Specific total starch determinations in conifer tissues with glucose oxidase

Ebell, L. F.

doi:10.1016/s0031-9422(00)85790-8

Cited by 43 publications

(30 citation statements)

References 26 publications

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“…The starch was digested to glucose for 30rain at 55 °C with amyloglucosidase from Asperillus oryzae (Sigma A-9268, grade V, dialyzed and diluted to 10unitsm1-1 in 50mM Na-acetate buffer, pH 4.5). Glucose concentrations were determined using a glucose oxidase-iodide assay [6].…”

Section: Methodsmentioning

confidence: 99%

Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide

1985

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The effect of long-term exposure to elevated levels of CO2 on biomass partitioning, net photosynthesis and starch metabolism was examined in cotton. Plants were grown under controlled conditions at 350, 675 and 1000 μl l(-1) CO2. Plants grown at 675 and 1000 μl l(-1) had 72% and 115% more dry weight respectively than plants grown at 350 μl l(-1). Increases in weight were partially due to corresponding increases in leaf starch. CO2 enrichment also caused a decrease in chlorophyll concentration and a change in the chlorophyll a/b ratio. High CO2 grown plants had lower photosynthetic capacity than 350 μl l(-1) grown plants when measured at each CO2 concentration. Reduced photosynthetic rates were correlated with high internal (non-stomatal) resistances and higher starch levels. It is suggested that carbohydrate accumulation causes a decline in photosynthesis by feedback inhibition and/or physical damage at the chloroplast level.

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Section: Methodsmentioning

confidence: 99%

Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide

1985

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“…Quantitative starch assays were employed as described by Ebell (1969) with the following modifications. An amount of 0.15 g of fresh leaves was ground in 1 ml 80% ETOH and extracted three times at 80°C.…”

Section: Starch Measurementmentioning

confidence: 99%

Overexpression of TMAC2, a novel negative regulator of abscisic acid and salinity responses, has pleiotropic effects in Arabidopsis thaliana

Huang

2006

Plant Mol Biol

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Phytohormone abscisic acid (ABA) regulates many aspects of plant development and growth. To explore the molecular mechanism of ABA, we identified the novel ABA-regulated genes in Arabidopsis thaliana by searching for genes possessing two or more ABREs (ABA-responsive elements). One of these genes, two or more ABREs-containing gene 2 (TMAC2) is highly induced by ABA and NaC1. Database searches revealed that TMAC2 encodes a protein with no domains of known function. Expression of TMAC2-GFP fusion protein in Arabidopsis mesophyll protoplasts indicated that TMAC2 is targeted to the nucleus. Although the gene has a basal level of expression in various Arabidopsis organs/tissues except for adult leaves, a high expression level was detected in roots. Constitutive overexpression of TMAC2 in plants resulted in the insensitivity to ABA and NaCl, suggesting that TMAC2 plays a negative role in ABA and salt stress responses. Furthermore, TMAC2-overexpressing plants exhibited the short roots, late flowering and starch-excess phenotypes. RT-PCR analysis showed that decreased expression of two floral- and one starch degradation-related genes, SOC1/AGL20 and SEP3/AGL9, and SEX1, respectively, may lead to altered phenotypes of TMAC2-overexpressing plants. Taken together, our data reveal that TMAC2 acts in the nucleus and is an important negative regulator of ABA and salt stress responses, and could play a critical role in controlling root elongation, floral initiation and starch degradation.

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“…Quantitative starch assays were conducted according to Ebell (1969) with the following modifications. Leaf tissue was ground in 80% ethano1 and extracted three times at 8OOC.…”

Section: Starch Measurementmentioning

confidence: 99%

Monogenic Recessive Mutations Causing Both Late Floral Initiation and Excess Starch Accumulation in Arabidopsis.

et al. 1995

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A recessive Arabidopsis mutation, carbohydrate accumulation mutantl (caml), which maps to position 22.8 on chromosome 3, was identified by screening leaves of ethyl methanesulfonate-mutagenized M2 plants stained with iodine for altered starch content. lncreased starch content in leaves of the c a m l mutant was observed at the onset of flowering. This mutant also had a delayed floral initiation phenotype with more rosette leaves than the parenta1 line. In addition, activities of several enzymes associated with starch metabolism were altered in the c a m l mutant. The late-flowering mutant gigantea (gi) also manifested an elevated starch leve1 in leaves. However, not all late-flowering mutants had increased leaf starch content. Double mutants c a m l a d g l (for AJP-glucose pyrophosphorylase), c a m l pgm (for -phosphoglucomutase), and g i p g m had no observable starch in leaves but showed the late-flowering phenotype, demonstrating tThat the elevated starch content is not the cause of late floral initiation. The pleiotropic effects of c a m l and gi suggest that they may play regulatory roles in starch metabolism and floral initiation. These data suggest that starch accumulation and floral initiation may share a common regulatory pathway.

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Specific total starch determinations in conifer tissues with glucose oxidase

Cited by 43 publications

References 26 publications

Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide

Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide

Overexpression of TMAC2, a novel negative regulator of abscisic acid and salinity responses, has pleiotropic effects in Arabidopsis thaliana

Monogenic Recessive Mutations Causing Both Late Floral Initiation and Excess Starch Accumulation in Arabidopsis.

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