Spectra and physical properties of liposomes and monolayers of polymerizable phospholipids containing diacetylene groups in one or both acyl chains

Johnston, David; McLean, Larry R.; Whittam, Mary A.; Clark, Anthony D.; Chapman, D.

doi:10.1021/bi00282a024

Cited by 73 publications

(37 citation statements)

References 26 publications

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“…Lipid mixtures prepared from DNPC/DC 8,9 PC (formulation #2c, 1:1 mol ratio), exhibited rapid photo-crosslinking upon exposure to UV radiation as indicated by spectral shifts in the UV-VIS spectrum [14] (data not shown) [17,18]. However, none of the DNPC (matrix lipid 2) formulations entrapped any calcein.…”

Section: Resultsmentioning

confidence: 99%

Design of liposomes containing photopolymerizable phospholipids for triggered release of contents

Yavlovich

Singh

Tarasov

et al. 2009

J Therm Anal Calorim

135

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We describe a novel class of light-triggerable liposomes prepared from a photo-polymerizable phospholipid DC8,9PC (1,2- bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine) and DPPC (1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine). Exposure to UV (254 nm) radiation for 0–45 minutes at 25°C resulted in photo-polymerization of DC8,9PC in these liposomes and the release of an encapsulated fluorescent dye (calcein). Kinetics and extents of calcein release correlated with mol% of DC8,9PC in the liposomes. Photopolymerization and calcein release occurred only from DPPC/DC8,9PC but not from Egg PC/DC8,9PC liposomes. Our data indicate that phase separation and packing of polymerizable lipids in the liposome bilayer are major determinants of photo-activation and triggered contents release.

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Section: Resultsmentioning

confidence: 99%

Design of liposomes containing photopolymerizable phospholipids for triggered release of contents

Yavlovich

Singh

Tarasov

et al. 2009

J Therm Anal Calorim

135

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“…Upon polymerization, the bilayers became significantly less soluble in organic solvents or detergent solutions. [17][18][19][20] In addition, decrease in the lateral diffusion constant and the permeation coefficient of the bilayers was observed. 21,22 Micropatterning of SPBs by lithographic photopolymerization of lipid bilayers aims to achieve both spatial control and stabilization of biomimetic membranes.…”

Section: Introductionmentioning

confidence: 95%

Photopolymerization of Diacetylene Lipid Bilayers and Its Application to the Construction of Micropatterned Biomimetic Membranes

et al. 2002

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Photopolymerization of diacetylene-containing amphiphiles in substrate-supported bilayers has been studied in connection with the development of a new fabrication strategy of micropatterned biomimetic membrane systems. Two types of amphiphilic diacetylene molecules were compared, one being a monoalkyl phosphate, phosphoric acid monohexacosa-10,12-diynyl ester (1), and the other being a phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (2). The bilayers of monomeric diacetylene amphiphiles were deposited onto substrates by the Langmuir-Blodgett/Langmuir-Schaefer methods. Both amphiphiles could be polymerized successfully on oxide and polymer-coated substrates. However, the phospholipid (2) showed a markedly higher mechanical stability of the polymerized bilayer films compared with the monoalkyl phosphate (1). Micropatterns were imposed in the bilayers by using a physical mask to protect the monomeric lipids partially upon UV irradiation. In the case of 2, monomeric bilayers could be removed selectively by ethanol after the lithographic photopolymerization, resulting in the formation of wells between the polymerized bilayers. These wells were filled with fluid phosphatidylcholine bilayers by fusion of vesicles. The rigid polymeric bilayer and the fluid biomimetic bilayer could be integrated as a composite bilayer membrane with defined spatial patterns, offering new possibilities to construct complex and versatile biomimetic membrane systems.

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“…When polymeric units are completely formed, after being subjected to 20 polymerization cycles [9], the polymer backbone shows strong absorbance in the visible region of the spectrum [11] and the degree of polymerization of the membrane is related to both the magnitude and the wavelength of visible absorbance peaks [7,10]. A scheme of the polymer formed is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Absorbance around 480 nm corresponds to shorter polymers and absorbance around 520 nm to longer conjugation of cross-bridges formed between linear polymers through intra-and inter-molecular C-chains [10,19]. According to Ahl et al [19], the amount of polymeric units can be calculated from the maxima detected in the Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…The polymerization process was evaluated by measuring the absorbance in the UV-visible (UV-vis) region after 20 polymerization cycles [7,[9][10][11]. The UV-vis absorption profile of the different samples was analyzed using a Nanodrop ND-100 spectrophotometer (Thermo Scientific) with a 1-mM final lipid concentration in the range of 400-700 nm.…”

Section: Uv-vis Spectrophotometrymentioning

confidence: 99%

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Intratracheal Instillation of Lipopolymeric Vectors and the Effect on Mice Lung Physiology

Xisto

Temprana

Martini

et al. 2012

Cell Physiol Biochem

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Background/Aims: The current study compared the effects of intratracheal administration of different lipopolymeric vectors on lung function and histology in normal mice. Methods: Forty-eight BALB/c mice were randomly divided into 8 groups (6/group). All animals received intratracheal instillation of the following suspensions: polymerized [(A) 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC):1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero3-phosphocholine (DC8,9PC):1,2-dioleoyl3-trimethylammonium-propane (DOTAP), (B) DMPC:DC8,9PC:stearylamine (SA), (C) DMPC:DC8,9PC:myristoylcholine chloride (MCl)]; nonpolymerized [(D) DMPC:DC8,9PC:DOTAP, (E) DMPC:DC8,9PC:SA, (F) DMPC:DC8,9PC:MCl] together with plasmid DNA; vehicle (control), and pDsRed2-N1 plasmid DNA (DNA). At 24 h, the survival rate, lung mechanics (resistive and viscoelastic pressure, static elastance) and morphometry were analyzed. Results: The survival rate was 50% in D, 40% in E and F, and 100% in the CTRL, DNA, A, B and C groups. Animals from groups D, E, and F that died presented diffuse pulmonary hemorrhagic capillaritis. Lung mechanics, the fraction of normal and collapsed alveoli, as well as the number of polymorphonuclear and mononuclear cells in lung tissue were similar in all surviving mice. Conclusion: Intratracheal instillation of polymerized particles is safe compared with nonpolymerized formulations and may be used for future gene/drug therapy.

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Spectra and physical properties of liposomes and monolayers of polymerizable phospholipids containing diacetylene groups in one or both acyl chains

Cited by 73 publications

References 26 publications

Design of liposomes containing photopolymerizable phospholipids for triggered release of contents

Design of liposomes containing photopolymerizable phospholipids for triggered release of contents

Photopolymerization of Diacetylene Lipid Bilayers and Its Application to the Construction of Micropatterned Biomimetic Membranes

Intratracheal Instillation of Lipopolymeric Vectors and the Effect on Mice Lung Physiology

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