Specific transcription and reinitiation of 2,4-D-induced genes in tobacco nuclei

Mennes, A. M.; Quint, A.; Gribnau, Joost; Boot, C. J. M.; Zaal, E. J. van der; Maan, Arie C.; Libbenga, K. R.

doi:10.1007/bf00018461

Cited by 7 publications

(3 citation statements)

References 22 publications

(26 reference statements)

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“…Higher total RNA accumulation in C. vulgaris cells in response to IM is also consistent with observations that auxin application to whole plants, excised organs or cell cultures leads to a rapid increase in levels of numerous mRNAs (Raghavan et al 2006). In vitro transcription studies with isolated nuclei showed that accumulation of those mRNA species was due to enhanced transcription rates and 2,4-D-dependent stimulation of RNA polymerase II activity (Mennes et al 1992). …”

Section: Discussionsupporting

confidence: 65%

The effect of indomethacin on the growth and metabolism of green alga Chlorella vulgaris Beijerinck

et al. 2008

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The aim of the present work was to study the effect of indomethacin (IM), a pleiotropic therapeutic substance commonly used in animal systems, at concentration range of 10 -8 to 10 -3 M on the growth and metabolism of single-celled Chlorella vulgaris (Chlorophyceae). Because of the presence of the indole ring in its molecule, IM is characterized by structural similarity with natural auxins, e.g. IAA. It was found that IM influenced algal growth, macromolecular synthesis and metabolism in dosedependent manner. IM had the highest stimulating effect on algae at 10 -7 M on the 5th day of culture resulting in the increase in cell number and dry mass, DNA, RNA, proteins, phosphates, monosaccharides, photosynthetic pigments and glycolic acid content as well as protein extracellular secretion to the environment. Specific proteins from the region 20-139 kDa appeared during 10 -7 M IM treatment on the 5th day of cultivation as analysed by SDS-PAGE. IMinduced photosynthetic oxygen exchange in green alga was also noted. In contrast, the treatment with IM at the highest concentration of 10 -3 M suppressed cell division, dry mass production and decreased the level of the analysed parameters during the whole 7-day period of cultivation. Therefore, it could be speculated that IM functioned as a plant growth regulator affecting cell division and metabolism of green alga C. vulgaris.

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Section: Discussionsupporting

confidence: 65%

The effect of indomethacin on the growth and metabolism of green alga Chlorella vulgaris Beijerinck

et al. 2008

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“…The elution profile of 3H-UMP-labelled in vitro transcripts from the mercury Sepharose affinity column was very similar to what has been reported for animal [3], yeast [4] and other plant [8] nuclei (Fig. 1A).…”

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confidence: 62%

“…To circumvent this problem, the successful use of fl-thio nucleoside triphosphates has been reported [ 12]. Although affinity labelling of RNA has also been described in plant nuclei [5,8], the possibility of post-transcriptional transfer of the 7-thiol group has not been examined in detail. From the data presented here it is clear that inhibition of signals by heparin does not prove reinitiation.…”

mentioning

confidence: 99%

Post-transcriptional transfer of ?-thio affinity label to RNA in isolated parsley nuclei

Schweizer

Hahlbrock

1993

Plant Mol Biol

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As an alternative to soluble plant transcription systems, we examined the reinitiation capacity of isolated parsley nuclei. Nuclear chalcone synthase in vitro transcripts were affinity-labelled with gamma-thio-ATP, gamma-thio-GTP or beta-thio-ATP, and purified by chromatography on a mercury Sepharose affinity column. Primer extension and subsequent PCR amplification of these in vitro transcripts revealed gamma-thio-ATP-dependent, but no beta-thio-ATP-dependent, signals, although affinity labelling of overall in vitro transcripts still occurred with beta-thio-ATP. We conclude that the described plant nuclei reinitiated transcription non-specifically and that post-transcriptional transfer of the gamma-thio affinity label severely interfered with the detection of reinitiated transcripts.

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In Vitro Transcription of Plant Nuclear Genes

Schweizer

1994

Results and Problems in Cell Differentiation

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Specific transcription and reinitiation of 2,4-D-induced genes in tobacco nuclei

Cited by 7 publications

References 22 publications

The effect of indomethacin on the growth and metabolism of green alga Chlorella vulgaris Beijerinck

The effect of indomethacin on the growth and metabolism of green alga Chlorella vulgaris Beijerinck

Post-transcriptional transfer of ?-thio affinity label to RNA in isolated parsley nuclei

In Vitro Transcription of Plant Nuclear Genes

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