Specification of cell fates within the salivary gland primordium

Haberman, Adam; Isaac, Daniel D.; Andrew, Deborah J.

doi:10.1016/s0012-1606(03)00140-4

Cited by 47 publications

(58 citation statements)

References 49 publications

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“…In control salivary glands, GFP-actin marks basolateral and apical membranes of epithelial cells and outlines the lumen of the gland (Fig. 3C), in agreement with previous reports in references [51][52][53]. The epithelial cells are columnar and elongated in the basal to apical direction, with a centrally localized nucleus.…”

Section: Resultssupporting

confidence: 91%

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

Szul¹,

Burgess

Jeon

et al. 2011

Cellular Logistics

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Section: Resultssupporting

confidence: 91%

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

Szul¹,

Burgess

Jeon

et al. 2011

Cellular Logistics

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“…In addition to these positive roles, FKH also represses the expression of salivary ductspecific genes in the secretory cells (Haberman et al, 2003). Here, we discover yet another role for fkh in the salivary gland: the maintenance of SPCG expression.…”

Section: Direct Regulation Of Spcgs By Crebamentioning

confidence: 74%

CrebA regulates secretory activity in theDrosophilasalivary gland and epidermis

2005

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Understanding how organs acquire the capacity to perform their respective functions is important for both cell and developmental biology. Here, we have examined the role of early-expressed transcription factors in activating genes crucial for secretory function in the Drosophila salivary gland. We show that expression of genes encoding proteins required for ER targeting and translocation, and proteins that mediate transport between the ER and Golgi is very high in the early salivary gland. This high level expression requires two early salivary gland transcription factors; CrebA is required throughout embryogenesis and Fkh is required only during late embryonic stages. As Fkh is required to maintain late CrebA expression in the salivary gland, Fkh probably works through CrebA to affect secretory pathway gene expression. In support of these regulatory interactions, we show that CrebA is important for elevated secretion in the salivary gland. Additionally, CrebA is required for the expression of the secretory pathway genes in the embryonic epidermis, where CrebA had previously been shown to be essential for cuticle development. We show that zygotic mutations in several individual secretory pathway genes result in larval cuticle phenotypes nearly identical to those of CrebA mutants. Thus, CrebA activity is linked to secretory function in multiple tissues.

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“…Scr acts as a master control regulator of salivary gland morphogenesis during embryonic development (20)(21)(22)(23)(24)(25). When expressed throughout the embryo, Scr is able to induce an additional pair of salivary glands anterior to parasegment 2 (20), where the normal salivary glands form.…”

Section: Resultsmentioning

confidence: 99%

Function and specificity of synthetic Hox transcription factors in vivo

Papadopoulos

Vukojević

Adachi

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Homeotic (Hox) genes encode transcription factors that confer segmental identity along the anteroposterior axis of the embryo. However the molecular mechanisms underlying Hox-mediated transcription and the differential requirements for specificity in the regulation of the vast number of Hox-target genes remain ill-defined. Here we show that synthetic Sex combs reduced (Scr) genes that encode the Scr C terminus containing the homedomain (HD) and YPWM motif (Scr-HD) are functional in vivo. Synthetic Scr-HD peptides can induce ectopic salivary glands in the embryo and homeotic transformations in the adult fly, act as transcriptional activators and repressors during development, and participate in protein-protein interactions. Their transformation capacity was found to be enhanced over their full-length counterpart and mutations known to transform the full-length protein into constitutively active or inactive variants behaved accordingly in the synthetic peptides. Our results show that synthetic Scr-HD genes are sufficient for homeotic function in Drosophila and suggest that the N terminus of Scr has a role in transcriptional potency, rather than specificity. We also demonstrate that synthetic peptides behave largely in a predictable way, by exhibiting Scr-specific phenotypes throughout development, which makes them an important tool for synthetic biology.synthetic genes | transcriptional specificity | Hox genes | Sex combs reduced | homeotic transformations H omeotic genes code for transcription factors that play an instrumental role in animal development by specifying the identity of body segments along the anteroposterior axis of the embryo (1-4). Hox genes have persisted in the animal kingdom; they are found in animals as diverse as worms and humans (5, 6) and the Homeodomain (HD), a helix-turn-helix DNA-binding domain, has been strikingly conserved in animals since before the bilaterian split (1,7,8). Sequence-specific binding of Hox proteins has been studied for the Drosophila Sex combs reduced (Scr) (9, 10), Antennapedia (Antp) (11, 12) and Ultrabithorax (Ubx) (11, 12) HDs. A consensus sequence TAATC/GC/G recognition core was identified in all of them, which alone is obviously not sufficient to confer transcriptional specificity, because it occurs statistically every kilobase in the genome. Similar sequence preferences have been identified for Deformed (Dfd) and Abdominal-B (Abd-B) (11), raising the question of how target specificity is achieved among different Hox paralogs.A closer look into conserved residues outside the HD identified its amino-terminal YPWM motif that is present in almost all Hox proteins, from flies to vertebrates [with the exception of Abdominal-B (Abd-B), which has conserved only the tryptophan at position 3] (13). Extradenticle (Exd) and its mammalian homolog Pbx1 (14) were found to interact specifically with the YPWM motif of Hox proteins in vitro (15) and crystallographic analysis of a Ubx-Exd complex determined the topology of this interaction (16). Recently the link between the Ant...

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Specification of cell fates within the salivary gland primordium

Cited by 47 publications

References 49 publications

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

The Garz Sec7 domain guanine nucleotide exchange factor for Arf regulates salivary gland development in Drosophila

CrebA regulates secretory activity in theDrosophilasalivary gland and epidermis

Function and specificity of synthetic Hox transcription factors in vivo

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