Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas

Lurz, Rudolf; Rückert, Beate; Franklin, F. Christopher H.; Bagdasarian, Michael; Frey, Joachim; Timmis, Kenneth N.

doi:10.1016/0378-1119(81)90080-9

Cited by 1,068 publications

(396 citation statements)

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“…P. putida strains KT2440, 16 and KT2440.2431, 17 were used as host bacteria. P. putida KT2440 is a ubiquitous saprophytic bacterium endowed with a remarkable adaptability to diverse environments and is thus suitable for the (4) The bacterial lux operon encodes for luciferase and the proteins necessary for substrate synthesis; therefore, luminescence can be measured directly as no additional substrate is required.…”

Section: Resultsmentioning

confidence: 99%

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Improving the sensitivity of bacterial bioreporters for heavy metals

2010

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Section: Resultsmentioning

confidence: 99%

“…As bioreporters, P. putida strains KT2440, 16 and KT2440.2431, 17 were used. Both strains were transformed with the sensor plasmids pDNPcadA1lux and pDNPczc1lux.…”

Section: Methodsmentioning

confidence: 99%

Improving the sensitivity of bacterial bioreporters for heavy metals

2010

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“…Polymerase chain reaction (PCR) was carried out on a PerkinElmer-Cetus DNA thermal cycler using the Taq DNA polymerase from Cetus. On each cycle of amplification, the denaturation, annealing, and polymerization steps were, respectively, carried out for 1 SUMT activity was assayed in cell extracts as previously described (6), except that incubations were carried out at 100…”

Section: Methodsmentioning

confidence: 99%

“…Values ranging from 0.1 to 1.4 ,uLmol g (dry weight)- 1 have been reported (25,43). The latter value corresponds to a high level of corrinoid compared with that in eubacteria such as Streptomyces, Bacillus, Rhizobium, Agrobacterium, or Pseudomonas spp., in which levels significantly lower (the higher values are 0.1 ,umol g [dry weight]-') have been observed (31).…”

mentioning

confidence: 99%

Purification, characterization, and molecular cloning of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Methanobacterium ivanovii

Blanche¹,

Robin²,

Couder³

et al. 1991

J Bacteriol

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An S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) activity has been identified in Methanobacterium ivanovii and was purified 4,500-fold to homogeneity with a 38% yield. The enzyme had an apparent molecular weight of 58,200 by gel filtration and consisted of two identical subunits of Mr 29,000, as estimated by gel electrophoresis under denaturing conditions. The Km value for uroporphyrinogen III was 52 nM. The enzyme catalyzed the two C-2 and C-7 methylation reactions converting uroporphyrinogen III into precorrin-2. Unlike Pseudomonas denitrificans SUMT, the only SUMT characterized to date (F. Blanche, L. Debussche, D. Thibaut, J. Crouzet and B. Cameron, J. Bacteriol. 171:4222-4231, 1989), M. ivanovii SUMT did not show substrate inhibition at uroporphyrinogen Ill concentrations of up to 20 ,uM. Oligonucleotide probes from limited peptide sequence information were used to clone the corresponding gene. The encoded polypeptide showed more than 40% strict homology with P. denitrificans SUMT. The M. ivanovii SUMT structural gene is likely to be, as is P. denitrificans cobA, involved in corrinoid synthesis.Methanogenic bacteria synthesize particular types of corrinoids, mainly 5-hydroxybenzimidazolylcobamide (vitamin B12 factor III) (24,33,34,42,46) and Coa-t[-(7-adenyl)]cobamide (pseudovitamin B12) (24,43). Occurrence of cobina-

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“…The plasmids used were pMC1403 (Alae Ap': lacks a promoter region and the first eight codons of the N-terminal end of the p-galactosidase gene), pKT230 (Sm'Km': a hybrid plasmid between RSF1010 and pACYCI77 constructed by Bagdasarian et al, 7) and RSF1010 (Sm'Su': a broad-host-range plasmid in Gram-negative bacteria). TY medium (tryptone 109, yeast extract 3g, NaCI 5g per liter, pH 7.6) was used for cultivation of the bacteria.…”

Section: Received May 15 1984mentioning

confidence: 99%