Specificity of cyclopropane fatty acid synthesis in Escherichia coli. Utilization of isomers of monounsaturated fatty acids

Marinari, Lawrence A.; Goldfine, Howard; Panos, Charles

doi:10.1021/bi00706a030

Cited by 24 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cyclopropyl fatty acids are formed exclusively by transmethylation of a cis-monoenoic fatty acid esterified to a phospholipid (Law 1971, Marinari et al 1974). An increase in cyclopropyl acids relative to their precursors occurs as cultures enter stationary phase (Law et al 1963), as pH of the medium decreases (Buist & Findlay 19851, under low oxygen tension, high temperature, and high concentration of magnesium ions (Law 1971).…”

Section: Lipidsmentioning

confidence: 99%

Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow

Dobbs¹,

Guckert²

1988

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Biochemical and traditional methods were used to determine the influence of Caflianassa trilobata on microbiological and meiofaunal communities within its large, hghly consolidated burrow. Sediment was collected from the lining of the shrimp's burrow, the burrow matrix, and ambient, subsurface sediment. The lining and matrix were composed of poorly sorted, fine-grained material compared to sandy ambient sediment. Meiofauna, predominantly nematodes, were most abundant in ambient sediment, not in the burrow as has been found for other species of macrofauna. Concentrations of chlorophyll a were very high in the lining, consistent with the suggestion that C. trilobata lines its burrow walls with seagrass. Analyses of lipids indicated that relative to the matrix and ambient sediment, the lining abounds with pro-and eukaryotic biomass. Thirty-four phospholipid, ester-linked fatty acids (PLFA) were identified and quantified. Fatty acids were assigned to functional groups of microorganisms to assess spatial variations in the absolute abundance and relative proportions of microbial populations. Dominance of prokaryotes was pronounced in all 3 areas, especially the matrix. The lining was the most aerobic location, but anaerobic microhabitats simultaneously allowed proliferation of sulfate-reducing bacteria, e. g. Desulfobacter. A radial trend was detected in the ratio of bacteria with terminally branched PLFA, typical of Gram-positive organisms, to those with unsaturated PLFA common to Gram-negative bacteria. The ratio increased from lining to matrix to ambient sediment. The ratios of tram to cls isomers of 16:lw7 indicated that prokaryotes in the matrix were starved. Through construction and maintenance of its burrow, C. trilobata determines not only the absolute and relative abundances of microbes in its sedimentary environment, but their physiologic state as well.

show abstract

Section: Lipidsmentioning

confidence: 99%

Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow

Dobbs¹,

Guckert²

1988

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

show abstract

“…In general, most long-chain fatty acids having a hydrocarbon chain with a steric disorder in the central portion ofthe chain will suffice. Monounsaturated fatty acids with cis double bonds ranging from the 5 to the 13 position support growth, as will polyunsaturated fatty acids (21,26,28,29,69,81,100,103). Various cyclopropane fatty acids (81,100), branched-chain fatty acids (99), brominated derivatives of saturated fatty acids (27,32), and trans unsaturated fatty acids (26,28,29,81,(99)(100)(101)103) will also support growth.…”

Section: E Coli Fatty Acid Auxotrophsmentioning

confidence: 99%

Physical properties of membrane lipids: biological relevance and regulation.

Cronan¹,

Gelmann²

1975

Bacteriological Reviews

264

View full text Add to dashboard Cite

“…However, in the higher basidiomycetes, the unsaturated linoleic acid is the major constituent of fatty acids (Solberg 1989;Bonzom et al 1999;Sakai and Kajiwara 2004). The related oleic acid is shown in E. coli to be a substrate for the action of CFA synthase (Marinari et al 1974) and preliminary expression studies suggest that the E. coli and C. cinerea enzymes are at least partially interchangeable in function (S. Loos, M. Aebi and U. Kü es, unpublished results).…”

mentioning

confidence: 99%

An Essential Gene for Fruiting Body Initiation in the Basidiomycete Coprinopsis cinerea Is Homologous to Bacterial Cyclopropane Fatty Acid Synthase Genes

et al. 2006

View full text Add to dashboard Cite

The self-compatible Coprinopsis cinerea homokaryon AmutBmut produces fruiting bodies without prior mating to another strain. Early stages of fruiting body development include the dark-dependent formation of primary hyphal knots and their light-induced transition to the more compact secondary hyphal knots. The AmutBmut UV mutant 6-031 forms primary hyphal knots, but development arrests at the transition state by a recessive defect in the cfs1 gene, isolated from a cosmid library by mutant complementation. A normal primordia phenotype was achieved when cfs11 was embedded at both sides in at least 4.0 kb of native flanking DNA. Truncations of the flanking DNA lead to reduction in transformation frequencies and faults in primordia tissue formation, suggesting that the gene is also acting at later stages of development. The cfs1 gene encodes a protein highly similar to cyclopropane fatty acid synthases, a class of enzymes shown in prokaryotes and recently in a plant to convert membrane-bound unsaturated fatty acids into cyclopropane fatty acids. In C. cinerea 6-031, the mutant cfs1 allele carries a T-to-G transversion, leading to an amino acid substitution (Y441D) in a domain suggested to be involved in the catalytic function of the protein and/or membrane interaction.

show abstract

Specificity of cyclopropane fatty acid synthesis in Escherichia coli. Utilization of isomers of monounsaturated fatty acids

Cited by 24 publications

References 34 publications

Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow

Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow

Physical properties of membrane lipids: biological relevance and regulation.

An Essential Gene for Fruiting Body Initiation in the Basidiomycete Coprinopsis cinerea Is Homologous to Bacterial Cyclopropane Fatty Acid Synthase Genes

Contact Info

Product

Resources

About