Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta

Hirata, Yukio; Tomita, Masahiro; Yoshimi, Hiroki; Ikeda, Masao

doi:10.1016/0006-291x(84)90576-x

Cited by 208 publications

(69 citation statements)

References 11 publications

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“…The Scatchard analysis was applied to the saturable binding of 1251-~-ANP to VSMC and gave an apparent dissociation constant (Kd) of 0.87 x 10e9 M and the number of maximum binding sites 30 (Bmax) of 230000 sites/cell. These values are well comparable to those reported in the literature (Kd = 1.36 x 10m9 M, B,, = 220000 sites/cell) [9]. The competition of '251-a-ANP with unlabeled (Y-ANP for binding to VSMC was also analyzed by the Scatchard plots to give Kd = 1.2 x 10m9 M and B,,, = 289 000 sites/cell (literature: Kd = 2.4 x 10m9 M, Bmax = 278000 sites/cell 191).…”

Section: Characterization Of Vsmcsupporting

confidence: 91%

“…In this respect we have been trying to measure cGMP production in VSMC for characterization of synthetic ANP analogs. The cultured cells used were found to be identical with those established by Hirata et al [9] with respect to the dissociation constant and the maximum binding sites for a-ANP.…”

Section: Discussionsupporting

confidence: 58%

“…cu-ANP has specific receptors on cultured rat VSMC and is known to stimulate intracellular guanylate cyclase [9]. In this respect we have been trying to measure cGMP production in VSMC for characterization of synthetic ANP analogs.…”

Section: Discussionmentioning

confidence: 99%

“…Vascular smooth muscle cells (VSMC) obtained from the thoracic aorta of 20-week-old male Wistar rats were cultured in virtually the same manner as described by Hirata et al [9]. Subcultured VSMC between 6th and 10th passages were used in the present experiments.…”

Section: Culture Of Rat Vsmcmentioning

confidence: 99%

“…The rat VSMC culture prepared according to Hirata et al [9] showed a so-called hill-and-valley structure characteristic of smooth muscle cells. The Scatchard analysis was applied to the saturable binding of 1251-~-ANP to VSMC and gave an apparent dissociation constant (Kd) of 0.87 x 10e9 M and the number of maximum binding sites 30 (Bmax) of 230000 sites/cell.…”

Section: Characterization Of Vsmcmentioning

confidence: 99%

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A dicarba analog of β‐atrial natriuretic peptide (β‐ANP) inhibits guanosine 3′,5′‐cyclic monophosphate production induced by α‐ANP in cultured rat vascular smooth muscle cells

et al. 1989

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The synthesis and biological properties are described of [As~'~~']-/l-ANP-(7-28) (Asu, L-a-aminosuberic acid), a dicarba analog of p-atria1 natriuretic peptide (8-ANP, an antiparallel dimer of human a-ANP with the chains linked by 7-23' and 7-23 disulfide bonds). This Asu-analog (referred to as analog III) displaced 12SI-a-ANP specifically bound to cultured rat vascular smooth muscle cells (VSMC) with an apparent Ki of 2.1 x 10-s M, but did not stimulate formation of intracellular cGMP at 10-8-10-5 M. Analog III inhibited the a-ANP-stimulated cGMP production in VSMC competitively with a pA, value of 7.45 and behaved as an antagonist of a-ANP in rat aorta smooth muscle relaxation. In addition, /I-ANP was also shown to inhibit the a-ANP-induced cGMP production in a dose-dependent manner. The mechanism of action of /I-ANP is also discussed.Atria1 natriuretic peptide analog; Atria1 natriuretic peptide receptor antagonist; Receptor binding; cyclic GMP production; Vasorelaxation; (Cultured vascular smoothmuscle cell)

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Section: Characterization Of Vsmcsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Culture Of Rat Vsmcmentioning

confidence: 99%

Section: Characterization Of Vsmcmentioning

confidence: 99%

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A dicarba analog of β‐atrial natriuretic peptide (β‐ANP) inhibits guanosine 3′,5′‐cyclic monophosphate production induced by α‐ANP in cultured rat vascular smooth muscle cells

et al. 1989

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Nitric oxide releasing reagent, S-nitroso-N-acetylpenicillamine, enhances the expression of manganese superoxide dismutase mRNA in rat vascular smooth muscle cells

et al. 1996

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Cultured rat vascular smooth muscle cells (VSMCs) produce nitric oxide (NO) under stimulation by lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma). NO synthase (NOS) and manganese superoxide dismutase (Mn-SOD) mRNA expressions are simultaneously induced by these stimulants in rat VSMCs. In VSMCs, S-nitroso-N-acetyl penicillamine (SNAP), one of the NO releasing reagents, induces Mn-SOD mRNA which may protect the VSMCs themselves. This suggests that NO itself may enhance the expression of Mn-SOD to protect the VSMC themselves against NO radicals in cultured rat VSMCs.

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Localization of atrial natriuretic factor (ANF) binding sites in the central nervous system of the frog

Tong

Netchitaïlo

Leboulenger

et al. 1989

J of Comparative Neurology

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The distribution of atrial natriuretic factor (ANF) binding sites was investigated in the central nervous system of the frog Rana ridibunda using the technique of in vitro receptor autoradiography by means of [125I]-labeled ANF-28. The anatomic distribution of ANF recognition sites was determined on Kodak ARX films apposed onto tissue sections, and their distribution was examined in greater detail by analysis of autoradiograms generated by using emulsion-coated sections. The highest levels of ANF binding sites were found in the olfactory bulb, the dorsal pallium, the septum, the habenular nucleus, the dorsal infundibular nucleus, the interpeduncular nucleus, and in the tectum. Moderate levels of ANF binding sites were observed in the thalamus and throughout the mesencephalon, whereas low levels were detected in the lateral and medial pallium, the medial forebrain bundle, and the nucleus rotondus. In the pituitary gland, the neural and distal lobes were densely loaded with ANF binding sites, whereas no autoradiographic labeling was observed in the pars intermedia. In general, there was a good correlation between the location of ANF receptors and the distribution of ANF-containing neurons, as previously determined by immunocytochemistry. Together these results support the view that ANF may act as a neurotransmitter or a neuromodulator in various regions of the frog brain.

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Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta

Cited by 208 publications

References 11 publications

A dicarba analog of β‐atrial natriuretic peptide (β‐ANP) inhibits guanosine 3′,5′‐cyclic monophosphate production induced by α‐ANP in cultured rat vascular smooth muscle cells

A dicarba analog of β‐atrial natriuretic peptide (β‐ANP) inhibits guanosine 3′,5′‐cyclic monophosphate production induced by α‐ANP in cultured rat vascular smooth muscle cells

Nitric oxide releasing reagent, S-nitroso-N-acetylpenicillamine, enhances the expression of manganese superoxide dismutase mRNA in rat vascular smooth muscle cells

Localization of atrial natriuretic factor (ANF) binding sites in the central nervous system of the frog

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