Specific V gene usage in anti-phosphorylcholine ige antibodies

Lötscher, Marcel; Blaser, Kurt; Heusser, Christoph

doi:10.1016/0161-5890(93)90448-k

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…9,10 Despite extensive research concerning the regulatory mechanism involved in specific IgE formation, only a few studies have dealt with the specific V-gene usage in IgE antibodies. [11][12][13] We report here the cDNA cloning and sequence analysis of the variable region genes of four IgE and one IgG1 antibodies from the same group. The other IgE antibody, 4G8, was found to share identical sequences with the IgE antibody TE2 in both V H and V L genes.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the V regions of four IgE antibodies specific for trichosanthin

Wang

Yeh

1996

Immunology

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SUMMARYWe report here the cDNA cloning and variable region nucleic acid sequences of the heavy and the light chains of four monoclonal IgE and one monoclonal IgG1 antibodies against trichosanthin (TCS), all of which recognized a similar or related antigenic determinant. Sequence analysis revealed that the V H regions of the four IgE antibodies use different V gene segments from the 7183, 36-60 and J558 families. The D H genes varied extensively in nucleotide sequence and length, with D segments DSP2, DFL16 and DQ52 being used with equal frequency. All J H gene segments except J H 1 were present in the e transcripts. In contrast, there appeared to be restricted L-chain utilization. All four L-chain V genes cloned belonged to the Vk21 family and a bias in the use of Jk1 gene was also observed. The V L sequences of the anti-TCS monoclonal antibodies showed much less diversity than the V H sequences. We propose that the L-chain rather than H-chain may play a more significant role in specific binding with allergenic determinant on TCS.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of the V regions of four IgE antibodies specific for trichosanthin

Wang

Yeh

1996

Immunology

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“…It is possible that the aromatic residue used for coupling the hapten to the carrier protein might be the complementary structure eliciting the specificity of these antibodies. It has been described that BALB/c mice immunized with PC-KLH induce IgE production capable of recognizing the integral hapten, which necessarily includes the nitrophenyl group [37,38]. IgE antibody specificity to the nitrophenyl portion was controlled by high mutation rates at the genomic sequences in other anti-PC antibodies, or by the use of genetic combinations in antibodies that react exclusively with the nitrophenyl portion [38].…”

Section: Discussionmentioning

confidence: 99%

“…It is also possible that the females develop antibodies with a greater reactivity to the incomplete hapten (PC), or due to a restriction of this reactivity in the male response. The preferential reactivity of anti-PC antibodies to PC or NPPC involves the use of genomic combinations of heavy chain variable segments in BALB/c mice [38]. Consideration should also be given to whether the females are capable of producing antibodies that can mediate anaphylaxis by activating macrophages through FcγRIII.…”

Section: Discussionmentioning

confidence: 99%

Cross-reactivity of anti-phosphorylcholine antibodies to neuromuscular blockers in a murine model of immunization

Mazzuco

Sato

Vasconcelos

et al. 2007

International Immunopharmacology

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Antigen dose‐dependent predominance of either direct or sequential switch‘qc in IgE antibody responses

1997

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Priming of CBA/J mice with minute doses of protein antigens (Ag) leads to high IgE antibody (Ab) titres in the immune sera of these animals. In contrast priming with large doses elicits only a marginal production of IgE Ab. In vitro restimulation of spleen cells from animals primed with large doses and lacking in vivo IgE Ab leads to a burst of IgE Ab-forming cells. This in vitro anamnestic response is lacking in mice primed with minute doses of Ag. In order to trace the cellular basis of the in vitro IgE memory response we have extended the analysis of the distribution of Ab isotypes to Ag-primed IgG1-deficient delta 5'S gamma 1 mice. The data presented here must be interpreted as followed. Priming of mice with minute doses of Ag leads to a direct switch from IgM to IgE Ab expression in both strains. These animals have high IgE Ab titres without establishing an IgE memory. The direct switch was verified by polymerase chain reaction and Southern blot analysis of switch circle DNA isolated from Ag-specific B cells of CBA/J mice primed with minute doses of Ag. In contrast to immunization with minute doses, priming with large doses of Ag fails to induce in vivo IgE Ab production in CBA/J and delta 5'S gamma 1 mice but establishes a B epsilon memory in CBA/J mice which involves IgG1-bearing intermediate B cells. In vivo these B epsilon memory cells do not enter the status of IgE Ab-producing cells. In vitro they can be released from this anergy and presumed suppression and develop in an anamnestic response into a large population of IgE Ab-forming B cells. This increase in the number of IgE Ab-producing cells after restimulation in vitro is lacking in delta 5'S gamma 1 mice, apparently because of their inability to generate IgG1-expressing precursor cells. The notion of a sequential switch and an IgG1 intermediate B epsilon memory status is also supported by depletion and inhibition experiments. Elimination of IgG1-expressing B cells in CBA/J mice primed with high doses of Ag prevents the IgE Ab burst after in vitro challenge with Ag. The data further suggest that the two switch pathways are not mutually exclusive and that the Ag dose can decide which pathway is preferentially used.

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Specific V gene usage in anti-phosphorylcholine ige antibodies

Cited by 3 publications

References 36 publications

Molecular characterization of the V regions of four IgE antibodies specific for trichosanthin

Molecular characterization of the V regions of four IgE antibodies specific for trichosanthin

Cross-reactivity of anti-phosphorylcholine antibodies to neuromuscular blockers in a murine model of immunization

Antigen dose‐dependent predominance of either direct or sequential switch‘qc in IgE antibody responses

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