Specific quorum sensing molecules are possibly associated with responses to herbicide toxicity in a Pseudomonas strain

Freitas, Paloma Nathane Nunes de; Rovida, Amanda Flávia da Silva; Silva, Caroline Rosa; Pileggi, Sônia Alvim Veiga; Olchanheski, Luiz Ricardo; Pileggi, Marcos

doi:10.1016/j.envpol.2021.117896

Cited by 7 publications

(8 citation statements)

References 67 publications

(65 reference statements)

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“…Although 2,4-D influences the structure of microbiomes in soil, even with a low half-life [ 26 ], there are few studies that describe the effects of 2,4-D on bacteria of the genus Pseudomonas , reporting degradation processes, without describing bacterial systems of tolerance to the herbicide [ 27 ]. One exception is the description of the role of quorum sensing signaling molecules in the 2,4-D response to 2,4-D [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…The enzymatic control of H 2 O 2 is poorly documented for bacteria, and this work is unprecedented for Pseudomonas and 2,4-D, firstly describing the enzymatic role of APX and GPX in assisting CAT in H 2 O 2 control in response to herbicides in bacteria. Since there are reports of coordinated gene regulation between SOD and CAT isoforms in Pseudomonas through quorum sensing signaling molecules [ 44 ], and the types and number of these molecules depend on the bacterial density and the presence of herbicides, including 2,4-D [ 28 ], it is possible that the quorum sensing is also related to the coordinated activities of CAT, APX, and GPX in Pseudomonas sp. CMA-7.3.…”

Section: Resultsmentioning

confidence: 99%

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Tolerance of Pseudomonas strain to the 2,4-D herbicide through a peroxidase system

et al. 2021

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Herbicides are widely used in agricultural practices for preventing the proliferation of weeds. Upon reaching soil and water, herbicides can harm nontarget organisms, such as bacteria, which need an efficient defense mechanism to tolerate stress induced by herbicides. 2,4-Dichlorophenoxyacetic acid (2,4-D) is a herbicide that exerts increased oxidative stress among bacterial communities. Bacterial isolates were obtained from the biofilm of tanks containing washing water from the packaging of different pesticides, including 2,4-D. The Pseudomonas sp. CMA-7.3 was selected because of its tolerance against 2,4-D toxicity, among several sensitive isolates from the biofilm collection. This study aimed to evaluate the antioxidative response system of the selected strain to 2,4-D. It was analyzed the activity of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase GPX enzymes, that are poorly known in the literature for bacterial systems. The Pseudomonas sp. CMA-7.3 presented an efficient response system in balancing the production of hydrogen peroxide, even at 25x the dose of 2,4-D used in agriculture. The antioxidative system was composed of Fe–SOD enzymes, less common than Mn–SOD in bacteria, and through the activities of KatA and KatB isoforms, working together with APX and GPX, having their activities coordinated possibly by quorum sensing molecules. The peroxide control is poorly documented for bacteria, and this work is unprecedented for Pseudomonas and 2,4-D. Not all bacteria harbor efficient response system to herbicides, therefore they could affect the diversity and functionality of microbiome in contaminated soils, thereby impacting agricultural production, environment sustainability and human health.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Tolerance of Pseudomonas strain to the 2,4-D herbicide through a peroxidase system

et al. 2021

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“…A more accurate definition of this issue can improve the understanding of the turnover of glyphosate in environment and xenobiotic bioremediation strategies. The strain P. fluorescens CMA-55 was also studied by Freitas et al. (2021) , noting that it had a profile of quorum sensing signaling molecules involved in controlling the production of reactive oxygen species for saflufenacil and glyphosate herbicides, and involved in different stages of biofilm formation in the presence of sulfentrazone, 2,4-D and dicamba herbicides.…”

Section: Resultsmentioning

confidence: 99%

“…The bacterium used in this study, P. fluorescens CMA-55, was selected for being an isolate to show the best growth rates at a concentration of 50x glyphosate. This strain was previously evaluated for the production of specific quorum sensing signaling molecules in response to herbicides, including glyphosate ( Freitas et al., 2021 ). It was obtained from the Collection of Environmental Microorganisms at the Laboratory of Environmental Microbiology of Ponta Grossa State University, Brazil, which had been isolated from biofilms formed in storage tanks of water used for washing herbicide containers ( Lima et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens

Oliveira

Marchi

Emiliano

et al. 2022

Heliyon

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“…Pseudomonas aeruginosa is an example of an opportunistic human bacterium that causes devastating infections in patients with compromised immune systems, and its ability to form antibiotic-resistant biofilms it is probably the reason for the persistence in clinical settings ( Thi et al, 2020 ). Novel approaches have shown the interference of quorum sensing molecules in other functions, such as coordinate response systems, as antioxidative enzymes production or biofilm formation to tolerate herbicides ( Freitas et al, 2021 ). There is great diversity in communication networks mediated by quorum sensing signals, including in virulence modulation, therefore have become a promising target for mitigating pathogens ( Contreras-Ramos and Mansell, 2021 ).…”

Section: Literature Reviewmentioning

confidence: 99%

Probiotics and live biotherapeutic products aiming at cancer mitigation and patient recover

Schemczssen-Graeff

Pileggi

2022

Front. Genet.

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Molecular biology techniques allowed access to non-culturable microorganisms, while studies using analytical chemistry, as Liquid Chromatography and Tandem Mass Spectrometry, showed the existence of a complex communication system among bacteria, signaled by quorum sensing molecules. These approaches also allowed the understanding of dysbiosis, in which imbalances in the microbiome diversity, caused by antibiotics, environmental toxins and processed foods, lead to the constitution of different diseases, as cancer. Colorectal cancer, for example, can originate by a dysbiosis configuration, which leads to biofilm formation, production of toxic metabolites, DNA damage in intestinal epithelial cells through the secretion of genotoxins, and epigenetic regulation of oncogenes. However, probiotic strains can also act in epigenetic processes, and so be use for recovering important intestinal functions and controlling dysbiosis and cancer mitigation through the metabolism of drugs used in chemotherapy, controlling the proliferation of cancer cells, improving the immune response of the host, regulation of cell differentiation and apoptosis, among others. There are still gaps in studies on the effectiveness of the use of probiotics, therefore omics and analytical chemistry are important approaches to understand the role of bacterial communication, formation of biofilms, and the effects of probiotics and microbiome on chemotherapy. The use of probiotics, prebiotics, synbiotics, and metabiotics should be considered as a complement to other more invasive and hazard therapies, such chemotherapy, surgery, and radiotherapy. The study of potential bacteria for cancer treatment, as the next-generation probiotics and Live Biotherapeutic Products, can have a controlling action in epigenetic processes, enabling the use of these bacteria for the mitigation of specific diseases through changes in the regulation of genes of microbiome and host. Thus, it is possible that a path of medicine in the times to come will be more patient-specific treatments, depending on the environmental, genetic, epigenetic and microbiome characteristics of the host.

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Specific quorum sensing molecules are possibly associated with responses to herbicide toxicity in a Pseudomonas strain

Cited by 7 publications

References 67 publications

Tolerance of Pseudomonas strain to the 2,4-D herbicide through a peroxidase system

Tolerance of Pseudomonas strain to the 2,4-D herbicide through a peroxidase system

Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens

Probiotics and live biotherapeutic products aiming at cancer mitigation and patient recover

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