Specific solubilization of impurities in culture media: Arg solution improves purification of pH-responsive tag CspB50 with Teriparatide

Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials

“…Proteins fused with the CspB50 tag were shown to reversibly precipitate at acidic pH, thus being purified using a column-free method with yields and purity comparable to those obtained with conventional affinity purification systems. According to the authors, advantages of the CspB50 tag over ELP tags are: (i) the aggregating-state is fully reversible, (ii) it has a sharp pH responsivity, and (iii) it can have multipurpose applications [105] , [106] .…”

The pH-responsive precipitation–redissolution of the CspB fusion protein, CspB50TEV-Teriparatide, triggered by changes in secondary structure

“…The pH-responsive precipitation–redissolution of CspB fusion proteins is also affected by the solution conditions. In the presence of arginine, the solubility of the impurities increases, which can improve the purification yield of CspB fusion proteins [ 9 ]. Furthermore, kosmotropic ions can shift the pH value at which the CspB fusion protein is precipitated to neutral or basic conditions [ 10 ].…”