Specification of human regional brain lineages using orthogonal gradients of WNT and SHH in organoids
Soraya Scuderi,
Tae-Yun Kang,
Alexandre Jourdon
et al.
Abstract:The repertory of neurons generated by progenitor cells depends on their location along antero-posterior and dorso-ventral axes of the neural tube. To understand if recreating those axes was sufficient to specify human brain neuronal diversity, we designed a mesofluidic device termed Duo-MAPS to expose induced pluripotent stem cells (iPSC) to concomitant orthogonal gradients of a posteriorizing and a ventralizing morphogen, activating WNT and SHH signaling, respectively. Comparison of single cell transcriptomes… Show more
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