Specific spin-labeling of transfer ribonucleic acid molecules

Caron, Marcel; Dugas, Hermann

doi:10.1093/nar/3.1.19

Cited by 45 publications

(35 citation statements)

References 22 publications

(28 reference statements)

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“…4). Motion of mRNA induces T^C G to move to the back of the slot and to become exposed to bind ing with 50S RNA, while pulling the aa end of tRNA forward, see also results obtained by spin label studies by Caron and Dugas [11]. Support 6 .…”

Section: Experimental Findings Supporting the Dynamic Model Predictionsmentioning

confidence: 78%

On the Dynamic Model of tR N A : Recent Experimental Findings

Gürel

1979

Zeitschrift Für Naturforschung C

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Recent experimental findings are shown to support various aspects of the dynamic model of tRNA proposed earlier and the interaction between tRNA, mRNA and the ribosomal RNA’s. Extra loop of tRNA molecule is suggested to play a role in recognizing the corresponding amino acids and a correlation is presented between the tRNA molecules and the corresponding amino acids as tabulated by the genetic tableau.

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Section: Experimental Findings Supporting the Dynamic Model Predictionsmentioning

confidence: 78%

On the Dynamic Model of tR N A : Recent Experimental Findings

Gürel

1979

Zeitschrift Für Naturforschung C

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“…For these reasons, the 5ps labeling method is the only available means to attach a nitroxide at the 5′ terminus of these naturally occurring nucleic acid molecules. Compared to a previously reported method that utilizes periodate oxidation to attach nitroxides to the 3′-terminus of tRNA (22), the 5ps method is application to both RNA and DNA, and thus is applicable to a wide array of important systems, such as methylated DNA (27), ribosomal RNA (28), spliceosomal RNA (29) and non-coding RNA (30). …”

Section: Discussionmentioning

confidence: 99%

“…Methods have also been reported for attaching nitroxides to RNA terminus (11,22). Shin and co-workers have reported a method for attaching nitroxides to the 5′ terminus of a RNA synthesized by the T7 RNA polymerase (11).…”

Section: Introductionmentioning

confidence: 99%

“…The labeling yield depends on the fraction of RNA transcripts containing the 5′-phosphorothioate, which cannot be 100% due to the limit imposed by GMPS to GTP ratio. For labeling at 3′ terminus, there was a report on tRNA (22), which utilizes periodate to oxidize the 3′-ribose, followed by subsequent nitroxide attachment. However, this method cannot be applied directly to DNA.…”

Section: Introductionmentioning

confidence: 99%

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A facile method for attaching nitroxide spin labels at the 5′ terminus of nucleic acids†

Grant

Qin

2007

Nucleic Acids Research

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In site-directed spin labeling (SDSL), a nitroxide moiety containing a stable, unpaired electron is covalently attached to a specific site within a macromolecule, and structural and dynamic information at the labeling site is obtained via electron paramagnetic resonance (EPR) spectroscopy. Successful SDSL requires efficient site-specific incorporation of nitroxides. Work reported here presents a new method for facile nitroxide labeling at the 5′ terminus of nucleic acids of arbitrary sizes. T4-polynucleotide kinase was used to enzymatically substitute a phosphorothioate group at the 5′ terminus of a nucleic acid, and the resulting phosphorothioate was then reacted with an iodomethyl derivative of a nitroxide. The method was successfully demonstrated on both chemically synthesized and naturally occurring nucleic acids. The attached nitroxides reported duplex formation as well as tertiary folding of nucleic acids, indicating that they serve as a valid probe in nucleic acid studies.

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“…First, we have used the intact 70s ribosomes and labeled them under conditions similar to those used to label the 3'-end of tRNA molecules (26). The labelling was directed towards the terminal ribose residue by reductive acylation in the presence of amino-SL (AMSL) and NaBH4, using material pretreated with NaI04.…”

Section: Selective Spin Labelling Of Ribosomesmentioning

confidence: 99%

Spin labelling ribosomes: an overview

Dugas¹,

Rodríguez²

1982

Can. J. Chem.

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This paper reviews the spin label approach to the problem of structure and function of the bacterial ribosomes from Escherichiacoli. The work of different research groups towards selective spin labelling of 70S ribosomes is presented and results are compared. Information obtained by binding these derivatized ribosomes with magnesium ions, tRNA, and antibiotics are analyzed. The interaction of selectively spin-labeled tRNAphe and spin-labeled kanamycin and puromycin with the ribosomes is also discussed.

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Specific spin-labeling of transfer ribonucleic acid molecules

Abstract: The spin labels anhydride (ASL), bromoacetamide $BSL) and carbodiimide (CSLhwere used to label selectively tRNAGu u, tRNAfMet and tRNA ke from E.coli. The preparation and characte-

Cited by 45 publications

References 22 publications

On the Dynamic Model of tR N A : Recent Experimental Findings

On the Dynamic Model of tR N A : Recent Experimental Findings

A facile method for attaching nitroxide spin labels at the 5′ terminus of nucleic acids†

Spin labelling ribosomes: an overview

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