Specificity of yeast (Saccharomyces cerevisiae) in removing carbohydrates by fermentation

Yoon, Seung-Heon; Mukerjea, Rupendra; Robyt, John F.

doi:10.1016/s0008-6215(03)00097-1

Cited by 117 publications

(92 citation statements)

References 7 publications

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“…In their study, immobilized cells of the bacterium Zymomonas mobilis were used to remove glucose, fructose, and sucrose from food-grade oligosaccharide mixtures, which were completely fermented within 12 h. The fermentation end products were ethanol and carbon dioxide, a minimal amount of sorbitol also being formed. Similarly to Z. mobilis, Saccharomyces cerevisiae also showed the ability to ferment some common mono-and disaccharides (fructose, glucose, galactose, and sucrose) from a mixture of sugars, while oligosaccharides with four or more monosaccharide units were not fermented (Yoon et al 2003;Goulas et al 2007;Hernández et al 2009). The microbial treatment seems to be a good alternative for increasing the percentage of FOS in a mixture through the removal of mono-and disaccharides, this process being adequate to be used during the enzymatic synthesis of FOS.…”

Section: Fos Production Byssfmentioning

confidence: 99%

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Dominguez

Rodrigues

Lima

et al. 2013

Food Bioprocess Technol

137

108

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Over the past years, many researchers have suggested that deficiencies in the diet can lead to disease states and that some diseases can be avoided through an adequate intake of relevant dietary components. Recently, a great interest in dietary modulation of the human gut has been registered. Prebiotics, such as fructooligosaccharides (FOS), play a key role in the improvement of gut microbiota balance and in individual health. FOS are generally used as components of functional foods, are generally regarded as safe (generally recognized as safe status-from the Food and Drug Administration, USA), and worth about 150€ per kilogram. Due to their nutrition-and health-relevant properties, such as moderate sweetness, low carcinogenicity, low calorimetric value, and low glycemic index, FOS have been increasingly used by the food industry. Conventionally, FOS are produced through a two-stage process that requires an enzyme production and purification step in order to proceed with the chemical reaction itself. Several studies have been conducted on the production of FOS, aiming its optimization toward the development of more efficient production processes and their potential as food ingredients. The improvement of FOS yield and productivity can be achieved by the use of different fermentative methods and different microbial sources of FOSproducing enzymes and the optimization of nutritional and culture parameter; therefore, this review focuses on the latest progresses in FOS research such as its production, functional properties, and market data.

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Section: Fos Production Byssfmentioning

confidence: 99%

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Dominguez

Rodrigues

Lima

et al. 2013

Food Bioprocess Technol

137

108

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“…cerevisiae cannot grow on L-rhamnose as the sole carbon source (Barnett et al 1990). Moreover, Yoon et al (2003) showed the inability of immobilized S. cerevisiae to remove rhamnose from a carbohydrate stream under anaerobic conditions. Indeed, the S. cerevisiae genome does not reveal genes with a clear homology to genes encoding rhamnose-metabolizing enzymes (data not shown).…”

Section: Rhamnosementioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

433

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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“…After this gel was dissolved in An alternative purification method was also investigated that might be more suitable for largescale operations. In literature, baker's yeast Saccharomyces cerevisiae is often used to metabolize sugar by-products in bioconversion reactions [30][31][32] while phosphate can be removed by chemical precipitation [16]. However, Yoon et al [32] have noted that trehalose is only partially hydrolyzed by baker's yeast.…”

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

“…In literature, baker's yeast Saccharomyces cerevisiae is often used to metabolize sugar by-products in bioconversion reactions [30][31][32] while phosphate can be removed by chemical precipitation [16]. However, Yoon et al [32] have noted that trehalose is only partially hydrolyzed by baker's yeast. This was confirmed in our experiments, which implied the need for the addition of a trehalase to assist in carbohydrate removal.…”

Section: Purification Of β β β βGlc1pmentioning

confidence: 99%

Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

Borght¹,

Desmet²,

Soetaert³

2010

Biotechnology Journal

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beta-D-Glucose-1-phosphate (beta Glc1P) is an efficient glucosyl donor for both enzymatic and chemical glycosylation reactions but is currently very costly and not available in large amounts. This article provides an efficient production method of beta Glc1P from trehalose and phosphate using the thermostable trehalose phosphorylase from Thermoanaerobacter brockii. At the process temperature of 60 C, Escherichia coli expression host cells are lysed and cell treatment prior to the reaction is, therefore, not required. In this way, the theoretical maximum yield of 26% could be easily achieved. Two different purification strategies have been compared, anion exchange chromatography or carbohydrate removal by treatment with trehalase and yeast, followed by chemical phosphate precipitation. In a next step, beta Glc1P was precipitated with ethanol but this did not induce crystallization, in contrast to what ist observed with other glycosylphosphates. After conversion of the product to its cyclohexylammonium salt, however, crystals could be readily obtained. Although both purification methods were quantitative (>99% recovery), a large amount of product (50%) was lost during crystallization. Nevertheless, a production process for crystalline beta Glc1P is now available from the cheap substrates trehalose and inorganic phosphate

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Specificity of yeast (Saccharomyces cerevisiae) in removing carbohydrates by fermentation

Cited by 117 publications

References 7 publications

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

An Overview of the Recent Developments on Fructooligosaccharide Production and Applications

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Enzymatic production of β‐D‐glucose‐1‐phosphate from trehalose

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