Specificity of the peroxisome proliferation response in mussels exposed to environmental pollutants

Cajaraville, Miren P.; Ortiz-Zarragoitia, Maren

doi:10.1016/j.aquatox.2006.02.016

Cited by 33 publications

(10 citation statements)

References 37 publications

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“…Peroxisome proliferation in bivalve species is actually used as a biomarker for monitoring the health of aquatic environments [116]. Organic xenobiotics such as polycyclic aromatic hydrocarbons (PAHs), phthalates and bisphenol-A, increase the number and volume of peroxisomes and induce peroxisomal β-oxidation enzyme acyl coenzyme A (acyl-CoA) oxidase in Mytilus edulis and M. galloprovincialis [117–119]. Vertebrate peroxisome proliferation and acyl-CoA are regulated by PPARs [120, 121] and disturbance of PPAR regulated genes has been observed after exposure to the aforementioned xenobiotics [122–124].…”

Section: Discussionmentioning

confidence: 99%

The nuclear receptor gene family in the Pacific oyster, Crassostrea gigas, contains a novel subfamily group

et al. 2014

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BackgroundNuclear receptors are a superfamily of transcription factors important in key biological, developmental and reproductive processes. Several of these receptors are ligand- activated and through their ability to bind endogenous and exogenous ligands, are potentially vulnerable to xenobiotics. Molluscs are key ecological species in defining aquatic and terrestrial habitats and are sensitive to xenobiotic compounds in the environment. However, the understanding of nuclear receptor presence, function and xenobiotic disruption in the phylum Mollusca is limited.ResultsHere, forty-three nuclear receptor sequences were mined from the genome of the Pacific oyster, Crassostrea gigas. They include members of NR0-NR5 subfamilies, notably lacking any NR6 members. Phylogenetic analyses of the oyster nuclear receptors have been conducted showing the presence of a large novel subfamily group not previously reported, which is named NR1P. Homologues to all previous identified nuclear receptors in other mollusc species have also been determined including the putative heterodimer partner retinoid X receptor, estrogen receptor and estrogen related receptor.ConclusionC. gigas contains a highly diverse set of nuclear receptors including a novel NR1 group, which provides important information on presence and evolution of this transcription factor superfamily in invertebrates. The Pacific oyster possesses two members of NR3, the sex steroid hormone receptor analogues, of which there are 9 in humans. This provides increasing evidence that steroid ligand specific expansion of this family is deuterostome specific. This new knowledge on divergence and emergence of nuclear receptors in C. gigas provides essential information for studying regulation of molluscan gene expression and the potential effects of xenobiotics.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-369) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The nuclear receptor gene family in the Pacific oyster, Crassostrea gigas, contains a novel subfamily group

et al. 2014

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“…In laboratory studies, mussels also showed increased AOX1 activities after exposure to different environmentally relevant pollutants (Cajaraville et al, 2003;Cajaraville and Ortiz-Zarragoitia, 2006). Similarly, induction of AOX1 activity has been reported in different fish species inhabiting environments with high burdens of organic toxic compounds (Bilbao et al, 2006a) and in different laboratory experiments after treatment with different chemical compounds (Ortiz-Zarragoitia and Cajaraville, 2005;Scarano et al, 1994;Yang et al, 1990).…”

Section: Introductionmentioning

confidence: 86%

“…Due to the environmental relevance of the inducible pathway as a biomarker of exposure to xenobiotics that might act as peroxisome proliferators, the present study is focused on AOX1, MFP1 and THIO. AOX1 is the first and rate-limiting enzyme of the inducible peroxisomal β-oxidation pathway and increased AOX1 activity is usually measured as peroxisome proliferation marker (Cajaraville et al, 2003;Cajaraville and Ortiz-Zarragoitia, 2006;. MFP1 catalyses the second and third steps of the pathway together with an isomerisation step, that is necessary to catabolise unsaturated substrates (Hiltunen and Qin, 2000;Mannaerts et al, 2000;Reddy and Hashimoto, 2001).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression pattern of peroxisomal β-oxidation genes palmitoyl-CoA oxidase, multifunctional protein and 3-ketoacyl-CoA thiolase in mussel Mytilus galloprovincialis and thicklip grey mullet Chelon labrosus

