Specificity of P2 primary alkylsulphohydrolase induction in the detergent-degrading bacterium <i>Pseudomonas</i> C12B. Effects of alkanesulphonates, alkyl sulphates and other related compounds

Cloves, J M; Dodgson, K. S.; White, Graham F.; Fitzgerald, John W.

doi:10.1042/bj1850013

Cited by 23 publications

(5 citation statements)

References 22 publications

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“…The genus Pseudomonas is well known for its astonishing catabolic versatility, enabling pseudomonads to degrade, transform or accumulate a huge range of industrial compounds. Alkylsulfatase activity has been reported for many Pseudomonas strains such as Pseudomonas C12B (Cloves et al. 1977; Bateman et al.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the sdsA alkyl sulfatase of Pseudomonas sp. ATCC19151 and its involvement in degradation of anionic surfactants

Jovčić

Venturi

Davison

et al. 2010

Journal of Applied Microbiology

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Aims: The presented study was aimed to reveal transcriptional regulation of genes involved in SDS degradation (sdsA and sdsB) in Pseudomonas sp. ATCC19151. In addition, the ability of Pseudomonas sp. ATCC19151 to degrade anionic surfactants present in commercial detergent and septic tank drain was analysed. Methods and Results: Strain ATCC19151, at 30°C, degrades all SDS present in the liquid medium (up to 4% w/v of SDS) within 48 h. ATCC19151 grows in the presence up to 15% (v/v) ‘Fairy’ commercial detergent and mineralizes 35% of present anionic surfactants. Analysis of the sdsA (PsdsA) and divergent sdsB (PsdsB) gene promoter activities revealed that SdsB acts as a positive regulator of sdsA and sdsB transcription. PsdsA and PsdsB activities rose significantly in the presence of the SDS, indicating inducibility of sdsA and sdsB transcription. DNA‐binding assay indicated that SdsB directly regulates the transcription of sdsA and sdsB genes. Strain ATCC19151 grew in a sterile septic tank drain and on commercial detergent as sole source of carbon. Conclusions: SdsA enables Pseudomonas sp. ATCC19151 to utilize SDS as a sole carbon source. SdsB is positive transcriptional regulator of sdsA and sdsB genes. Significance and Impact of the Study: Ability of ATCC19151 to degrade anionic surfactants makes Pseudomonas sp. ATCC19151 a good candidate for bioremediation.

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Section: Introductionmentioning

confidence: 99%

Regulation of the sdsA alkyl sulfatase of Pseudomonas sp. ATCC19151 and its involvement in degradation of anionic surfactants

Jovčić

Venturi

Davison

et al. 2010

Journal of Applied Microbiology

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“…The P1 and P2 enzymes have been purified and characterized in detail. While they have the same exclusive specificity for LPAS, they are distinguishable on the basis of the mechanism of COOOS ester bond cleavage (OOS and COO for P1 and P2, respectively) and the regulation of synthesis (P1 is constitutive and P2 is inducible) (2,6,7). Alkylsulfatase activity has also been found in Pseudomonas aeruginosa (11) and Aerobacter aerogenes (24).…”

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confidence: 99%

Novel Alkylsulfatases Required for Biodegradation of the Branched Primary Alkyl Sulfate Surfactant 2-Butyloctyl Sulfate

Ellis

Hales

Ur-Rehman

et al. 2002

Appl Environ Microbiol

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Recent reports show that contrary to common perception, branched alkyl sulfate surfactants are readily biodegradable in standard biodegradability tests. We report here the isolation of bacteria capable of biodegrading 2-butyloctyl sulfate and the identification of novel enzymes that initiate the process. Enrichment culturing from activated sewage sludge yielded several strains capable of growth on 2-butyloctyl sulfate. Of these, two were selected for further study and identified as members of the genus Pseudomonas. Strain AE-A was able to utilize either sodium dodecyl sulfate (SDS) or 2-butyloctyl sulfate as a carbon and energy source for growth, but strain AE-D utilized only the latter. Depending on growth conditions, strain AE-A produced up to three alkylsulfatases, as shown by polyacrylamide gel electrophoresis zymography. Growth on either SDS or 2-butyloctyl sulfate or in nutrient broth produced an apparently constitutive, nonspecific primary alkylsulfatase, AP1, weakly active on SDS and on 2-butyloctyl sulfate. Growth on 2-butyloctyl sulfate produced a second enzyme, AP2, active on 2-butyloctyl sulfate but not on SDS, and growth on SDS produced a third enzyme, AP3, active on SDS but not on 2-butyloctyl sulfate. In contrast, strain AE-D, when grown on 2-butyloctyl sulfate (no growth on SDS), produced a single enzyme, DP1, active on 2-butyloctyl sulfate but not on SDS. DP1 was not produced in broth cultures. DP1 was induced when residual 2-butyloctyl sulfate was present in the growth medium, but the enzyme disappeared when the substrate was exhausted. Gas chromatographic analysis of products of incubating 2-butyloctyl sulfate with DP1 in gels revealed the formation of 2-butyloctanol, showing the enzyme to be a true sulfatase. In contrast, Pseudomonas sp. strain C12B, well known for its ability to degrade linear SDS, was unable to grow on 2-butyloctyl sulfate, and its alkylsulfatases responsible for initiating the degradation of SDS by releasing the parent alcohol exhibited no hydrolytic activity on 2-butyloctyl sulfate. DP1 and the analogous AP2 are thus new alkylsulfatase enzymes with novel specificity toward 2-butyloctyl sulfate.Synthetic surfactants are components of a variety of household and industrial detergent formulations. Other industrial applications include paints, textiles and fabrics, oil-spill dispersants, concrete, paper, lubricants, and many others (14,15,26). In 1990, world production of synthetic surfactants was 7 megatonnes (Mt) per annum, of which 5.3 Mt came from the United States, the European Union, and Japan (12). Of this part, 1.74 Mt per annum was anionic surfactant, a significant quantity which reflects the high demand for this type of surfactant. The anionic surfactant group embraces a range of compounds distinguished on the basis of chemical structure and includes primary alkyl sulfates, such as sodium dodecyl sulfate (SDS), and linear secondary alkyl sulfates, such as dodecyl 2-sulfate. Synthetic primary alkyl sulfates are based on feedstocks derived from long-chain olefins by us...

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“…Of the fi ve alkyl sulfatases produced by Pseudomonas C12B, two (designated P1 and P2) are active towards primary alkyl sulfates, whereas the other three (SI, S2 and S3) act on secondary alkyl sulfates (Cloves et al 1980 ). Pseudomonas sp.…”

Section: Biological Methodsmentioning

confidence: 99%

Pollutant Diseases, Remediation and Recycling

2013

Environmental Chemistry for a Sustainable World

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Specificity of P2 primary alkylsulphohydrolase induction in the detergent-degrading bacterium Pseudomonas C12B. Effects of alkanesulphonates, alkyl sulphates and other related compounds

Cited by 23 publications

References 22 publications

Regulation of the sdsA alkyl sulfatase of Pseudomonas sp. ATCC19151 and its involvement in degradation of anionic surfactants

Regulation of the sdsA alkyl sulfatase of Pseudomonas sp. ATCC19151 and its involvement in degradation of anionic surfactants

Novel Alkylsulfatases Required for Biodegradation of the Branched Primary Alkyl Sulfate Surfactant 2-Butyloctyl Sulfate

Pollutant Diseases, Remediation and Recycling

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