Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Wu, Zhongming; Drayton, Danielle L.; Pisetsky, D S

doi:10.1046/j.1365-2249.1997.4301328.x

Cited by 32 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, normal human subjects (NHS) can express antibodies specific for some bacterial DNA antigens. These findings are consistent with the recognition of backbone determinants by lupus anti-DNA and sequences by NHS anti-DNA [17, 18]. …”

Section: Resultssupporting

confidence: 89%

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

Wang

Stearns

et al. 2014

Clinical Immunology

View full text Add to dashboard Cite

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE). To elucidate specificity further, the effect of polyamines on the binding of anti-DNA antibodies from patients with lupus was tested by ELISA to calf thymus (CT) DNA; we also assessed the binding of plasmas of patients and normal human subjects (NHS) to Micrococcus luteus (MC) DNA. As these studies showed, spermine can dose-dependently inhibit SLE anti-DNA binding to CT DNA and can promote dissociation of preformed immune complexes. With MC DNA as antigen, spermine failed to inhibit the NHS anti-DNA binding. Studies using plasmas adsorbed to a CT DNA cellulose affinity indicated that SLE plasmas are mixtures of anti-DNA that differ in inhibition by spermine and binding to conserved and non-conserved determinants. Together, these studies demonstrate that spermine can influence the binding of anti-DNA autoantibodies and may contribute to the antigenicity of DNA.

show abstract

Section: Resultssupporting

confidence: 89%

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

Wang

Stearns

et al. 2014

Clinical Immunology

View full text Add to dashboard Cite

show abstract

“…In this context, an association between S. aureus and human lupus has been documented in many reports. Lupus patients have high levels of antibodies to staphylococcal lipoteichoic acid (58) and staphylococcal DNA (59). Induction or flare of SLE and cutaneous lesions has been described following an infection with S. aureus (60).…”

Section: Discussionmentioning

confidence: 99%

Chronic Exposure to Staphylococcal Superantigen Elicits a Systemic Inflammatory Disease Mimicking Lupus

Chowdhary

Tilahun

Clark

et al. 2012

The Journal of Immunology

View full text Add to dashboard Cite

Chronic nasal and skin colonization with superantigen (SAg)-producing Staphylococcus aureus is well documented in humans. Given that trans-mucosal and trans-cutaneous absorption of SAgs can occur, we determined whether chronic exposure to small amounts of SAg per se could activate autoreactive CD4+ and CD8+ T cells and precipitate any autoimmune disease without further external autoantigenic stimulation. Because HLA class II molecules present SAg more efficiently than do mouse MHC class II molecules, HLA-DQ8 transgenic mice were implanted s.c. with mini-osmotic pumps capable of continuously delivering the SAg, staphylococcal enterotoxin B (total of 10 μg/mouse), or PBS over 4 wk. Chronic exposure to staphylococcal enterotoxin B resulted in a multisystem autoimmune inflammatory disease with features similar to systemic lupus erythematosus. The disease was characterized by mononuclear cell infiltration of lungs, liver, and kidneys, accompanied by the production of anti-nuclear Abs and deposition of immune complexes in the renal glomeruli. The inflammatory infiltrates in various organs predominantly consisted of CD4+ T cells bearing TCR Vβ8. The extent of immunopathology was markedly reduced in mice lacking CD4+ T cells and CD28, indicating that the disease is CD4+ T cell mediated and CD28 dependent. The absence of disease in STAT4-deficient, as well as IFN-γ–deficient, HLA-DQ8 mice suggested the pathogenic role of Th1-type cytokines, IL-12 and IFN-γ. In conclusion, our study suggests that chronic exposure to extremely small amounts of bacterial SAg could be an etiological factor for systemic lupus erythematosus.

show abstract

“…DNA antigen for the ELISA was purified from salmon testis DNA and pCMV‐lacZ by alcohol extraction 16 and then treated either with S 1 nuclease to give dsDNA or by boiling to give ssDNA 16 . Fish antibodies to ds plasmid DNA, ds salmon testis DNA and ss salmon testis DNA were detected by ELISA 16 using mouse monoclonal antibody WCI 12. Positive antibodies to DNA were from patients with systemic lupus erythematosus (SLE) (Inova Diagnostics, San Diego, CA) and their binding was detected by a rabbit anti‐human peroxidase‐conjugate (Dako, Copenhagen).…”

Section: Methodsmentioning

confidence: 99%

The safety and longevity of DNA vaccines for fish

et al. 1999

View full text Add to dashboard Cite

A plasmid that contained the cytomegalovirus (CMV)-promoter-driven lacZ reporter gene (pCMV-lacZ) remained in the epaxial muscle of five of eight goldfish as covalently closed circles, the most functional form of plasmid, for at least 70 days at 22 degrees. It was not present in the gills or elsewhere by polymerase chain reaction and was not integrated. Its expressed protein, Escherichia coli beta-galactosidase (beta-gal), which was in the injected myofibres, was detected in all the fish at 4-21 days and in about half the fish from 28 days until the end of the experiment at 70 days. The numbers of cells that secreted antibody to beta-gal in the kidney peaked at 14 days. Serum antibody and proliferating kidney cells to beta-gal were in all fish from 14 days with a plateau of the responses from 21 days onwards. The plasmid did not induce autoimmune-like antibodies to itself or to single- or double-stranded salmon testis DNA. Plasmids can therefore induce long-term foreign protein expression whilst inducing humoral and cell-mediated immunity without autoimmunity or integration in goldfish.

show abstract

Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE)

Cited by 32 publications

References 13 publications

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

Chronic Exposure to Staphylococcal Superantigen Elicits a Systemic Inflammatory Disease Mimicking Lupus

The safety and longevity of DNA vaccines for fish

Contact Info

Product

Resources

About