Specificity of meal pattern analysis as an animal model of determining temporomandibular joint inflammation/pain

Kerins, Carolyn A.; Carlson, David S.; Hinton, Robert J.; Hutchins, Bob; Grogan, David M.; Marr, K.; Kramer, Phillip R.; Spears, Robert; Bellinger, Larry L.

doi:10.1016/j.ijom.2004.08.010

Cited by 28 publications

(50 citation statements)

References 29 publications

(52 reference statements)

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“…The hydraulic cements all decreased inflammation 30 days after pulp capping a mechanical lesion. This decrease in inflammation was associated with a decrease in orofacial pain, which is consistent with previous studies (11,13–16). Future studies could determine whether a reduction in postoperative pain, an indicator of reduced pulpal damage, can be correlated to a reduction in the 30% failure rate resulting from pulp capping of mechanical lesions (32).…”

Section: Discussionsupporting

confidence: 92%

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Capping a Pulpotomy with Calcium Aluminosilicate Cement: Comparison to Mineral Trioxide Aggregates

Kramer

Woodmansey²,

White³

et al. 2014

Journal of Endodontics

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Introduction Calcium aluminate cements have shown little affinity for bacterial growth, low toxicity, and immunogenicity when used as a restoration material, but calcium aluminate cements have not been tested in vivo in pulpotomy procedures. Methods To address this question, a calcium aluminate cement (Quick-Set) was tested along with 2 mineral trioxide aggregates, ProRoot MTA and MTA Plus. These cements were used as a capping agent after pulpotomy. Control rats had no pulpotomy, or the pulpotomy was not capped. Proinflammatory cytokines interleukin (IL)-1β and IL-1α were measured, and histology was performed at 30 and 60 days after capping. The nociceptive response was determined by measuring the lengthening of the rat's meal duration. Results and Conclusions: IL-1β and IL-1α concentrations were reduced in the capped teeth, but no differences were observed among the 3 cements. Dentinal bridging could be detected at both 30 and 60 days with each of the 3 cements, and the pulps were still vital 60 days after capping. Meal duration significantly shortened after placement of the 3 different cements, indicating a nociceptive response, but there were no differences among the materials. Calcium aluminate cements had similar properties to mineral trioxide aggregates and is a viable option for pulpotomy procedures.

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Section: Discussionsupporting

confidence: 92%

“…The nociceptive response was measured by using a behavioral assay, specifically the rat's meal duration. Previous studies have shown that a lengthening of the rat's meal duration correlates to orofacial pain in rats (11-16), and that meal duration has been shown to be significantly longer after pulpotomy (12). …”

mentioning

confidence: 99%

Capping a Pulpotomy with Calcium Aluminosilicate Cement: Comparison to Mineral Trioxide Aggregates

Kramer

Woodmansey²,

White³

et al. 2014

Journal of Endodontics

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“…The movement away from reflex-based measures of pain into operant tasks is becoming more common in the field. Other groups have used non-reflex-based measures like examining meal duration 21,22,23 and thermal heat pain escape paradigms 24 (For a review of other pain measures we suggest our first reference 1 ). The OPAD is able to combine elements of these into a unified measure, the Lick/Face ratio, which examines food intake and the need to escape from painful stimuli.…”

Section: Discussionmentioning

confidence: 99%

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Anderson

Mills

Nolan

et al. 2013

JoVE

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We present an operant system for the detection of pain in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) assesses pain behaviors in a more clinically relevant way by not relying on reflex-based measures of nociception. Food fasted, hairless (or shaved) rodents are placed into a Plexiglas chamber which has two Peltier-based thermodes that can be programmed to any temperature between 7°C and 60 °C. The rodent is trained to make contact with these in order to access a reward bottle. During a session, a number of behavioral pain outcomes are automatically recorded and saved. These measures include the number of reward bottle activations (licks) and facial contact stimuli (face contacts), but custom measures like the lick/face ratio (total number of licks per session/total number of contacts) can also be created. The stimulus temperature can be set to a single temperature or multiple temperatures within a session. The OPAD is a high-throughput, easy to use operant assay which will lead to better translation of pain research in the future as it includes cortical input instead of relying on spinal reflex-based nociceptive assays.

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“…Meal patterns (i.e., food intake, meal size, meal number and meal duration) were calculated using Med Assoc. Inc. and proprietary software (Bellinger et al, 2007; Guan et al, 2005; Kerins et al, 2005; Kramer and Bellinger, 2009; Kramer et al, 2010). Meal duration has been shown to be a continuous, non-invasive measure of orofacial nociception (surface and deep) in undisturbed male and female rats (Kerins et al, 2005; Kerins et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

Kramer

Bellinger

2014

Neuroscience

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High levels of 17β-estradiol (E2) have been found to reduce inflammatory temporomandibular joint (TMJ) pain. A search for genes effected by a high concentration of estradiol showed an increase in GABAA receptor subunit α6 (Gabrα6) in the trigeminal ganglia (TG). Blockade of Gabrα6 expression in the TG increases masseter muscle nociception in male rats, but the relationship between estradiol’s effect on nociception and Gabrα6 expression remains unclear in females. To address this knowledge gap we hypothesized that reducing Gabrα6 expression in the TG will increase the orofacial nociceptive response of ovariectomized female rats treated with estradiol. To administer hormone osmotic pumps were placed in rats that dispensed a low diestrus plasma concentration of 17β-estradiol, in addition, 17β-estradiol was injected to produce a high proestrus plasma concentration of estradiol. A ligature was then placed around the masseter tendon to induce a nociceptive response; a model for TMJ muscle pain. Gabrα6 siRNA was later infused into the TG and the nociceptive response was measured using von Frey filaments and a meal duration assay. GABAA receptor expression was measured in the TG and trigeminal nucleus caudalis and upper cervical region (Vc-C1). Ligature significantly increased the nociceptive response but a high proestrus concentration of 17β-estradiol attenuated this response. Gabrα6 siRNA infusion decreased Gabrα6 expression in the TG and Vc-C1 but increased the nociceptive response after 17β-estradiol treatment. The results suggest estradiol decreased the orofacial nociceptive response, in part, by causing an increase in Gabrα6 expression.

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Specificity of meal pattern analysis as an animal model of determining temporomandibular joint inflammation/pain

Cited by 28 publications

References 29 publications

Capping a Pulpotomy with Calcium Aluminosilicate Cement: Comparison to Mineral Trioxide Aggregates

Capping a Pulpotomy with Calcium Aluminosilicate Cement: Comparison to Mineral Trioxide Aggregates

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol

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