Specimen acquisition: ROSEs, gardeners, and gatekeepers

Kraft, Adele

doi:10.1002/cncy.21870

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…The quality of cytological specimens may be improved by the use of ultrasound guided‐FNA and by rapid on‐site evaluation (ROSE) . In the laboratory, lymphoid lesions commonly pose interpretive challenges.…”

Section: Introductionmentioning

confidence: 99%

Investigation of lymphoid lesions of the head and neck using combined fine needle aspiration cytology and flow cytometry: Accuracy and pitfalls

et al. 2019

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Objective We reviewed the diagnostic utility of combined fine needle aspiration cytology (FNAC) and flow cytometry (FC) in the diagnosis of lymphoid lesions of the head and neck. Method In total, 1402 patients with combined FNAC‐FC reports were correlated with follow‐up information. Rapid on‐site evaluation (ROSE) of cytological specimens was performed in 52% of cases. Results In total, 211 lymphoid malignancies were identified, including 198 non‐Hodgkin lymphoma (NHL) and 13 Hodgkin lymphoma (HL). Accuracy measures for NHL were: sensitivity 95.5%; specificity 99.9%; PPV 99.5%; NPV 99.2%; accuracy 99.3%. Only seven of 13 cases of HL were detected by FNAC‐FC. False negative cases included HL (six cases), diffuse large B‐cell lymphoma (four), T‐cell lymphoma (two), follicular lymphoma (one), marginal zone cell lymphoma (one) and B‐cell NHL, not otherwise specified (one). Two false positive results were identified: one immunoblastic hyperplasia reported as suspicious for HL and one case reported as suggestive of NHL that was found to be reactive hyperplasia. Cases collected with ROSE had a significantly lower rate (P < 0.0001) of insufficient cells for FC analysis (7.0%) than cases where ROSE was not performed (16.4%). Sensitivity (P < 0.0001) and NPV (P = 0.0023) were significantly higher for ROSE‐collected specimens. None of the false‐negative NHL cases had ROSE performed. Conclusions FNAC‐FC is a highly sensitive and specific test for NHL. Diagnostic errors mostly involved HL, large cell lymphomas and T‐cell lymphomas. ROSE results in a significantly higher adequacy rate for FC and higher sensitivity for NHL.

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Section: Introductionmentioning

confidence: 99%

Investigation of lymphoid lesions of the head and neck using combined fine needle aspiration cytology and flow cytometry: Accuracy and pitfalls

et al. 2019

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“…1–5 Furthermore, ROSE allows proper triage of the material collected, directing the operator to obtain material for cultures if an infectious etiology and for flow cytometry studies if a lymphoproliferative disorder is a diagnostic consideration as well improving the adequacy rate for molecular studies. 6, 7…”

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confidence: 99%

Telecytology for rapid on-site evaluation: current status

Lin

2018

Journal of the American Society of Cytopathology

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“…14 In addition, it helps provide high-quality tissue for clinical workup, including ancillary studies, and for potential research studies. 11,15 In large institutions, however, there are challenges for implementing ROSE. The main challenge is that multiple procedures occur simultaneously, which may limit a pathologist's availability for ROSE procedures.…”

Section: Introductionmentioning

confidence: 99%

Incorporating cytologic adequacy assessment into precision oncology workflow using telepathology: An institutional experience

Zoughbi

Kim

Alperstein

et al. 2021

Cancer Cytopathology

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BACKGROUND:Tumor sample quality and quantity determine the success of somatic mutation analysis. Thus, a rapid on-site evaluation (ROSE) tumor cytology adequacy assessment was incorporated into the workflow of precision oncology at Weill Cornell Medicine in New York City. Optimal samples were obtained from 68 patients with metastatic cancer. METHODS:Cytopathologists performed ROSE on fine-needle aspirate samples via telepathology, and subsequently core-needle biopsies were obtained. In a retrospective manner, the concordance between adequacy assessment and the success rate of the procedure was evaluated to obtain sufficient tumor tissue for next-generation sequencing (NGS). RESULTS:Out of the 68 procedures, 43 were documented as adequate and 25 were documented as inadequate. The diagnostic yield of adequate procedures was 100%. Adequacy evaluation predicted the success rate of molecular profiling in 40 of 43 procedures (93%; 95% CI, 80.9-98.5 procedures). The success rate of molecular testing was significantly higher in the adequate group: 93% compared with 32% in the inadequate group (P < .0005). Seven procedures that failed to provide quality material for mutational analysis and pathological diagnosis were evaluated as inadequate. Cell block provided sufficient DNA for NGS in 6 cases. In 2 cases, a core biopsy could not be performed; hence, the fineneedle aspirate material confirmed the diagnosis and was used for NGS testing. CONCLUSION: These results support the incorporation of ROSE into the workflow of precision oncology to obtain high-quality tissue samples from metastatic lesions. In addition, NGS testing of concurrent cytology specimens with adequate cellularity can be a surrogate for NGS testing of biopsy specimens.

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Specimen acquisition: ROSEs, gardeners, and gatekeepers

Cited by 12 publications

References 36 publications

Investigation of lymphoid lesions of the head and neck using combined fine needle aspiration cytology and flow cytometry: Accuracy and pitfalls

Investigation of lymphoid lesions of the head and neck using combined fine needle aspiration cytology and flow cytometry: Accuracy and pitfalls

Telecytology for rapid on-site evaluation: current status

Incorporating cytologic adequacy assessment into precision oncology workflow using telepathology: An institutional experience

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