Spectral and electrochemical study of host–guest inclusion complex between 2,4-dinitrophenol and β-cyclodextrin

Srinivasan, K.; Stalin, T.; Sivakumar, K.

doi:10.1016/j.saa.2012.03.066

Cited by 71 publications

(17 citation statements)

References 24 publications

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“…It would be interesting to investigate whether Nbp35 and Cfd1 interact to each other and form complex in E. histolytica . Protein docking between Nbp35 and Cfd1 was done using PatchDock server ( http://bioinfo3d.cs.tau.ac.il/PatchDock/ ) [54] with default settings. This server is widely used for the protein-protein docking studies [54] – [56] and its algorithm based on rigid docking with surface flexibility addressed by intermolecular penetration.…”

Section: Methodsmentioning

confidence: 99%

Interaction between Nbp35 and Cfd1 Proteins of Cytosolic Fe-S Cluster Assembly Reveals a Stable Complex Formation in Entamoeba histolytica

et al. 2014

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Iron-Sulfur (Fe-S) proteins are involved in many biological functions such as electron transport, photosynthesis, regulation of gene expression and enzymatic activities. Biosynthesis and transfer of Fe-S clusters depend on Fe-S clusters assembly processes such as ISC, SUF, NIF, and CIA systems. Unlike other eukaryotes which possess ISC and CIA systems, amitochondriate Entamoeba histolytica has retained NIF & CIA systems for Fe-S cluster assembly in the cytosol. In the present study, we have elucidated interaction between two proteins of E. histolytica CIA system, Cytosolic Fe-S cluster deficient 1 (Cfd1) protein and Nucleotide binding protein 35 (Nbp35). In-silico analysis showed that structural regions ranging from amino acid residues (P33-K35, G131-V135 and I147-E151) of Nbp35 and (G5-V6, M34-D39 and G46-A52) of Cfd1 are involved in the formation of protein-protein complex. Furthermore, Molecular dynamic (MD) simulations study suggested that hydrophobic forces surpass over hydrophilic forces between Nbp35 and Cfd1 and Van-der-Waal interaction plays crucial role in the formation of stable complex. Both proteins were separately cloned, expressed as recombinant fusion proteins in E. coli and purified to homogeneity by affinity column chromatography. Physical interaction between Nbp35 and Cfd1 proteins was confirmed in vitro by co-purification of recombinant Nbp35 with thrombin digested Cfd1 and in vivo by pull down assay and immunoprecipitation. The insilico, in vitro as well as in vivo results prove a stable interaction between these two proteins, supporting the possibility of its involvement in Fe-S cluster transfer to target apo-proteins through CIA machinery in E. histolytica. Our study indicates that initial synthesis of a Fe-S precursor in mitochondria is not necessary for the formation of Cfd1-Nbp35 complex. Thus, Cfd1 and Nbp35 with the help of cytosolic NifS and NifU proteins can participate in the maturation of non-mitosomal Fe-S proteins without any apparent assistance of mitosomes.

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Section: Methodsmentioning

confidence: 99%

Interaction between Nbp35 and Cfd1 Proteins of Cytosolic Fe-S Cluster Assembly Reveals a Stable Complex Formation in Entamoeba histolytica

et al. 2014

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“…The substantial decrease in the crystallinity of solid complex in comparison with β-CD and BPY, and the amorphous character exhibited by the inclusion complex proves the nano hydrophobic interaction between β-CD and BPY [74,75].…”

Section: Powder Xrd Analysismentioning

confidence: 99%

Preparation, physicochemical analysis and molecular modeling investigation of 2,2′-Bipyridine: β-Cyclodextrin inclusion complex in solution and solid state

Periasamy

Kothainayaki

Sivakumar

2015

Journal of Molecular Structure

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“…These results provide further evidence for the formation of different complexes between IMC and molecular containers. The surface morphology micrographs of 2,4‐dinitrophenol (2,4‐DNP) in the presence of β‐CD was reported by Srinivason et al and they observed the different surface morphological images of the guest (2,4‐DNP), host (β‐CD) and the guest‐induced host solid complexes, which are completely different 59. The surface morphology image of the formation of supramolecular CD based host–guest complexation has also been reported widely 60.…”

Section: Resultsmentioning

confidence: 91%

A New Remote Control for Traceless meta‐CH Olefination of Phenols

Martı́nez¹,

Muñiz²

2013

ChemCatChem

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The development of a suitable methodology for the introduction of functional groups into the core of aromatic molecules has continued to be an indicator of synthetic progress for over a century. It was already considered of utmost importance nearly 150 years ago, when Kekule dedicated his Chemie der Benzolderivate oder der aromatischen Verbindungen to this particular topic. [1] Within the field of modern arene chemistry, [2] the general concept of direct CÀH functionalization of arenes represents an area with particularly impressive advances over recent years. Classic concepts rely on suitable directing groups for ortho-functionalization. This concept was widely explored for stoichiometric ortho-lithiation, [3] as well as in more recent developments employing transition metal based catalysts, which rely on cyclometalation reactions, where palladium and ruthenium have played prominent roles.[4] In addition to conventional ortho-functionalization, some groups have recently started to explore the development of suitable catalytic conditions for meta-selective CÀH derivatization of arenes. [5] Some time ago, Yu described an important break-through in the field of palladium catalysis. By designing suitable directing groups containing nitrile substituents, his group was able to devise an efficient chelation control in order to generate a coordinated palladium complex A and to direct the cyclopalladation to proceed preferentially at the meta-position (Scheme 1). [6] Although representing a striking methodological accomplishment, removal of the directing group was not straightforward. Readily cleavable directing groups have emerged among recent progress in the area of palladium-catalyzed CÀH derivatization. [7] Following this approach, Yu has now built upon this earlier concept of nitrile-chelation control for palladium-catalyzed position-selective olefination of arenes. This new protocol contains two important accomplishments: the parent arene is a phenol unit and the directing group undergoes traceless removal. Directing a CÀH functionalization into the meta-position of a phenol derivative constitutes a chemical transformation that is not possible through classic aromatic substitution chemistry. To realize such meta-position selectivity, Yu recurred to an a-phenoxy acetamide bearing two nitrile-chelating groups. Upon coordination to palladium, the combination of two appropriate groups should place the metal in close proximity to the meta-position of the phenol substrate, thereby promoting the desired CÀH palladation to take place selectively (Scheme 2).Based on this elegant design, the desired meta-functionalization became indeed possible through a 13-membered cyclopalladated intermediate. Under catalytic conditions, the concept of meta-CÀH functionalization was validated for a series of olefination reactions. A catalyst from palladium acetate, N-acetyl glycine as ligand 2 and silver acetate as terminal oxidant generate the best reaction conditions for the conversion of a number of phenol derivatives 1 (Scheme 3). ...

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Spectral and electrochemical study of host–guest inclusion complex between 2,4-dinitrophenol and β-cyclodextrin

Cited by 71 publications

References 24 publications

Interaction between Nbp35 and Cfd1 Proteins of Cytosolic Fe-S Cluster Assembly Reveals a Stable Complex Formation in Entamoeba histolytica

Interaction between Nbp35 and Cfd1 Proteins of Cytosolic Fe-S Cluster Assembly Reveals a Stable Complex Formation in Entamoeba histolytica

Preparation, physicochemical analysis and molecular modeling investigation of 2,2′-Bipyridine: β-Cyclodextrin inclusion complex in solution and solid state

A New Remote Control for Traceless meta‐CH Olefination of Phenols

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