Specific screening methods for glucosinolates in sprout extracts using on-line thermospray liquid chromatography-tandem mass spectrometry

Heeremans, C. E. M.; Hoeven, R. A. M. van der; Niessen, W.M.A.; Vuik, Johan; Vos, Rudi H. De; Greef, J. van der

doi:10.1016/s0021-9673(00)94108-6

Cited by 16 publications

(3 citation statements)

References 12 publications

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“…In contrast to the techniques above, TSP is a quite old interface/ionization system and therefore much more sensitive to the influence of experimental conditions such as vaporizer temperature, block temperature, composition of the mobile phase, flow rate, and performance of the spray, so that it is in some cases impossible to reproduce results from day to day (Heeremans et al, 1989).…”

Section: Identification Of Desulfoglucosinolates By Esi-ms-msmentioning

confidence: 99%

Glucosinolates in Members of the Family Brassicaceae: Separation and Identification by LC/ESI-MS-MS

Matthäus

Luftmann

2000

J. Agric. Food Chem.

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Seeds of 14 different members of the family Brassicaceae were investigated with regard to their content and composition of glucosinolates by HPLC-UV/ESI-MS-MS coupling. The seeds were extracted with hot methanol/water (70:30 v/v) and the desulfoglucosinolates isolated by anion-exchange chromatography with solid-phase extraction columns. The desulfoglucosinolates were detected by UV and identified by ESI-MS/MS with the neutral loss method. Nineteen different glucosinolates were detected in the seeds with a wide range of contents (10-200 micromol/g) and a great variation in the composition.

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Section: Identification Of Desulfoglucosinolates By Esi-ms-msmentioning

confidence: 99%

Glucosinolates in Members of the Family Brassicaceae: Separation and Identification by LC/ESI-MS-MS

Matthäus

Luftmann

2000

J. Agric. Food Chem.

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“…In the earliest report of LC/MS analysis of desulfoglucosinolates (15), thermospray ionization was used to confirm the structures of molecules isolated from seeds of Brassica campestris. Thermospray LC/MS has subsequently been used to identify desulfoglucosinolates isolated from Calabrese leaves (16), Arabidopsis thaliana leaves (17,18), sprout extracts (19), rape (20,21), and broccoli (22). Additional structural information was obtained by MS/MS in one of the above examples (19).…”

mentioning

confidence: 99%

“…Thermospray LC/MS has subsequently been used to identify desulfoglucosinolates isolated from Calabrese leaves (16), Arabidopsis thaliana leaves (17,18), sprout extracts (19), rape (20,21), and broccoli (22). Additional structural information was obtained by MS/MS in one of the above examples (19). GC/MS and thermospray LC/MS methods for analyzing glucosinolates have also been described and compared (23).…”

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confidence: 99%

Intact Glucosinolate Analysis in Plant Extracts by Programmed Cone Voltage Electrospray LC/MS: Performance and Comparison with LC/MS/MS Methods

Mellon

Bennett

Holst

et al. 2002

Analytical Biochemistry

115

104

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Glucosinolates in Crop Plants

Rosa

Heaney

Fenwick

et al. 1996

Horticultural Reviews

172

170

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Glucosinolates with Brassica genus as secondary metabolites have a lot of functions and effects. Glucosinolates form less than 2% of the overall sulphur content at the beginning of vegetation in different parts of the plants and during growth their content is decreasing and forms less than 0.1%. This low representation doubts their storage function. With its chemical composition, they are ranked among natural pesticides with active and passive resistance against diseases and pests. They show repellent effects and properties of natural biofumigators in soil after ploughing in their biomass as green fertilizing, or after ploughing in after harvest the leftovers of rape. The principle of these effects is decomposition products of glucosinolates-bioactive isothiocyanates. Very important from this point of view are turnip rape Rex and Brassica juncea, whose content of these compounds is the highest one and they are resistant against the attack of Ceutor-rhynchus pleurostigma. The same effect showed also when attacked by Phoma lingam. With other winter Brassicas either hybrid or linea and summer rape is this defensive system suppressed because of their lowered content due to breeding interferences, leading to limitation of their anti-nutritional negative effects. It is possible to state the final result after finding out the production of the above matter, roots, and after evaluation of the sorbal characteristics of the soil and evaluation of the state of health of the following crop or vegetable. After this overall analysis, it will be possible to evaluate the biofumigation properties of accessible varieties of the Brassica genus.

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Specific screening methods for glucosinolates in sprout extracts using on-line thermospray liquid chromatography-tandem mass spectrometry

Cited by 16 publications

References 12 publications

Glucosinolates in Members of the Family Brassicaceae: Separation and Identification by LC/ESI-MS-MS

Glucosinolates in Members of the Family Brassicaceae: Separation and Identification by LC/ESI-MS-MS

Intact Glucosinolate Analysis in Plant Extracts by Programmed Cone Voltage Electrospray LC/MS: Performance and Comparison with LC/MS/MS Methods

Glucosinolates in Crop Plants

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