Specificity of the effect of dietary cholesterol on rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity (Short Communication)

Abstract

Dietary cholesterol lowers the activity of rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase without affecting various other liver microsomal enzymes. This is consistent with a specific regulatory mechanism and distinguishes the action of cholesterol on 3-hydroxy-3-methylglutaryl-CoA reductase from that of at least one other stimulus known to affect this enzyme.

A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme

“…Dependence of 3-hydroxy-3-methylglutaryl-CoA reductase activity on initial NADPH concentration Liver microsomal fraction was prepared and 3hydroxy-3-methylglutaryl-CoA reductase activity was assayed as described in the text, except that the initial NADPH concentration was varied as shown. Gregory & Booth, 1975) and so does starvation (Regen et al, 1966;Linn, 1967;Hamprecht et al, 1969). The diurnal variation in activity is described in the introduction.…”