Specific uptake kinetics of glucose and nitrate in carbon-limited and nitrogen-limited C:N ratio under photoheterotrophic cultural conditions for Botryococcus braunii growth and lipid production

Khichi, Shailendra Singh; Dohare, Deepak; Sangineni, Rohith; Sachin, Sharika; Ghosh, Sanjoy

doi:10.1016/j.biteb.2019.100337

“…An appropriate C/N ratio is the key to maximising the growth and lipids production capacity of strains. Khichi et al ( 2019 ) shown that Botryococcus braunii achieved the maximum biomass accumulation and lipids production when the C/N ratio was 29:1 (1.11 g/L/day and 0.390 g/L/day, respectively). Matsakas et al ( 2015 ) shown that an appropriate C/N ratio could stimulate lipids-producing microorganisms to accumulate more microbial lipids.…”

Section: Discussionmentioning

confidence: 99%

Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

Ye

¹

,

Sun

²

,

Hao

³

et al. 2021

View full text Add to dashboard Cite

Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.

show abstract

“…An appropriate C/N ratio is the key to maximising the growth and lipids production capacity of strains. (Khichi et al 2019) showed that Botryococcus braunii achieved the maximum biomass accumulation and lipids production when the C/N ratio was 29:1 (1.11 g/L/day and 0.390 g/L/day, respectively). (Matsakas et al 2015) showed that an appropriate C/N ratio can stimulate lipids-producing microorganisms to accumulate more microbial lipids.…”

Section: Discussionmentioning

confidence: 99%

Optimising Nutrients in the Culture Medium of Rhodosporidium Toruloides Enhances Lipids Production

Ye

¹

,

Sun

²

,

Hao

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.

show abstract

“…Otros factores reportados como críticos para la síntesis de hidrocarburos y lípidos son la salinidad, la temperatura, la nutrición, la turbidez, el OD, el CO 2 y el pH. [24,26,107]. La tabla 1 reúne las condiciones empleadas para la producción de 8 cepas de B. braunii ampliamente estudiadas.…”

Section: Hidrocarburos Y Biocombustiblesunclassified

Análisis De Las Aplicaciones De La Microalga Botryococcus Braunii En Procesos Industriales

Barajas-Solano

¹

2021

Cienc. En Desarro.

0

View full text Add to dashboard Cite

Las microalgas son microorganismos fotosintéticos los cuales pueden ser eucariotas (algas) o procariotas (cianobacterias) y pueden estar presentes en todos los hábitats de la tierra. Este artículo ofrece una descripción bibliográfica sobre análisis técnicos en la producción industrial de los principales metabolitos extraídos de Botryococcus braunii con el objetivo de analizar, determinar e identificar los avances científicos para la utilización de esta alga como un recurso potencial, por medio de una recopilación bibliométrica de 20 años sobre artículos de investigaciones demostrando gran interés en el campo de las microalgas. Se ha realizado este trabajo con el propósito de averiguar los estudios en el área de la microalga Botryococcus braunii y sus aplicaciones industriales, también determinar los principales metabolitos como hidrocarburos, exopolisacáridos, carotenoides, proteínas, en el aprovechamiento de procesos industriales como materiales, alimentos, productos farmacéuticos, nanopartículas y biocombustibles. Debido a sus vías metabólicas particulares, su adaptabilidad fisiológica y su uso en sistemas de cultivo avanzados, existe un gran potencial para producir sustancias químicas de alto valor a partir de cepas de Botryococcus braunii.

show abstract

Specific uptake kinetics of glucose and nitrate in carbon-limited and nitrogen-limited C:N ratio under photoheterotrophic cultural conditions for Botryococcus braunii growth and lipid production

Cited by 3 publications

References 28 publications

Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

Optimising Nutrients in the Culture Medium of Rhodosporidium Toruloides Enhances Lipids Production

Análisis De Las Aplicaciones De La Microalga Botryococcus Braunii En Procesos Industriales

Contact Info

Product

Resources

About