Specificity of antigens from pathogenicAspergillusspecies

Magaldi, Sylvia; Mackenzie, D. W. R.

doi:10.1080/00362178485380631

Cited by 19 publications

(12 citation statements)

References 20 publications

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“…The shorter shelf life for the reagent is also a major factor prohibiting its widespread use. In recent years the ELISA technique has been used to detect the antibody classes in the sera of patients [5,14,21,24,27]. However, the sensitivity of this method is not quite comparable to the radioimmunoassay methods.…”

Section: Discussionmentioning

confidence: 99%

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A Partially Purified Glycoprotein Antigen from Aspergillus fumigatus

Kurup¹,

John²,

Resnick³

et al. 1986

Int Arch Allergy Immunol

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Culture filtrate antigens of Aspergillus fumigatus were fractionated by isoelectric focusing using a pH gradient of 4–6.5. Three fractions, namely, 18, 19 and 20 were pooled and subjected to immunochemical analysis. It contained a concanavalin A binding glycoprotein. Antibody raised against this component was used to prepare an affinity column of IgG-Sepharose and was used to purify crude culture filtrate antigens. This component produced three precipitin arcs in crossed immunoelectrophoresis using anti-A. fumigatus rabbit serum. This fraction has an isoelectric point of 6.5 and showed three components in two-dimensional electrophoresis with approximate molecular weights of 20, 40 and 80 kilo daltons. This antigen reacted with patient sera in the biotinavidin linked immunosorbent assay and showed high levels of anti-A. fumigatus IgG and IgE antibodies in allergic bronchopulmonary aspergillosis and IgG antibodies in aspergilloma. Both controls and Aspergillus skin test positive asthmatics showed only low levels of specific antibodies. Because of the purity of this antigen, its potential use as a standardized antigen in the detection of antibody is discussed.

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Section: Discussionmentioning

confidence: 99%

“…The crude preparations obtained either from the cytosol or culture filtrate usually contain over 30 components in antigen antibody crossed immunoe lectrophoresis and two-dimensional electrophoresis [5,9,11,19]. The presence of these components may vary from strain to strain and in different prepara tions from a single strain.…”

Section: Introductionmentioning

confidence: 99%

A Partially Purified Glycoprotein Antigen from Aspergillus fumigatus

Kurup¹,

John²,

Resnick³

et al. 1986

Int Arch Allergy Immunol

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“…The second-dimension gel run utilized anti-A, corymbifera antiserum 1:10 (v/v, antiserum to gel). i and 2, immunoprecipitates; P, primary gel; S, spacer gel; A, antibody containing gel; W, well for antigen Immunological cross-reactivity is marked between species of Aspergillus (De Magaldi and Mackenzie, 1984;Jensen and Schcnheyder, 1989) and among the genera causing zygomycosis (Hessian and Smith, 1982;Jones and Kaufman, 1978); positive immunoprecipitation in XIE, therefore, should be regarded as diagnostic only to the level of aspergillosis and zygomycosis.…”

Section: Apaap Techniquementioning

confidence: 97%

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Jensen

1993

Vet Res Commun

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A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens of Aspergillus fumigatus and Absidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negative. The method worked with unabsorbed sera, was specific, and required only standard electrophoretic equipment. It can therefore supplement chemical detection of fungi in tissues in the diagnosis of bovine aspergillosis and zygomycosis.

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“…In contrast, cross-reactions were common when standard antisera prepared in animals against heterologous species of Aspergillus were tested against A. fumigatus antigens. Lines of identity between homologous antigens and those from A. fumigatus were observed in 5 of 9 lines obtained with A. flavus, 4 of 16 lines of A. nidulans, 4 of 9 lines of A. niger and 4 of 8 lines of A. terreus.In a previous report [5], the specificities of a range of antigens obtained from Aspergillus fumigatus and four other pathogenic Aspergillus species (tt. flavus, A. nidulans, A. niger and A. terreus) were compared by ELISA and immunofluorescence procedures.…”

mentioning

confidence: 99%

“…In a previous report [5], the specificities of a range of antigens obtained from Aspergillus fumigatus and four other pathogenic Aspergillus species (tt. flavus, A. nidulans, A. niger and A. terreus) were compared by ELISA and immunofluorescence procedures.…”

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confidence: 99%

Specificity of antigens from pathogenicAspergillusspecies

Magaldi¹,

Mackenzie²

1984

Med Mycol

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Somatic (mycelial) and metabolic (culture filtrate) antigens of Aspergillusflavus, A. fumigatus, A. nidulans, A. niger and A. terreus were compared by line immunoelectroplioresis with sera from patients with allergic broncliopulmonary aspergillosis (ABPA) or aspergilloma, or from immunized animals. Number of lines observed when tested with human sera were similar for somatic and metabolic preparations of A. fumigatus, but up to 33 lines were present when both types of antigens were tested simultaneously. Cross-reactions between heterologous antigens and sera from patients with aspergiUoma or ABPA were uncommon. In contrast, cross-reactions were common when standard antisera prepared in animals against heterologous species of Aspergillus were tested against A. fumigatus antigens. Lines of identity between homologous antigens and those from A. fumigatus were observed in 5 of 9 lines obtained with A. flavus, 4 of 16 lines of A. nidulans, 4 of 9 lines of A. niger and 4 of 8 lines of A. terreus.In a previous report [5], the specificities of a range of antigens obtained from Aspergillus fumigatus and four other pathogenic Aspergillus species (tt. flavus, A. nidulans, A. niger and A. terreus) were compared by ELISA and immunofluorescence procedures. This second report describes the results of studies on shared and specific antigens within pathogenic Aspergillus spp. as revealed by line immunoelectrophoresis (LIE). METHODS AntigensOne mycelial (somatic) extract of A. fumigatus containing water soluble (WS) components has been described previously [5], viz. WS2140/2, prepared from isolate NCPF 2140. A culture filtrate (metabolic) antigen (M 0608) was prepared from isolate IND 0608 grown at 37"C for 35 days in continuously stirred glucose peptone broth [2]. Mycelial growth was removed by centrifugation. The culture medium was then dialysed against distilled water for 72 h and freeze dried. ~Present address:

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Specificity of antigens from pathogenicAspergillusspecies

Cited by 19 publications

References 20 publications

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Specificity of antigens from pathogenicAspergillusspecies

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