Specificity and Sensitivity Characterization of a Gallium Arsenide Resonant Bio-Sensor

Chawich, Juliana; Boiteux, Pierre; Leblois, Thérèse; Élie-Caille, Céline

doi:10.1109/ultsym.2018.8579803

Cited by 2 publications

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“…The compatibility of this material with many chemical functionalization approaches and surface micro/nanofabrication processes makes it an ideal candidate for a biosensor application [ 23 , 24 ]. The surface of GaAs can be chemically functionalized with alkanethiols [ 25 , 26 , 27 , 28 ], silanes and phosphonates [ 29 ], and can be relatively easily regenerated [ 30 , 31 ], which gives this material very attractive functionalities for the fabrication of antibody-based architectures. Furthermore, it has been reported that an enhanced piezoelectric response of the GaAs-based acoustic sensor could be achieved upon deposition of a thin film of ZnO [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium

et al. 2021

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A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of Escherichia coli in liquid media. The geometry of the biosensor consists of a GaAs membrane coated with a thin film of piezoelectric ZnO on its top surface. A pair of electrodes deposited on the ZnO film allows the generation of BAWs by lateral field excitation. The back surface of the membrane is functionalized with alkanethiol self-assembled monolayers and antibodies against E. coli. The antibody immobilization was investigated as a function of the concentration of antibody suspensions, their pH and incubation time, designed to optimize the immunocapture of bacteria. The performance of the biosensor was evaluated by detection tests in different environments for bacterial suspensions ranging between 103 and 108 CFU/mL. A linear dependence between the frequency response and the logarithm of E. coli concentration was observed for suspensions ranging between 103 and 107 CFU/mL, with the limit of detection of the biosensor estimated at 103 CFU/mL. The 5-fold regeneration and excellent selectivity towards E. coli detected at 104 CFU/mL in a suspension tinted with Bacillus subtilis at 106 CFU/mL illustrate the biosensor potential for the attractive operation in complex biological media.

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