Specificity and Affinity of BPTF PHD Finger and Bromodomain in the Context of the Nucleosome

Abstract

acetylation, methylation and ubiquitination. Post-translational modifications can regulate chromatin processes through the recruitment of effector proteins or by directly impacting the compaction and dynamics of the chromatin polymer. Here, we study the impact of histone H4K20 methylation on chromatin compaction using solution and solid-state NMR spectroscopy. In particular, we compare the nucleosome interactions and dynamics of semi-synthetic chromatin arrays with and without methylation. Our strategy provides valuable information regarding chromatin structure in a regime currently inaccessible by other structural techniques, including cryo-electron microscopy and singlemolecule biophysical approaches.