Specification of the effect of chelating complex of iron ions in androgenesisin vitro by means of cation-free minimal medium

Vagera, J.; Jı́lek, Milan

doi:10.1007/bf02902276

Cited by 9 publications

(1 citation statement)

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“…Thus, only glycine, inositol and sucrose are probably not affected by AC. Data presented here show however that developing embryos in anther cultures of Anemone canadensis are not dependent on the presence of organic substances other than Fe-EDTA (and very probably sucrose; this was however not tested), thereby confirming results on tobacco of and Vagera and Jilek (1984). It is likely that requirements for amino acids, vitamins and plant growth regulators are fulfilled by the levels present in the anthers themselves.…”

Section: Discussionsupporting

confidence: 74%

Correlations between activated charcoal, Fe‐EDTA and other organic media ingredients in cultured anthers of Anemone canadensis

Johansson

Calleberg

Gedin

1990

Physiologia Plantarum

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The effects of activated charcoal and organic substances on embryogenesis in anther cultures of Anemone canadensis L. were studied. Embryogenesis was independent of the presence in the culture medium of glycine, nicotinic acid, pyridoxine, thiamine, folic acid, d‐biotin or myoinositol. Absence of Fe‐EDTA totally inhibited embryogenesis. Activated charcoal (AC) adsorbed Fe‐EDTA, pyridoxine, folic acid and nicotinic acid in a double‐layer medium almost completely within 24 h. If petri dishes according to the souble‐layer method were stored overnight or more, embryogenesis was totally inhibited, probably due to adsorbtion of Fe‐EDTA by AC. It was shown that AC itself released some yet unidentified substance(s) that stimulated embryogenesis. The addition of polyvinylpolypyrrolidone (PVPP) to the culture medium stimulated embryogenesis, but PVPP was not as efficient as AC. Embryogenesis was totally inhibited when AC and PVPP were applied together. Minor additions of ethanol to the culture medium stimulated embryogenesis when AC was present, but no such effect was obtained when AC was absent.

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Section: Discussionsupporting

confidence: 74%