Specific serum IgG at diagnosis of Staphylococcus aureus bloodstream invasion is correlated with disease progression

Abstract

S. aureus is a dangerous pathogen of ever increasing importance both in hospitals and in the community. Due to the crisis of antibiotic resistance, an urgent need exists for new strategies to combat S. aureus infections, such as vaccination. To date, however, all vaccine trials have failed in clinical studies. It is therefore unclear whether the adaptive immune system is at all able to control S. aureus in humans. The paper demonstrates the use of proteomics for providing an answer to this crucial question. It describes novel results of a prospective study in patients with S. aureus infection complicated by bloodstream invasion. Immune proteomic analysis shows that robust immune memory of S. aureus - reflected by strong serum IgG antibody binding to S. aureus antigens - is associated with clinical protection from sepsis. This lends support to the notion of a vaccine to protect against the most serious complications of S. aureus infection. Hence, the data encourage further efforts in vaccine development.

Innate Immune Memory Contributes to Host Defense against Recurrent Skin and Skin Structure Infections Caused by Methicillin-Resistant Staphylococcus aureus

“…In humans, antibodies generated against many S. aureus antigens are prevalent and long-lasting (39). However, considerable discordance exists between the humoral response and protective immunity to S. aureus.…”

Розвиток Імунної Відповіді При Стафілококовій Пневмонії (Частина 8)

“…Sebastian Stentzel и со-авт. [59] продемонстрировали, что у 95-100 % па-циентов с бактериемией IgG-антительный ответ индуцируется в отношении нескольких стафило-кокковых антигенов, включая иммунные модуля-торы: стафилококкового суперантигенподобного протеина 3 (staphylococcal superantigen-like protein 3 -SSL3), SSL10, протеина уклонения от иммунно-го ответа SCIN (staphylococcal complement inhibitor), γ-гемолизина (Hlγ), лейкоцидина F (LukF), АВС транспортера SA0688, мембранно-ассоциирован-ной фолдазы PrsA и связанного с поверхностью мембраны и внеклеточного энзима IsaA [18].…”

“…Пациенты с благо-приятным исходом заболевания характеризуются более высокими уровнями антител, направленных против внеклеточных ферментов IsaA, Plc и GlpQ, а также гомолога Eap EapH2 (SACOL0985) бактерий Staphylococcus aureus [59].…”

Omics Approaches for the Study of Adaptive Immunity to Staphylococcus aureus and the Selection of Vaccine Candidates

“…They are enriched for virulence factors, immune evasins and adhesins [123], which are predominantly targeted by the humoral immune response and thus represent candidates for antibody-based vaccines [133]. By dissecting the exoproteomes of 25 clinical S. aureus isolates, Ziebandt et al discovered that their composition was extremely variable; only eight proteins were shared by all isolates [134].…”

“…Building on prior knowledge about the antigenic composition of S. aureus , immunogenic S. aureus proteins can be recombinantly expressed for (1) validation of their role as prominent antibody targets and (2) integration into multiplex assays permitting high throughput quantification of specific antibodies (Table 1) [41,133,161,162]. Most multiplex assays used in S. aureus research are based on suspension array technology (e.g., Luminex ® ) that allows simultaneous quantification of antibody binding to up to 500 bacterial proteins over a large dynamic range (10^5).…”