Bilbao

Cajaraville

Cancio

2009

Gene

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“…Exposure to 500 mg DEHP l 21 for 21 days was shown to result in a significant increase in the catalase and acyl-CoA oxidase (AOX) activity and an inhibition of the superoxide and manganate superoxide dismutase in M. galloprovincialis. The peroxisomal volume density in the digestive gland of M. edulis was significantly enhanced at 50 mg diallylphthalate (DAP) per litre after three weeks exposure, whereas the chemical had no significant effect on AOX activity in this species (Cajaraville & Ortiz-Zarragoitia 2006). DAP was able to decrease the phospho-protein level (a mussel VTG-like protein) but did not impair ovarian follicles, oocytes and spermatogenesis in M. edulis when exposed to 50 mg DAP l 21 for three weeks (Aarab et al 2006).…”

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confidence: 99%

“…Metallothionein MT20 gene expression was significantly downregulated; catalase, GSH transferase and GSSG reductase exhibited a changed activity pattern and total glutathione content was enhanced at all test concentrations. Unlike for the phthalate DAP, a study by Cajaraville & Ortiz-Zarragoitia (2006) found no effects of BPA on AOX activity or peroxisomal volume density.…”

mentioning

confidence: 99%

A critical analysis of the biological impacts of plasticizers on wildlife

Oehlmann

Schulte‐Oehlmann

Kloas

et al. 2009

Phil. Trans. R. Soc. B

746

341

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This review provides a critical analysis of the biological effects of the most widely used plasticizers, including dibutyl phthalate, diethylhexyl phthalate, dimethyl phthalate, butyl benzyl phthalate and bisphenol A (BPA), on wildlife, with a focus on annelids (both aquatic and terrestrial), molluscs, crustaceans, insects, fish and amphibians. Moreover, the paper provides novel data on the biological effects of some of these plasticizers in invertebrates, fish and amphibians. Phthalates and BPA have been shown to affect reproduction in all studied animal groups, to impair development in crustaceans and amphibians and to induce genetic aberrations. Molluscs, crustaceans and amphibians appear to be especially sensitive to these compounds, and biological effects are observed at environmentally relevant exposures in the low ng l 21 to mg l 21 range. In contrast, most effects in fish (except for disturbance in spermatogenesis) occur at higher concentrations. Most plasticizers appear to act by interfering with the functioning of various hormone systems, but some phthalates have wider pathways of disruption. Effect concentrations of plasticizers in laboratory experiments coincide with measured environmental concentrations, and thus there is a very real potential for effects of these chemicals on some wildlife populations. The most striking gaps in our current knowledge on the impacts of plasticizers on wildlife are the lack of data for long-term exposures to environmentally relevant concentrations and their ecotoxicity when part of complex mixtures. Furthermore, the hazard of plasticizers has been investigated in annelids, molluscs and arthropods only, and given the sensitivity of some invertebrates, effects assessments are warranted in other invertebrate phyla.

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Specificity of the peroxisome proliferation response in mussels exposed to environmental pollutants

Cited by 33 publications

References 37 publications

The nuclear receptor gene family in the Pacific oyster, Crassostrea gigas, contains a novel subfamily group

The nuclear receptor gene family in the Pacific oyster, Crassostrea gigas, contains a novel subfamily group

Cloning and expression pattern of peroxisomal β-oxidation genes palmitoyl-CoA oxidase, multifunctional protein and 3-ketoacyl-CoA thiolase in mussel Mytilus galloprovincialis and thicklip grey mullet Chelon labrosus

A critical analysis of the biological impacts of plasticizers on wildlife

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