, depending on the surfactant type). For cetyltrimethylammonium bromide and sodium n-dodecylsulfate micelles, the C values (AE(7-11) mV) appeared to be þ118 mVand at bulk Br À concentration 0.019 M and À76 mVat bulk Na þ concentration 0.020 M, respectively. This satisfactorily agrees with the theoretical values þ111 and À84 mV, estimated using the Oshima, Healy, and White equation for these well-defined colloidal systems. Finally, not only absorption, but also fluorescence spectra display the same response to changes in bulk pH.

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Spectral characterization of fluorescent 5-iodoacetamidotetramethylrhodamine and its N-acetylcysteine derivative

Cited by 10 publications

References 62 publications

Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching

Experimental analysis of single particle deformations and rotations in colloidal and granular systems

A novel probe for determination of electrical surface potential of surfactant micelles: N,N'‐di‐n‐octadecylrhodamine

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