Specific response of human lymphocytes to pollen antigen in tissue culture

Zeitz, Stanley J.; VanArsdel, Paul P.; McClure, Donald K.

doi:10.1016/0021-8707(66)90030-x

Cited by 28 publications

(11 citation statements)

References 27 publications

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“…show that elevated PBL proliferation in response to the causative allergens has a biological role(s) in developing clinical symptoms (9)(10)(11)(12)(13)(14). In this study, we observed stronger PBL proliferation in response to CYA in adult asthmatic patients than normal controls.…”

Section: Discussionsupporting

confidence: 60%

Analysis of Lymphocyte Response to Chironomid Midge Antigens in Asthmatic and Non-Asthmatic Individuals

Edahiro

Ohta

Matsuoka

et al. 1989

JJMSB

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Section: Discussionsupporting

confidence: 60%

Analysis of Lymphocyte Response to Chironomid Midge Antigens in Asthmatic and Non-Asthmatic Individuals

Edahiro

Ohta

Matsuoka

et al. 1989

JJMSB

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“…These include antigen-induced lymphocyte transformation and measuring the elaboration of lymphocyte mediators (MIF and mitogenic factor). Other investigators have previously shown that lymphocytes from atopic patients with summer or-fall hayfever transformed and/ or incorporated increased amounts of [3H]thymidine in vitro (1)(2)(3)(4)(5) and produced mediators (4)(5)(6) in response to grass or ragweed pollen antigens. We found that lymphocytes from 22/28 patients with ragweed hayfever incorporated increased amounts of [3H]thymidine in response to RAgE.…”

Section: Discussionmentioning

confidence: 99%

“…Received for publication 1 Auguist 1973 and in revised form 22 October 1973. tivity in man. Clinical responses to ragweed antigen have been shown to primarily involve reaginic (IgE) antibody.…”

Section: Introductionmentioning

confidence: 99%

“…To what extent components of cellular immunity (delayed hypersensitivity) are involved in this response, however, is not altogether clear. Since it is often difficult to elicit positive delayed hypersensitivity skin tests after the intradermal injection of pollen antigens, some investigators have elected to study in vitro lymphocyte responses to certain pollens as a correlate of in vivo hypersensitivity (1)(2)(3)(4)(5)(6). Some of the techniques which measure in vitro lymphocyte activation include the incorporation of ['H]thymidine into cellular DNA (7) and the detection of soluble mediators such as migration inhibitory factor (MIF)' and mitogenic (lymphocyte transforming) factor (8).…”

Section: Introductionmentioning

confidence: 99%

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Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Rocklin¹,

Pence²,

Kaplan³

et al. 1974

J. Clin. Invest.

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A B S T R A C T The in vivo and in vitro responses to ragweed antigen E were evaluated in 28 untreated atopic patients with ragweed hayfever. The methods employed included direct skin testing, measurement of total serum IgE, measurement of specific IgE anti-ragweed antibodies, leukocyte histamine release, lymphocyte transformation, and release of lymphocyte mediators (migration inhibitory factor and mitogenic factor). The patients could be divided into sensitive and insensitive groups on the basis of their in vitro reactivity to antigen E. 20 patients in the sensitive group had statistically higher levels of total serum IgE, higher levels of specific IgE anti-ragweed antibodies, and greater leukocyte sensitivity as measured by antigen-induced histamine release than did eight patients in the insensitive group. Lymphocytes from sensitive patients produced greater amounts of migration inhibitory factor and mitogenic factor when challenged by antigen E than did lymphocytes from insensitive patients. A possible role for the lymphocyte in this allergic disease is discussed. serum IgE levels of paIgEAR levels but normal total serum IgE levels. Of interest was the finding that a small number of patients had diminished lymphocyte mediator production, histamine release, IgE, and IgEAR levels. One could not distinguish clinically between these two groups. METHODSPatients. 28 atopic patients with symptoms compatible with seasonal allergic rhinitis and no previous history of receiving ragweed immunotherapy were included in this study. The ages ranged from 15 to 64 yr (mean 28.9 yr) and included 23 males and 5 females. 18 nonatopic subjects served as controls.Skin tests. At one forearm site 0.05 ml of aqueous ragweed extract (100 protein nitrogen units/ml) alone was injected intradermally. In the opposite forearm 0.05 ml chlorpheniramine maleate (10 mg/ml) was injected before the subsequent intradermal injection of 0.05 ml ragweed extract at the same site. At a third site chlorpheniramine alone was injected (0.05 ml). The amount of erythema and induration were measured at each site at 15 min and 48 h. In one subject a punch biopsy specimen was obtained at 48 h from sites injected with ragweed extract plus chlorpheniramine and chlorpheniramine alone.Serum IgE levels. Serum samples were obtained from patients at the time of initial evaluation and IgE levels determined by use of a Phadebas kit purchased from Pharmacia (Uppsala, Sweden). Serum samples for IgE levels were also obtained from 50 subjects in the same age range as the patient population.Specific IgEAR levels. The radioallergosorbent test (RAST) was used to measure specific IgEAR levels (9). 10 ml of a 20% whole ragweed extract was conjugated to 500 mg of cyanogen bromide-activated cellulose particles (10). 25-, 50-, and 75-il samples of sera were added to 0.5-mg aliquots of the antigen-cellulose conjugate and incubated overnight in a horizontal rotator. After washing, 0.05 ml of "I-labeled immunosorbent purified anti-IgE (Fc) was added to each tube. The anti-IgE (...

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“…Early experiments indicated that the phe nomenon depended on the presence of active delayed hypersensitivity [3,4]. It was subsequently found that lymphocytes from patients showing only immediate hypersensitivity also underwent antigen induced transfor mation, and that the presence or absence of either 7S or 19S antibody was of no consequence [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Antigen Mediated Transformation of Rhesus Lymphocytes in Immediate and Delayed Hypersensitivity

Mackler¹,

Malley²,

Amkraut³

1971

Int Arch Allergy Immunol

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In vitro lymphocyte transformation was demonstrated using peripheral lymphocytes from rhesus monkeys showing either ‘pure’ atopic (Ascaris induced) or ‘pure’ delayed (p-azobenzenearsono-N-acetyl tyrosine induced) hypersensitivity. Lymphocytes from animals immunized by intravenous injection of 2, 4, 6-trinitrophenyl-Keyhole Limpet Hemocyanin (TNP-KLH) did not show transformation either during the active phase of antibody production or at any time thereafter. However, lymphocytes obtained from animals immunized with TNP-KLH in complete Freund’s adjuvant showed transformation not only with TNP-KLH but also with the hapten (TNP) on a heterologous carrier. The relationship of these observations to cell proliferation without MIF production and to the avidity of antigen-cell interaction needed for in vitro transformation is discussed.

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Specific response of human lymphocytes to pollen antigen in tissue culture

Cited by 28 publications

References 27 publications

Analysis of Lymphocyte Response to Chironomid Midge Antigens in Asthmatic and Non-Asthmatic Individuals

Analysis of Lymphocyte Response to Chironomid Midge Antigens in Asthmatic and Non-Asthmatic Individuals

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Antigen Mediated Transformation of Rhesus Lymphocytes in Immediate and Delayed Hypersensitivity

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